Reversal of myoblast aging by tocotrienol rich fraction posttreatment

Jing Jye Lim, Wan Zurinah Wan Ngah, Vincent Mouly, Norwahidah Abdul Karim

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Skeletal muscle satellite cells are heavily involved in the regeneration of skeletal muscle in response to the aging-related deterioration of the skeletal muscle mass, strength, and regenerative capacity, termed as sarcopenia. This study focused on the effect of tocotrienol rich fraction (TRF) on regenerative capacity of myoblasts in stress-induced premature senescence (SIPS). The myoblasts was grouped as young control, SIPS-induced, TRF control, TRF pretreatment, and TRF posttreatment. Optimum dose of TRF, morphological observation, activity of senescence-associated β-galactosidase (SA-β-galactosidase), and cell proliferation were determined. 50 g/mL TRF treatment exhibited the highest cell proliferation capacity. SIPS-induced myoblasts exhibit large flattened cells and prominent intermediate filaments (senescent-like morphology). The activity of SA-β-galactosidase was significantly increased, but the proliferation capacity was significantly reduced as compared to young control. The activity of SA-β-galactosidase was significantly reduced and cell proliferation was significantly increased in the posttreatment group whereas there was no significant difference in SA-β-galactosidase activity and proliferation capacity of pretreatment group as compared to SIPS-induced myoblasts. Based on the data, we hypothesized that TRF may reverse the myoblasts aging through replenishing the regenerative capacity of the cells. However, further investigation on the mechanism of TRF in reversing the myoblast aging is needed.

Original languageEnglish
Article number978101
JournalOxidative Medicine and Cellular Longevity
DOIs
Publication statusPublished - 2013

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Tocotrienols
Myoblasts
Aging of materials
Galactosidases
Cell proliferation
Muscle
Cell Proliferation
Skeletal Muscle
Skeletal Muscle Satellite Cells
Sarcopenia
Intermediate Filaments
Muscle Strength
Deterioration
Regeneration
Observation
Satellites

ASJC Scopus subject areas

  • Cell Biology
  • Ageing
  • Biochemistry

Cite this

Reversal of myoblast aging by tocotrienol rich fraction posttreatment. / Lim, Jing Jye; Wan Ngah, Wan Zurinah; Mouly, Vincent; Abdul Karim, Norwahidah.

In: Oxidative Medicine and Cellular Longevity, 2013.

Research output: Contribution to journalArticle

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