Response surface optimization of culture medium for enhanced docosahexaenoic acid production by a Malaysian thraustochytrid

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Abstract

Docosahexaenoic acid (DHA, C22:6n-3) plays a vital role in the enhancement of human health, particularly for cognitive, neurological, and visual functions. Marine microalgae, such as members of the genus Aurantiochytrium, are rich in DHA and represent a promising source of omega-3 fatty acids. In this study, levels of glucose, yeast extract, sodium glutamate and sea salt were optimized for enhanced lipid and DHA production by a Malaysian isolate of thraustochytrid, Aurantiochytrium sp. SW1, using response surface methodology (RSM). The optimized medium contained 60g/L glucose, 2g/L yeast extract, 24g/L sodium glutamate and 6g/L sea salt. This combination produced 17.8g/L biomass containing 53.9% lipid (9.6g/L) which contained 44.07% DHA (4.23g/L). The optimized medium was used in a scale-up run, where a 5L bench-top bioreactor was employed to verify the applicability of the medium at larger scale. This produced 24.46g/L biomass containing 38.43% lipid (9.4g/L), of which 47.87% was DHA (4.5g/L). The total amount of DHA produced was 25% higher than that produced in the original medium prior to optimization. This result suggests that Aurantiochytrium sp. SW1 could be developed for industrial application as a commercial DHA-producing microorganism.

Original languageEnglish
Article number8611
JournalScientific Reports
Volume5
DOIs
Publication statusPublished - 2015

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Docosahexaenoic Acids
Culture Media
Sodium Glutamate
Lipids
Oceans and Seas
Biomass
Salts
Yeasts
Microalgae
Glucose
Omega-3 Fatty Acids
Bioreactors
Health

ASJC Scopus subject areas

  • General

Cite this

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title = "Response surface optimization of culture medium for enhanced docosahexaenoic acid production by a Malaysian thraustochytrid",
abstract = "Docosahexaenoic acid (DHA, C22:6n-3) plays a vital role in the enhancement of human health, particularly for cognitive, neurological, and visual functions. Marine microalgae, such as members of the genus Aurantiochytrium, are rich in DHA and represent a promising source of omega-3 fatty acids. In this study, levels of glucose, yeast extract, sodium glutamate and sea salt were optimized for enhanced lipid and DHA production by a Malaysian isolate of thraustochytrid, Aurantiochytrium sp. SW1, using response surface methodology (RSM). The optimized medium contained 60g/L glucose, 2g/L yeast extract, 24g/L sodium glutamate and 6g/L sea salt. This combination produced 17.8g/L biomass containing 53.9{\%} lipid (9.6g/L) which contained 44.07{\%} DHA (4.23g/L). The optimized medium was used in a scale-up run, where a 5L bench-top bioreactor was employed to verify the applicability of the medium at larger scale. This produced 24.46g/L biomass containing 38.43{\%} lipid (9.4g/L), of which 47.87{\%} was DHA (4.5g/L). The total amount of DHA produced was 25{\%} higher than that produced in the original medium prior to optimization. This result suggests that Aurantiochytrium sp. SW1 could be developed for industrial application as a commercial DHA-producing microorganism.",
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