Response surface optimisation of enzymatically hydrolysed and dilute acid pretreated oil palm trunk bagasse for succinic acid production

Nurul Adela Bukhari, Jamaliah Md Jahim, Soh Kheang Loh, Nasrin Abu Bakar, Abdullah Amru Indera Luthfi

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The exploitation of agroindustrial lignocellulose, such as oil palm trunk bagasse (OPTB), as a raw material in the production of succinic acid (SA) may serve as an effective strategy to propel the bio-based industry. This study aimed to optimise the recovery of fermentable sugar, i.e., glucose, from enzymatic hydrolysis of the dilute acid pretreated OPTB (DA-OPTB). The dilute acid pretreatment used in this study was able to remove 59.5% of hemicellulose and 13.3% of lignin. Response surface methodology (RSM) based on central composite design (CCD) was then applied to investigate four independent variables - enzyme loading (10 to 50 U/g), agitation speed (50 to 250 rpm), reaction time (0 to 96 h), and surfactant concentration (0.025 to 0.125%, v/v). The experimental glucose concentration of 21.7 g/L was in good agreement with the RSM-predicted value of 20.5 g/L. Among the parameters investigated, supplementation of a surfactant during enzymatic hydrolysis was significant in influencing glucose recovery, while the extent of the agitation speed was the least influential. The maximum recovered glucose was estimated at 217 g per kg of raw OPTB, with 7.3 g/L of SA attainable from the fermented DA-OPTB hydrolysate using Actinobacillus succinogenes 130Z. The results demonstrated that OPTB can be practically utilised in the economical production of high value-added SA.

Original languageEnglish
Pages (from-to)1679-1693
Number of pages15
JournalBioResources
Volume14
Issue number1
DOIs
Publication statusPublished - 1 Feb 2019

Fingerprint

Bagasse
Palm oil
Succinic Acid
Glucose
Acids
Enzymatic hydrolysis
oil
acid
glucose
Surface-Active Agents
Recovery
surfactant
Lignin
hydrolysis
Surface active agents
Sugars
Raw materials
lignin
bagasse
sugar

Keywords

  • Central composite design
  • Fermentable sugar
  • Lignocellulose
  • Oil palm biomass
  • Succinic acid

ASJC Scopus subject areas

  • Environmental Engineering
  • Bioengineering
  • Waste Management and Disposal

Cite this

Response surface optimisation of enzymatically hydrolysed and dilute acid pretreated oil palm trunk bagasse for succinic acid production. / Bukhari, Nurul Adela; Md Jahim, Jamaliah; Loh, Soh Kheang; Bakar, Nasrin Abu; Luthfi, Abdullah Amru Indera.

In: BioResources, Vol. 14, No. 1, 01.02.2019, p. 1679-1693.

Research output: Contribution to journalArticle

Bukhari, Nurul Adela ; Md Jahim, Jamaliah ; Loh, Soh Kheang ; Bakar, Nasrin Abu ; Luthfi, Abdullah Amru Indera. / Response surface optimisation of enzymatically hydrolysed and dilute acid pretreated oil palm trunk bagasse for succinic acid production. In: BioResources. 2019 ; Vol. 14, No. 1. pp. 1679-1693.
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