Roselle attenuates cardiac hypertrophy after myocardial infarction in vivo and in vitro

Si Lislivia-Yiang-Nee, Anand Ramalingam, Shafreena Shaukat Ali, Amnani Aminuddin, Ng Pei-Yuen, Jalifah Latip, Yusof Kamisah, Siti Balkis Budin, Satirah Zainalabidin

Research output: Contribution to journalArticle

Abstract

Roselle (Hibiscus sabdariffa Linn) has been traditionally used as folk medicine for hypertension and maintaining cardiovascular health, with therapeutic potential in protecting against numerous cardiovascular diseases. However, it remains unclear whether roselle can be used for management of cardiac hypertrophy seen after myocardial infarction (MI). This study therefore investigated the effects of aqueous roselle extract on cardiac hypertrophy arising from myocardial infarction both in vivo and in vitro. For in vivo study, male Sprague-Dawley rats were divided into control or MI groups (receiving 85 mg/kg isoproterenol s.c. for 2 days) and were given roselle extract (100 mg/kg, p.o daily) for 28 days. Cardiac structure and functional changes were evaluated at study end-point using histology, Langendorff analysis and gene expression analysis. In vitro effects of roselle were also assessed on ANG II-induced cardiomyocytes hypertrophy using H9c2 cells, simulating cardiac hypertrophy evident after MI. Roselle significantly ameliorated MI-induced cardiac systolic and diastolic dysfunction, as seen across improvement in left ventricular developed pressure (LVDP) and its derivative (LVdP/dtmax) and isovolumic relaxation (Tau). Oxidative stress evident across elevated pro-oxidant markers (NOX2 subunit of NADPH oxidase and 8- isoprostane) as well as reduced antioxidant markers (superoxide dismutase and glutathione) were also significantly attenuated by roselle. Furthermore, roselle treatment markedly reduced markers of cardiac remodeling (cardiac hypertrophy and fibrosis) compared to the untreated MI rats. On in vitro analysis, roselle significantly attenuated ANG II-induced cardiomyoycte hypertrophy in dose-dependent manner. This study demonstrated that roselle attenuates cardiac hypertrophy and dysfunction seen after MI both in vivo and in vitro, and these effects are likely mediated by phenolic compounds found in roselle extract.

Original languageEnglish
Pages (from-to)876-892
Number of pages17
JournalEXCLI Journal
Volume18
DOIs
Publication statusPublished - 1 Jan 2019

Fingerprint

Hibiscus
Hibiscus sabdariffa
myocardial infarction
Cardiomegaly
hypertrophy
Myocardial Infarction
8-epi-prostaglandin F2alpha
Hypertrophy
In Vitro Techniques
extracts
NADPH Oxidase
rats
Traditional Medicine
Ventricular Pressure
in vivo studies
traditional medicine
fibrosis
Isoproterenol
Cardiac Myocytes
oxidants

Keywords

  • Cardiac dysfunction
  • Cardiomyocyte hypertrophy
  • Fibrosis
  • Myocardial infarction
  • Oxidative stress
  • Roselle

ASJC Scopus subject areas

  • Molecular Medicine
  • Animal Science and Zoology
  • Pharmacology
  • Drug Discovery

Cite this

Roselle attenuates cardiac hypertrophy after myocardial infarction in vivo and in vitro. / Lislivia-Yiang-Nee, Si; Ramalingam, Anand; Shaukat Ali, Shafreena; Aminuddin, Amnani; Pei-Yuen, Ng; Latip, Jalifah; Kamisah, Yusof; Balkis Budin, Siti; Zainalabidin, Satirah.

In: EXCLI Journal, Vol. 18, 01.01.2019, p. 876-892.

Research output: Contribution to journalArticle

Lislivia-Yiang-Nee, S, Ramalingam, A, Shaukat Ali, S, Aminuddin, A, Pei-Yuen, N, Latip, J, Kamisah, Y, Balkis Budin, S & Zainalabidin, S 2019, 'Roselle attenuates cardiac hypertrophy after myocardial infarction in vivo and in vitro', EXCLI Journal, vol. 18, pp. 876-892. https://doi.org/10.17179/excli2019-1792
Lislivia-Yiang-Nee S, Ramalingam A, Shaukat Ali S, Aminuddin A, Pei-Yuen N, Latip J et al. Roselle attenuates cardiac hypertrophy after myocardial infarction in vivo and in vitro. EXCLI Journal. 2019 Jan 1;18:876-892. https://doi.org/10.17179/excli2019-1792
Lislivia-Yiang-Nee, Si ; Ramalingam, Anand ; Shaukat Ali, Shafreena ; Aminuddin, Amnani ; Pei-Yuen, Ng ; Latip, Jalifah ; Kamisah, Yusof ; Balkis Budin, Siti ; Zainalabidin, Satirah. / Roselle attenuates cardiac hypertrophy after myocardial infarction in vivo and in vitro. In: EXCLI Journal. 2019 ; Vol. 18. pp. 876-892.
@article{e57726b1bd294fca9404da829870ae45,
title = "Roselle attenuates cardiac hypertrophy after myocardial infarction in vivo and in vitro",
abstract = "Roselle (Hibiscus sabdariffa Linn) has been traditionally used as folk medicine for hypertension and maintaining cardiovascular health, with therapeutic potential in protecting against numerous cardiovascular diseases. However, it remains unclear whether roselle can be used for management of cardiac hypertrophy seen after myocardial infarction (MI). This study therefore investigated the effects of aqueous roselle extract on cardiac hypertrophy arising from myocardial infarction both in vivo and in vitro. For in vivo study, male Sprague-Dawley rats were divided into control or MI groups (receiving 85 mg/kg isoproterenol s.c. for 2 days) and were given roselle extract (100 mg/kg, p.o daily) for 28 days. Cardiac structure and functional changes were evaluated at study end-point using histology, Langendorff analysis and gene expression analysis. In vitro effects of roselle were also assessed on ANG II-induced cardiomyocytes hypertrophy using H9c2 cells, simulating cardiac hypertrophy evident after MI. Roselle significantly ameliorated MI-induced cardiac systolic and diastolic dysfunction, as seen across improvement in left ventricular developed pressure (LVDP) and its derivative (LVdP/dtmax) and isovolumic relaxation (Tau). Oxidative stress evident across elevated pro-oxidant markers (NOX2 subunit of NADPH oxidase and 8- isoprostane) as well as reduced antioxidant markers (superoxide dismutase and glutathione) were also significantly attenuated by roselle. Furthermore, roselle treatment markedly reduced markers of cardiac remodeling (cardiac hypertrophy and fibrosis) compared to the untreated MI rats. On in vitro analysis, roselle significantly attenuated ANG II-induced cardiomyoycte hypertrophy in dose-dependent manner. This study demonstrated that roselle attenuates cardiac hypertrophy and dysfunction seen after MI both in vivo and in vitro, and these effects are likely mediated by phenolic compounds found in roselle extract.",
keywords = "Cardiac dysfunction, Cardiomyocyte hypertrophy, Fibrosis, Myocardial infarction, Oxidative stress, Roselle",
author = "Si Lislivia-Yiang-Nee and Anand Ramalingam and {Shaukat Ali}, Shafreena and Amnani Aminuddin and Ng Pei-Yuen and Jalifah Latip and Yusof Kamisah and {Balkis Budin}, Siti and Satirah Zainalabidin",
year = "2019",
month = "1",
day = "1",
doi = "10.17179/excli2019-1792",
language = "English",
volume = "18",
pages = "876--892",
journal = "EXCLI Journal",
issn = "1611-2156",
publisher = "University of Mainz",

}

TY - JOUR

T1 - Roselle attenuates cardiac hypertrophy after myocardial infarction in vivo and in vitro

AU - Lislivia-Yiang-Nee, Si

AU - Ramalingam, Anand

AU - Shaukat Ali, Shafreena

AU - Aminuddin, Amnani

AU - Pei-Yuen, Ng

AU - Latip, Jalifah

AU - Kamisah, Yusof

AU - Balkis Budin, Siti

AU - Zainalabidin, Satirah

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Roselle (Hibiscus sabdariffa Linn) has been traditionally used as folk medicine for hypertension and maintaining cardiovascular health, with therapeutic potential in protecting against numerous cardiovascular diseases. However, it remains unclear whether roselle can be used for management of cardiac hypertrophy seen after myocardial infarction (MI). This study therefore investigated the effects of aqueous roselle extract on cardiac hypertrophy arising from myocardial infarction both in vivo and in vitro. For in vivo study, male Sprague-Dawley rats were divided into control or MI groups (receiving 85 mg/kg isoproterenol s.c. for 2 days) and were given roselle extract (100 mg/kg, p.o daily) for 28 days. Cardiac structure and functional changes were evaluated at study end-point using histology, Langendorff analysis and gene expression analysis. In vitro effects of roselle were also assessed on ANG II-induced cardiomyocytes hypertrophy using H9c2 cells, simulating cardiac hypertrophy evident after MI. Roselle significantly ameliorated MI-induced cardiac systolic and diastolic dysfunction, as seen across improvement in left ventricular developed pressure (LVDP) and its derivative (LVdP/dtmax) and isovolumic relaxation (Tau). Oxidative stress evident across elevated pro-oxidant markers (NOX2 subunit of NADPH oxidase and 8- isoprostane) as well as reduced antioxidant markers (superoxide dismutase and glutathione) were also significantly attenuated by roselle. Furthermore, roselle treatment markedly reduced markers of cardiac remodeling (cardiac hypertrophy and fibrosis) compared to the untreated MI rats. On in vitro analysis, roselle significantly attenuated ANG II-induced cardiomyoycte hypertrophy in dose-dependent manner. This study demonstrated that roselle attenuates cardiac hypertrophy and dysfunction seen after MI both in vivo and in vitro, and these effects are likely mediated by phenolic compounds found in roselle extract.

AB - Roselle (Hibiscus sabdariffa Linn) has been traditionally used as folk medicine for hypertension and maintaining cardiovascular health, with therapeutic potential in protecting against numerous cardiovascular diseases. However, it remains unclear whether roselle can be used for management of cardiac hypertrophy seen after myocardial infarction (MI). This study therefore investigated the effects of aqueous roselle extract on cardiac hypertrophy arising from myocardial infarction both in vivo and in vitro. For in vivo study, male Sprague-Dawley rats were divided into control or MI groups (receiving 85 mg/kg isoproterenol s.c. for 2 days) and were given roselle extract (100 mg/kg, p.o daily) for 28 days. Cardiac structure and functional changes were evaluated at study end-point using histology, Langendorff analysis and gene expression analysis. In vitro effects of roselle were also assessed on ANG II-induced cardiomyocytes hypertrophy using H9c2 cells, simulating cardiac hypertrophy evident after MI. Roselle significantly ameliorated MI-induced cardiac systolic and diastolic dysfunction, as seen across improvement in left ventricular developed pressure (LVDP) and its derivative (LVdP/dtmax) and isovolumic relaxation (Tau). Oxidative stress evident across elevated pro-oxidant markers (NOX2 subunit of NADPH oxidase and 8- isoprostane) as well as reduced antioxidant markers (superoxide dismutase and glutathione) were also significantly attenuated by roselle. Furthermore, roselle treatment markedly reduced markers of cardiac remodeling (cardiac hypertrophy and fibrosis) compared to the untreated MI rats. On in vitro analysis, roselle significantly attenuated ANG II-induced cardiomyoycte hypertrophy in dose-dependent manner. This study demonstrated that roselle attenuates cardiac hypertrophy and dysfunction seen after MI both in vivo and in vitro, and these effects are likely mediated by phenolic compounds found in roselle extract.

KW - Cardiac dysfunction

KW - Cardiomyocyte hypertrophy

KW - Fibrosis

KW - Myocardial infarction

KW - Oxidative stress

KW - Roselle

UR - http://www.scopus.com/inward/record.url?scp=85073454491&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85073454491&partnerID=8YFLogxK

U2 - 10.17179/excli2019-1792

DO - 10.17179/excli2019-1792

M3 - Article

AN - SCOPUS:85073454491

VL - 18

SP - 876

EP - 892

JO - EXCLI Journal

JF - EXCLI Journal

SN - 1611-2156

ER -