Rubber (Hevea brasiliensis) seed oil toxicity effect and Linamarin compound analysis

Jumat Salimon, Bashar Mudhaffar Abdullah, Nadia Salih

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Background. The lipid fraction of rubber (Hevea brasiliensis (kunth. Muell)) seed was extracted and analyzed for toxicological effect. The toxicological compound such as linamarin in rubber seed oil (RSO) extracted using different solvents, such as hexane (RSOh), mixture of chloroform+methanol (RSOchl+mth) and ethanol (RSOeth) were also studied. Various methods analysis such as Fourier transforms infrared spectroscopy (FTIR) and colorimetric methods were carried out to determine the present of such compounds. Results. FTIR spectrum of RSO did not show any presence of cyanide peak. The determination of cyanide by using colorimetric method was demonstrated no response of the cyanide in RSO and didnt show any colored comparing with commercial cyanide which observed blue color. The results showed that no functional groups such as cyanide (CN) associated with linamarin were observed. Toxicological test using rats was also conducted to further confirm the absence of such compounds. RSO did not show any toxic potential to the rats. Bioassay experiments using shrimps had been used as test organisms to evaluate the toxicity of linamarin extract from RSOh, RSO chl+mth and RSOeth and LC50 were found to be (211.70%, 139.40%, and 117.41%, respectively). Conclusions. This can be attributed no hazardous linamarin were found in RSO.

Original languageEnglish
Article number74
JournalLipids in Health and Disease
Volume11
DOIs
Publication statusPublished - 2012

Fingerprint

Hevea
Oilseeds
Rubber
Cyanides
Toxicity
Seeds
Oils
Toxicology
Fourier Transform Infrared Spectroscopy
Rats
Ethanol
Bioassay
Poisons
Hexanes
Chloroform
Biological Assay
Functional groups
Methanol
Seed
rubber seed oil

Keywords

  • Colorimetric method
  • Linamarin
  • Rats
  • Rubber seed oil
  • Shrimps
  • Toxicity

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical
  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

Cite this

Rubber (Hevea brasiliensis) seed oil toxicity effect and Linamarin compound analysis. / Salimon, Jumat; Abdullah, Bashar Mudhaffar; Salih, Nadia.

In: Lipids in Health and Disease, Vol. 11, 74, 2012.

Research output: Contribution to journalArticle

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abstract = "Background. The lipid fraction of rubber (Hevea brasiliensis (kunth. Muell)) seed was extracted and analyzed for toxicological effect. The toxicological compound such as linamarin in rubber seed oil (RSO) extracted using different solvents, such as hexane (RSOh), mixture of chloroform+methanol (RSOchl+mth) and ethanol (RSOeth) were also studied. Various methods analysis such as Fourier transforms infrared spectroscopy (FTIR) and colorimetric methods were carried out to determine the present of such compounds. Results. FTIR spectrum of RSO did not show any presence of cyanide peak. The determination of cyanide by using colorimetric method was demonstrated no response of the cyanide in RSO and didnt show any colored comparing with commercial cyanide which observed blue color. The results showed that no functional groups such as cyanide (CN) associated with linamarin were observed. Toxicological test using rats was also conducted to further confirm the absence of such compounds. RSO did not show any toxic potential to the rats. Bioassay experiments using shrimps had been used as test organisms to evaluate the toxicity of linamarin extract from RSOh, RSO chl+mth and RSOeth and LC50 were found to be (211.70{\%}, 139.40{\%}, and 117.41{\%}, respectively). Conclusions. This can be attributed no hazardous linamarin were found in RSO.",
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