Roselle supplementation prevents nicotine-induced vascular endothelial dysfunction and remodelling in rats

Lislivia Yiang Nee Si, Kamisah Yusof, Anand Ramalingam, Yi Cheng Lim, Siti Balkis Budin, Satirah Zainalabidin

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Vascular endothelial dysfunction (VED) plays an important role in the initiation of cardiovascular diseases. Roselle, enriched with antioxidants, demonstrates high potential in alleviating hypertension. This study was undertaken to investigate the effects of roselle supplementation of VED and remodelling in a rodent model with prolonged nicotine administration. Male Sprague-Dawley rats (n = 6 per group) were administered with 0.6 mg/kg nicotine for 28 days to induce VED. The rats were given either aqueous roselle (100 mg/kg) or normal saline orally 30 min prior to nicotine injection daily. One additional group of rats served as control. Thoracic aorta was isolated from rats to measure vascular reactivity, vascular remodelling and oxidative stress. Roselle significantly lowered aortic sensitivity to phenylephrine-induced vasoconstriction (Endo-(+) Cmax = 234.5 ± 3.9%, Endo-(-) Cmax = 247.6 ± 5.2%) compared with untreated nicotine group (Endo-(+) Cmax = 264.5 ± 6.9%, Endo-(-) Cmax = 276.5 ± 6.8%). Roselle also improved aortic response to endothelium-dependent vasodilator, acetylcholine (Endo-(+) Rmax = 73.2 ± 2.1%, Endo-(-) Rmax = 26.2 ± 0.8%) compared to nicotine group (Endo-(+) Rmax = 57.8 ± 1.7%, Endo-(-) Rmax = 20.9 ± 0.8%). In addition, roselle prevented an increase in intimal media thickness and elastic lamellae proliferation to preserve vascular architecture. Moreover, we also observed a significantly lowered degree of oxidative stress in parallel with increased antioxidant enzymes in aortic tissues of the roselle-treated group. This study demonstrated that roselle prevents VED and remodelling, and as such it has high nutraceutical value as supplement to prevent cardiovascular diseases.

Original languageEnglish
Pages (from-to)765-772
Number of pages8
JournalApplied Physiology, Nutrition and Metabolism
Volume42
Issue number7
DOIs
Publication statusPublished - 2017

Fingerprint

Hibiscus
Nicotine
Blood Vessels
Oxidative Stress
Cardiovascular Diseases
Antioxidants
Tunica Intima
Endothelium-Dependent Relaxing Factors
Phenylephrine
Dietary Supplements
Vasoconstriction
Thoracic Aorta
Acetylcholine
Sprague Dawley Rats
Rodentia
Hypertension
Injections

Keywords

  • Endothelial dysfunction
  • Hypertension
  • Nicotine
  • Oxidative stress
  • Roselle
  • Vascular remodelling

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Nutrition and Dietetics
  • Physiology (medical)

Cite this

Roselle supplementation prevents nicotine-induced vascular endothelial dysfunction and remodelling in rats. / Si, Lislivia Yiang Nee; Yusof, Kamisah; Ramalingam, Anand; Lim, Yi Cheng; Budin, Siti Balkis; Zainalabidin, Satirah.

In: Applied Physiology, Nutrition and Metabolism, Vol. 42, No. 7, 2017, p. 765-772.

Research output: Contribution to journalArticle

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abstract = "Vascular endothelial dysfunction (VED) plays an important role in the initiation of cardiovascular diseases. Roselle, enriched with antioxidants, demonstrates high potential in alleviating hypertension. This study was undertaken to investigate the effects of roselle supplementation of VED and remodelling in a rodent model with prolonged nicotine administration. Male Sprague-Dawley rats (n = 6 per group) were administered with 0.6 mg/kg nicotine for 28 days to induce VED. The rats were given either aqueous roselle (100 mg/kg) or normal saline orally 30 min prior to nicotine injection daily. One additional group of rats served as control. Thoracic aorta was isolated from rats to measure vascular reactivity, vascular remodelling and oxidative stress. Roselle significantly lowered aortic sensitivity to phenylephrine-induced vasoconstriction (Endo-(+) Cmax = 234.5 ± 3.9{\%}, Endo-(-) Cmax = 247.6 ± 5.2{\%}) compared with untreated nicotine group (Endo-(+) Cmax = 264.5 ± 6.9{\%}, Endo-(-) Cmax = 276.5 ± 6.8{\%}). Roselle also improved aortic response to endothelium-dependent vasodilator, acetylcholine (Endo-(+) Rmax = 73.2 ± 2.1{\%}, Endo-(-) Rmax = 26.2 ± 0.8{\%}) compared to nicotine group (Endo-(+) Rmax = 57.8 ± 1.7{\%}, Endo-(-) Rmax = 20.9 ± 0.8{\%}). In addition, roselle prevented an increase in intimal media thickness and elastic lamellae proliferation to preserve vascular architecture. Moreover, we also observed a significantly lowered degree of oxidative stress in parallel with increased antioxidant enzymes in aortic tissues of the roselle-treated group. This study demonstrated that roselle prevents VED and remodelling, and as such it has high nutraceutical value as supplement to prevent cardiovascular diseases.",
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AU - Si, Lislivia Yiang Nee

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AU - Zainalabidin, Satirah

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