S5 lipase

An organic solvent tolerant enzyme

Raja Noor Zaliha Raja Abdul Rahman, Syarul Nataqain Baharum, Abu Bakar Salleh, Mahiran Basri

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

In this study, an organic solvent tolerant bacterial strain was isolated. This strain was identified as Pseudomonas sp. strain S5, and was shown to degrade BTEX (Benzene, Toluene, Ethyl-Benzene, and Xylene). Strain S5 generates an organic solvent-tolerant lipase in the late logarithmic phase of growth. Maximum lipase production was exhibited when peptone was utilized as the sole nitrogen source. Addition of any of the selected carbon sources to the medium resulted in a significant reduction of enzyme production. Lower lipase generation was noted when an inorganic nitrogen source was used as the sole nitrogen source. This bacterium hydrolyzed all tested triglycerides and the highest levels of production were observed when olive oil was used as a natural triglyceride. Basal medium containing Tween 60 enhanced lipase production to the most significant degree. The absence of magnesium ions (Mg2+) in the basal medium was also shown to stimulate lipase production. Meanwhile, an alkaline earth metal ion, Na+, was found to stimulate the production of S5 lipase.

Original languageEnglish
Pages (from-to)583-590
Number of pages8
JournalJournal of Microbiology
Volume44
Issue number6
Publication statusPublished - Dec 2006
Externally publishedYes

Fingerprint

Lipase
Enzymes
Nitrogen
Benzene
Triglycerides
Alkaline Earth Metals
Ions
Xylenes
Peptones
Polysorbates
Toluene
Pseudomonas
Magnesium
Carbon
Bacteria
Growth

Keywords

  • Nutritional factors
  • Pseudomonas sp. organic solvent lipase

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology

Cite this

Rahman, R. N. Z. R. A., Baharum, S. N., Salleh, A. B., & Basri, M. (2006). S5 lipase: An organic solvent tolerant enzyme. Journal of Microbiology, 44(6), 583-590.

S5 lipase : An organic solvent tolerant enzyme. / Rahman, Raja Noor Zaliha Raja Abdul; Baharum, Syarul Nataqain; Salleh, Abu Bakar; Basri, Mahiran.

In: Journal of Microbiology, Vol. 44, No. 6, 12.2006, p. 583-590.

Research output: Contribution to journalArticle

Rahman, RNZRA, Baharum, SN, Salleh, AB & Basri, M 2006, 'S5 lipase: An organic solvent tolerant enzyme', Journal of Microbiology, vol. 44, no. 6, pp. 583-590.
Rahman RNZRA, Baharum SN, Salleh AB, Basri M. S5 lipase: An organic solvent tolerant enzyme. Journal of Microbiology. 2006 Dec;44(6):583-590.
Rahman, Raja Noor Zaliha Raja Abdul ; Baharum, Syarul Nataqain ; Salleh, Abu Bakar ; Basri, Mahiran. / S5 lipase : An organic solvent tolerant enzyme. In: Journal of Microbiology. 2006 ; Vol. 44, No. 6. pp. 583-590.
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