Retinal degeneration rat model: A study on the structural and functional changes in the retina following injection of sodium iodate

Avin Ee Hwan Koh, Hiba Amer Alsaeedi, Munirah binti Abd Rashid, Chenshen Lam, Mohd Hairul Nizam Harun, Muhamad Fakhri bin Mohd Saleh, Chi D. Luu, S. Suresh Kumar, Min Hwei Ng, Hazlita Mohd Isa, Sue Ngein Leow, Kong Yong Then, Mae-Lynn Catherine Bastion, Mohammed Safwan Ali Khan, Pooi Ling Mok

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Retinal disorders account for a large proportion of ocular disorders that can lead to visual impairment or blindness, and yet our limited knowledge in the pathogenesis and choice of appropriate animal models for new treatment modalities may contribute to ineffective therapies. Although genetic in vivo models are favored, the variable expressivity and penetrance of these heterogeneous disorders can cause difficulties in assessing potential treatments against retinal degeneration. Hence, an attractive alternative is to develop a chemically-induced model that is both cost-friendly and standardizable. Sodium iodate is an oxidative chemical that is used to simulate late stage retinitis pigmentosa and age-related macular degeneration. In this study, retinal degeneration was induced through systemic administration of sodium iodate (NaIO3) at varying doses up to 80 mg/kg in Sprague-Dawley rats. An analysis on the visual response of the rats by electroretinography (ERG) showed a decrease in photoreceptor function with NaIO3 administration at a dose of 40 mg/kg or greater. The results correlated with the TUNEL assay, which revealed signs of DNA damage throughout the retina. Histomorphological analysis also revealed extensive structural lesions throughout the outer retina and parts of the inner retina. Our results provided a detailed view of NaIO3-induced retinal degeneration, and showed that the administration of 40 mg/kg NaIO3 was sufficient to generate disturbances in retinal function. The pathological findings in this model reveal a degenerating retina, and can be further utilized to develop effective therapies for RPE, photoreceptor, and bipolar cell regeneration.

Original languageEnglish
Article number111514
JournalJournal of Photochemistry and Photobiology B: Biology
Volume196
DOIs
Publication statusPublished - 1 Jul 2019

Fingerprint

iodates
Retinal Degeneration
degeneration
retina
rats
Retina
sodium
injection
photoreceptors
Injections
disorders
electroretinography
therapy
blindness
pathogenesis
dosage
animal models
impairment
regeneration
lesions

Keywords

  • Electroretinography
  • Photoreceptors
  • Retinal degeneration
  • Retinal pigment epithelium
  • Sodium iodate

ASJC Scopus subject areas

  • Radiation
  • Radiological and Ultrasound Technology
  • Biophysics
  • Radiology Nuclear Medicine and imaging

Cite this

Retinal degeneration rat model : A study on the structural and functional changes in the retina following injection of sodium iodate. / Koh, Avin Ee Hwan; Alsaeedi, Hiba Amer; Rashid, Munirah binti Abd; Lam, Chenshen; Harun, Mohd Hairul Nizam; Saleh, Muhamad Fakhri bin Mohd; Luu, Chi D.; Kumar, S. Suresh; Ng, Min Hwei; Mohd Isa, Hazlita; Leow, Sue Ngein; Then, Kong Yong; Bastion, Mae-Lynn Catherine; Ali Khan, Mohammed Safwan; Mok, Pooi Ling.

In: Journal of Photochemistry and Photobiology B: Biology, Vol. 196, 111514, 01.07.2019.

Research output: Contribution to journalArticle

Koh, AEH, Alsaeedi, HA, Rashid, MBA, Lam, C, Harun, MHN, Saleh, MFBM, Luu, CD, Kumar, SS, Ng, MH, Mohd Isa, H, Leow, SN, Then, KY, Bastion, M-LC, Ali Khan, MS & Mok, PL 2019, 'Retinal degeneration rat model: A study on the structural and functional changes in the retina following injection of sodium iodate', Journal of Photochemistry and Photobiology B: Biology, vol. 196, 111514. https://doi.org/10.1016/j.jphotobiol.2019.111514
Koh, Avin Ee Hwan ; Alsaeedi, Hiba Amer ; Rashid, Munirah binti Abd ; Lam, Chenshen ; Harun, Mohd Hairul Nizam ; Saleh, Muhamad Fakhri bin Mohd ; Luu, Chi D. ; Kumar, S. Suresh ; Ng, Min Hwei ; Mohd Isa, Hazlita ; Leow, Sue Ngein ; Then, Kong Yong ; Bastion, Mae-Lynn Catherine ; Ali Khan, Mohammed Safwan ; Mok, Pooi Ling. / Retinal degeneration rat model : A study on the structural and functional changes in the retina following injection of sodium iodate. In: Journal of Photochemistry and Photobiology B: Biology. 2019 ; Vol. 196.
@article{af97b924bd4447cba7e3000d6944e4da,
title = "Retinal degeneration rat model: A study on the structural and functional changes in the retina following injection of sodium iodate",
abstract = "Retinal disorders account for a large proportion of ocular disorders that can lead to visual impairment or blindness, and yet our limited knowledge in the pathogenesis and choice of appropriate animal models for new treatment modalities may contribute to ineffective therapies. Although genetic in vivo models are favored, the variable expressivity and penetrance of these heterogeneous disorders can cause difficulties in assessing potential treatments against retinal degeneration. Hence, an attractive alternative is to develop a chemically-induced model that is both cost-friendly and standardizable. Sodium iodate is an oxidative chemical that is used to simulate late stage retinitis pigmentosa and age-related macular degeneration. In this study, retinal degeneration was induced through systemic administration of sodium iodate (NaIO3) at varying doses up to 80 mg/kg in Sprague-Dawley rats. An analysis on the visual response of the rats by electroretinography (ERG) showed a decrease in photoreceptor function with NaIO3 administration at a dose of 40 mg/kg or greater. The results correlated with the TUNEL assay, which revealed signs of DNA damage throughout the retina. Histomorphological analysis also revealed extensive structural lesions throughout the outer retina and parts of the inner retina. Our results provided a detailed view of NaIO3-induced retinal degeneration, and showed that the administration of 40 mg/kg NaIO3 was sufficient to generate disturbances in retinal function. The pathological findings in this model reveal a degenerating retina, and can be further utilized to develop effective therapies for RPE, photoreceptor, and bipolar cell regeneration.",
keywords = "Electroretinography, Photoreceptors, Retinal degeneration, Retinal pigment epithelium, Sodium iodate",
author = "Koh, {Avin Ee Hwan} and Alsaeedi, {Hiba Amer} and Rashid, {Munirah binti Abd} and Chenshen Lam and Harun, {Mohd Hairul Nizam} and Saleh, {Muhamad Fakhri bin Mohd} and Luu, {Chi D.} and Kumar, {S. Suresh} and Ng, {Min Hwei} and {Mohd Isa}, Hazlita and Leow, {Sue Ngein} and Then, {Kong Yong} and Bastion, {Mae-Lynn Catherine} and {Ali Khan}, {Mohammed Safwan} and Mok, {Pooi Ling}",
year = "2019",
month = "7",
day = "1",
doi = "10.1016/j.jphotobiol.2019.111514",
language = "English",
volume = "196",
journal = "Journal of Photochemistry and Photobiology B: Biology",
issn = "1011-1344",
publisher = "Elsevier",

}

TY - JOUR

T1 - Retinal degeneration rat model

T2 - A study on the structural and functional changes in the retina following injection of sodium iodate

AU - Koh, Avin Ee Hwan

AU - Alsaeedi, Hiba Amer

AU - Rashid, Munirah binti Abd

AU - Lam, Chenshen

AU - Harun, Mohd Hairul Nizam

AU - Saleh, Muhamad Fakhri bin Mohd

AU - Luu, Chi D.

AU - Kumar, S. Suresh

AU - Ng, Min Hwei

AU - Mohd Isa, Hazlita

AU - Leow, Sue Ngein

AU - Then, Kong Yong

AU - Bastion, Mae-Lynn Catherine

AU - Ali Khan, Mohammed Safwan

AU - Mok, Pooi Ling

PY - 2019/7/1

Y1 - 2019/7/1

N2 - Retinal disorders account for a large proportion of ocular disorders that can lead to visual impairment or blindness, and yet our limited knowledge in the pathogenesis and choice of appropriate animal models for new treatment modalities may contribute to ineffective therapies. Although genetic in vivo models are favored, the variable expressivity and penetrance of these heterogeneous disorders can cause difficulties in assessing potential treatments against retinal degeneration. Hence, an attractive alternative is to develop a chemically-induced model that is both cost-friendly and standardizable. Sodium iodate is an oxidative chemical that is used to simulate late stage retinitis pigmentosa and age-related macular degeneration. In this study, retinal degeneration was induced through systemic administration of sodium iodate (NaIO3) at varying doses up to 80 mg/kg in Sprague-Dawley rats. An analysis on the visual response of the rats by electroretinography (ERG) showed a decrease in photoreceptor function with NaIO3 administration at a dose of 40 mg/kg or greater. The results correlated with the TUNEL assay, which revealed signs of DNA damage throughout the retina. Histomorphological analysis also revealed extensive structural lesions throughout the outer retina and parts of the inner retina. Our results provided a detailed view of NaIO3-induced retinal degeneration, and showed that the administration of 40 mg/kg NaIO3 was sufficient to generate disturbances in retinal function. The pathological findings in this model reveal a degenerating retina, and can be further utilized to develop effective therapies for RPE, photoreceptor, and bipolar cell regeneration.

AB - Retinal disorders account for a large proportion of ocular disorders that can lead to visual impairment or blindness, and yet our limited knowledge in the pathogenesis and choice of appropriate animal models for new treatment modalities may contribute to ineffective therapies. Although genetic in vivo models are favored, the variable expressivity and penetrance of these heterogeneous disorders can cause difficulties in assessing potential treatments against retinal degeneration. Hence, an attractive alternative is to develop a chemically-induced model that is both cost-friendly and standardizable. Sodium iodate is an oxidative chemical that is used to simulate late stage retinitis pigmentosa and age-related macular degeneration. In this study, retinal degeneration was induced through systemic administration of sodium iodate (NaIO3) at varying doses up to 80 mg/kg in Sprague-Dawley rats. An analysis on the visual response of the rats by electroretinography (ERG) showed a decrease in photoreceptor function with NaIO3 administration at a dose of 40 mg/kg or greater. The results correlated with the TUNEL assay, which revealed signs of DNA damage throughout the retina. Histomorphological analysis also revealed extensive structural lesions throughout the outer retina and parts of the inner retina. Our results provided a detailed view of NaIO3-induced retinal degeneration, and showed that the administration of 40 mg/kg NaIO3 was sufficient to generate disturbances in retinal function. The pathological findings in this model reveal a degenerating retina, and can be further utilized to develop effective therapies for RPE, photoreceptor, and bipolar cell regeneration.

KW - Electroretinography

KW - Photoreceptors

KW - Retinal degeneration

KW - Retinal pigment epithelium

KW - Sodium iodate

UR - http://www.scopus.com/inward/record.url?scp=85066868051&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85066868051&partnerID=8YFLogxK

U2 - 10.1016/j.jphotobiol.2019.111514

DO - 10.1016/j.jphotobiol.2019.111514

M3 - Article

C2 - 31154277

AN - SCOPUS:85066868051

VL - 196

JO - Journal of Photochemistry and Photobiology B: Biology

JF - Journal of Photochemistry and Photobiology B: Biology

SN - 1011-1344

M1 - 111514

ER -