Role of rutin on nitric oxide synthesis in human umbilical vein endothelial cells

Azizah Ugusman, Zaiton Zakaria, Chua Kien Hui, Nor Anita Megat Mohd Nordin, Zaleha Abdullah Mahdy

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Nitric oxide (NO), produced by endothelial nitric oxide synthase (eNOS), is a major antiatherogenic factor in the blood vessel. Oxidative stress plays an important role in the pathogenesis of various cardiovascular diseases, including atherosclerosis. Decreased availability of endothelial NO promotes the progression of endothelial dysfunction and atherosclerosis. Rutin is a flavonoid with multiple cardiovascular protective effects. This study aimed to investigate the effects of rutin on eNOS and NO production in cultured human umbilical vein endothelial cells (HUVEC). HUVEC were divided into four groups: control; oxidative stress induction with 180 M H2O2; treatment with 300 M rutin; and concomitant induction with rutin and H 2O2 for 24 hours. HUVEC treated with rutin produced higher amount of NO compared to control (P < 0.01). In the oxidative stress-induced HUVEC, rutin successfully induced cells' NO production (P < 0.01). Rutin promoted NO production in HUVEC by inducing eNOS gene expression (P < 0.05), eNOS protein synthesis (P < 0.01), and eNOS activity (P < 0.05). Treatment with rutin also led to increased gene and protein expression of basic fibroblast growth factor (bFGF) in HUVEC. Therefore, upregulation of eNOS expression by rutin may be mediated by bFGF. The results showed that rutin may improve endothelial function by augmenting NO production in human endothelial cells.

Original languageEnglish
Article number169370
JournalScientific World Journal
Volume2014
DOIs
Publication statusPublished - 2014

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Rutin
Endothelial cells
Human Umbilical Vein Endothelial Cells
nitric oxide
Nitric Oxide
Nitric Oxide Synthase Type III
Oxidative stress
Oxidative Stress
Fibroblast Growth Factor 2
Atherosclerosis
Gene Expression
Blood vessels
Flavonoids
Gene expression
protein
Blood Vessels
cardiovascular disease
Proteins
Cardiovascular Diseases
Up-Regulation

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Medicine(all)

Cite this

Role of rutin on nitric oxide synthesis in human umbilical vein endothelial cells. / Ugusman, Azizah; Zakaria, Zaiton; Kien Hui, Chua; Megat Mohd Nordin, Nor Anita; Abdullah Mahdy, Zaleha.

In: Scientific World Journal, Vol. 2014, 169370, 2014.

Research output: Contribution to journalArticle

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AU - Abdullah Mahdy, Zaleha

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