Role of horseradish peroxidase in polypyrrole conductivity

Siti Amira Othman, Shahidan Radiman

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Immobilise enzymes can offer many advantages over their soluble forms making this is a topic of active research in enzyme technology for industrial applications. There are various methods for enzyme immobilization as well as support materials. The methods and supports used for immobilisation of certain enzyme are chosen to ensure the highest retention of activity and its stability. Enzyme Horseradish peroxidise (HRP) was used extensively in molecular biology applications primarily for its ability to amplify a weak signal and increase the detectability of a target molecules. Recently class of conducting polymers are used as a polymeric support. Among the family a conducting polymer, polypyrrole (PPY) has intensively been used for various reason because of its unique properties such as ease of synthesis by chemical or electrochemical oxidative polymerization of the monomers, role as a polymeric support and also have potential in various field. It has alternating single and double carbon-carbon bonds along the polymeric chains. The highly conjugated polymer chain can be assigned reversible chemical, electrochemical and physical properties controlled by a doping/de-doping process, which makes this polymers very attractive as transducer materials in various sensing devices. This paper reports the process of immobilised enzymes on polymeric substrate and the role of enzymes in PPY conductivity. The characterization was done using UV-Visible, FTIR and four point probe.

Original languageEnglish
Title of host publicationSolid State Science and Technology XXIX - 29th RCSSST
PublisherTrans Tech Publications Ltd
Pages370-373
Number of pages4
Volume268 SSP
ISBN (Print)9783035711288
DOIs
Publication statusPublished - 2017
Event29th Regional Conference of Solid State Science and Technology, RCSSST 2016 - Johor Bahru, Malaysia
Duration: 15 Nov 201617 Nov 2016

Publication series

NameSolid State Phenomena
Volume268 SSP
ISSN (Electronic)1662-9779

Other

Other29th Regional Conference of Solid State Science and Technology, RCSSST 2016
CountryMalaysia
CityJohor Bahru
Period15/11/1617/11/16

Fingerprint

polypyrroles
Polypyrroles
Horseradish Peroxidase
enzymes
Enzymes
conductivity
Conducting polymers
Polymers
Carbon
conducting polymers
Doping (additives)
immobilization
Enzyme immobilization
Immobilized Enzymes
Molecular biology
polymers
Conjugated polymers
Electrochemical properties
molecular biology
Chemical properties

Keywords

  • Conductivity
  • Hrp
  • Polypyrrole

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Materials Science(all)
  • Condensed Matter Physics

Cite this

Othman, S. A., & Radiman, S. (2017). Role of horseradish peroxidase in polypyrrole conductivity. In Solid State Science and Technology XXIX - 29th RCSSST (Vol. 268 SSP, pp. 370-373). (Solid State Phenomena; Vol. 268 SSP). Trans Tech Publications Ltd. https://doi.org/10.4028/www.scientific.net/SSP.268.370

Role of horseradish peroxidase in polypyrrole conductivity. / Othman, Siti Amira; Radiman, Shahidan.

Solid State Science and Technology XXIX - 29th RCSSST. Vol. 268 SSP Trans Tech Publications Ltd, 2017. p. 370-373 (Solid State Phenomena; Vol. 268 SSP).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Othman, SA & Radiman, S 2017, Role of horseradish peroxidase in polypyrrole conductivity. in Solid State Science and Technology XXIX - 29th RCSSST. vol. 268 SSP, Solid State Phenomena, vol. 268 SSP, Trans Tech Publications Ltd, pp. 370-373, 29th Regional Conference of Solid State Science and Technology, RCSSST 2016, Johor Bahru, Malaysia, 15/11/16. https://doi.org/10.4028/www.scientific.net/SSP.268.370
Othman SA, Radiman S. Role of horseradish peroxidase in polypyrrole conductivity. In Solid State Science and Technology XXIX - 29th RCSSST. Vol. 268 SSP. Trans Tech Publications Ltd. 2017. p. 370-373. (Solid State Phenomena). https://doi.org/10.4028/www.scientific.net/SSP.268.370
Othman, Siti Amira ; Radiman, Shahidan. / Role of horseradish peroxidase in polypyrrole conductivity. Solid State Science and Technology XXIX - 29th RCSSST. Vol. 268 SSP Trans Tech Publications Ltd, 2017. pp. 370-373 (Solid State Phenomena).
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