Role of plasma-derived fibrin on keratinocyte and fibroblast wound healing

Jia Xian Law, Shiplu Roy Chowdhury, Bin Saim Aminuddin, Ruszymah Idrus

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Fibrin has excellent biocompatibility and biological properties to support tissue regeneration and promote wound healing. However, the role of diluted fibrin in wound healing has yet to be elucidated as it is commonly used in high concentration. This study was aimed to examine the effects of diluted plasma-derived fibrin (PDF) on keratinocyte and fibroblast wound healing in term of cell proliferation, migration, extracellular matrix (ECM) production and soluble factor secretion. Two PDF concentrations, 10 and 20% (v/v) were tested on keratinocytes and fibroblasts indirectly co-cultured in the transwell system. The control group was cultured with 5% FBS. Results showed that PDF reduced the keratinocyte growth rate and fibroblast migration, and increased the fibroblast ECM gene expression whereby significant differences were found between the 20% PDF group and the 5% FBS group. Similar trend was seen for the 10% PDF group but the differences were not significant. Comparison of the soluble factors between the PDF groups demonstrated that the level of growth-related oncogene alpha, interleukin-8 and epithelial neutrophil-activating peptide-78 were significantly higher in the 10% PDF group, whilst interleukin-1 alpha and granulocyte–macrophage colony stimulating factor were significantly more concentrated in the 20% PDF group. Our results suggested that PDF selectively elevated the expression of collagen type 1 and collagen type 3 in fibroblasts but slowed down the migration in concentration-dependent manner. These novel findings provide new insight into the role of PDF in wound healing and may have important implications for the use of fibrin in skin tissue engineering.

Original languageEnglish
Pages (from-to)1-11
Number of pages11
JournalCell and Tissue Banking
DOIs
Publication statusAccepted/In press - 26 Jul 2017

Fingerprint

Fibroblasts
Fibrin
Keratinocytes
Wound Healing
Plasmas
Collagen
Extracellular Matrix
Tissue regeneration
Cell proliferation
Colony-Stimulating Factors
Biocompatibility
Tissue engineering
Gene expression
Interleukin-1alpha
Peptides
Collagen Type III
Skin
Tissue Engineering
Growth
Collagen Type I

Keywords

  • Cell migration
  • Collagen
  • Fibrin
  • Fibroblast
  • Keratinocyte
  • Wound healing

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Cell Biology
  • Transplantation

Cite this

Role of plasma-derived fibrin on keratinocyte and fibroblast wound healing. / Law, Jia Xian; Chowdhury, Shiplu Roy; Aminuddin, Bin Saim; Idrus, Ruszymah.

In: Cell and Tissue Banking, 26.07.2017, p. 1-11.

Research output: Contribution to journalArticle

@article{3579a48803a8454d8b806169cb3dfbad,
title = "Role of plasma-derived fibrin on keratinocyte and fibroblast wound healing",
abstract = "Fibrin has excellent biocompatibility and biological properties to support tissue regeneration and promote wound healing. However, the role of diluted fibrin in wound healing has yet to be elucidated as it is commonly used in high concentration. This study was aimed to examine the effects of diluted plasma-derived fibrin (PDF) on keratinocyte and fibroblast wound healing in term of cell proliferation, migration, extracellular matrix (ECM) production and soluble factor secretion. Two PDF concentrations, 10 and 20{\%} (v/v) were tested on keratinocytes and fibroblasts indirectly co-cultured in the transwell system. The control group was cultured with 5{\%} FBS. Results showed that PDF reduced the keratinocyte growth rate and fibroblast migration, and increased the fibroblast ECM gene expression whereby significant differences were found between the 20{\%} PDF group and the 5{\%} FBS group. Similar trend was seen for the 10{\%} PDF group but the differences were not significant. Comparison of the soluble factors between the PDF groups demonstrated that the level of growth-related oncogene alpha, interleukin-8 and epithelial neutrophil-activating peptide-78 were significantly higher in the 10{\%} PDF group, whilst interleukin-1 alpha and granulocyte–macrophage colony stimulating factor were significantly more concentrated in the 20{\%} PDF group. Our results suggested that PDF selectively elevated the expression of collagen type 1 and collagen type 3 in fibroblasts but slowed down the migration in concentration-dependent manner. These novel findings provide new insight into the role of PDF in wound healing and may have important implications for the use of fibrin in skin tissue engineering.",
keywords = "Cell migration, Collagen, Fibrin, Fibroblast, Keratinocyte, Wound healing",
author = "Law, {Jia Xian} and Chowdhury, {Shiplu Roy} and Aminuddin, {Bin Saim} and Ruszymah Idrus",
year = "2017",
month = "7",
day = "26",
doi = "10.1007/s10561-017-9645-2",
language = "English",
pages = "1--11",
journal = "Cell and Tissue Banking",
issn = "1389-9333",
publisher = "Springer Netherlands",

}

TY - JOUR

T1 - Role of plasma-derived fibrin on keratinocyte and fibroblast wound healing

AU - Law, Jia Xian

AU - Chowdhury, Shiplu Roy

AU - Aminuddin, Bin Saim

AU - Idrus, Ruszymah

PY - 2017/7/26

Y1 - 2017/7/26

N2 - Fibrin has excellent biocompatibility and biological properties to support tissue regeneration and promote wound healing. However, the role of diluted fibrin in wound healing has yet to be elucidated as it is commonly used in high concentration. This study was aimed to examine the effects of diluted plasma-derived fibrin (PDF) on keratinocyte and fibroblast wound healing in term of cell proliferation, migration, extracellular matrix (ECM) production and soluble factor secretion. Two PDF concentrations, 10 and 20% (v/v) were tested on keratinocytes and fibroblasts indirectly co-cultured in the transwell system. The control group was cultured with 5% FBS. Results showed that PDF reduced the keratinocyte growth rate and fibroblast migration, and increased the fibroblast ECM gene expression whereby significant differences were found between the 20% PDF group and the 5% FBS group. Similar trend was seen for the 10% PDF group but the differences were not significant. Comparison of the soluble factors between the PDF groups demonstrated that the level of growth-related oncogene alpha, interleukin-8 and epithelial neutrophil-activating peptide-78 were significantly higher in the 10% PDF group, whilst interleukin-1 alpha and granulocyte–macrophage colony stimulating factor were significantly more concentrated in the 20% PDF group. Our results suggested that PDF selectively elevated the expression of collagen type 1 and collagen type 3 in fibroblasts but slowed down the migration in concentration-dependent manner. These novel findings provide new insight into the role of PDF in wound healing and may have important implications for the use of fibrin in skin tissue engineering.

AB - Fibrin has excellent biocompatibility and biological properties to support tissue regeneration and promote wound healing. However, the role of diluted fibrin in wound healing has yet to be elucidated as it is commonly used in high concentration. This study was aimed to examine the effects of diluted plasma-derived fibrin (PDF) on keratinocyte and fibroblast wound healing in term of cell proliferation, migration, extracellular matrix (ECM) production and soluble factor secretion. Two PDF concentrations, 10 and 20% (v/v) were tested on keratinocytes and fibroblasts indirectly co-cultured in the transwell system. The control group was cultured with 5% FBS. Results showed that PDF reduced the keratinocyte growth rate and fibroblast migration, and increased the fibroblast ECM gene expression whereby significant differences were found between the 20% PDF group and the 5% FBS group. Similar trend was seen for the 10% PDF group but the differences were not significant. Comparison of the soluble factors between the PDF groups demonstrated that the level of growth-related oncogene alpha, interleukin-8 and epithelial neutrophil-activating peptide-78 were significantly higher in the 10% PDF group, whilst interleukin-1 alpha and granulocyte–macrophage colony stimulating factor were significantly more concentrated in the 20% PDF group. Our results suggested that PDF selectively elevated the expression of collagen type 1 and collagen type 3 in fibroblasts but slowed down the migration in concentration-dependent manner. These novel findings provide new insight into the role of PDF in wound healing and may have important implications for the use of fibrin in skin tissue engineering.

KW - Cell migration

KW - Collagen

KW - Fibrin

KW - Fibroblast

KW - Keratinocyte

KW - Wound healing

UR - http://www.scopus.com/inward/record.url?scp=85026914451&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85026914451&partnerID=8YFLogxK

U2 - 10.1007/s10561-017-9645-2

DO - 10.1007/s10561-017-9645-2

M3 - Article

C2 - 28748415

AN - SCOPUS:85026914451

SP - 1

EP - 11

JO - Cell and Tissue Banking

JF - Cell and Tissue Banking

SN - 1389-9333

ER -