Role of the cell envelope in the antibacterial activities of polymyxin B and polymyxin B nonapeptide against Escherichia coli

Ahmad Zorin Sahalan, Ronald A. Dixon

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The role of membrane permeabilisation and disruption in the mechanism of action of some polymyxin analogues against Gram-negative organisms is contentious. The effects of polymyxin B (PMB) and its analogue polymyxin B nonapeptide (PMBN) on Escherichia coli envelopes should correlate, but previous work by other workers suggests that PMBN has a different mode of action. This study has reassessed the biochemical techniques used previously and has shown that, in contrast to previous studies, PMBN (a well-characterised antibacterial synergist) readily releases periplasmic proteins and lipopolysaccharide from treated E. coli at subinhibitory concentrations in normal physiological buffer conditions. We conclude that, when tested with appropriate methodology, PMBN closely correlates with the early effects of PMB on the cell envelope of E. coli and this study shows that it is now consistent with the accepted interactions of membrane-active agents against Gram-negative cells.

Original languageEnglish
Pages (from-to)224-227
Number of pages4
JournalInternational Journal of Antimicrobial Agents
Volume31
Issue number3
DOIs
Publication statusPublished - Mar 2008

Fingerprint

Polymyxin B
Escherichia coli
Periplasmic Proteins
Polymyxins
Membranes
Lipopolysaccharides
Buffers
polymyxin B nonapeptide

Keywords

  • Bacterial membranes
  • Escherichia coli
  • Mode of action
  • Polymyxin
  • Polymyxin nonapeptide

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Microbiology
  • Parasitology
  • Virology
  • Immunology and Allergy
  • Infectious Diseases

Cite this

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