Response Surface Methodology for Biobutanol Optimization Using Locally Isolated Clostridium acetobutylicum YM1

Najeeb Kaid Nasser Al-Shorgani, Mohd Hafez Mohd Isa, Wan Mohtar Wan Yusoff, Mohd. Sahaid Kalil, Aidil Abdul Hamid

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

In this study, response surface methodology (RSM) was applied to optimize and investigate the ability of yeast extract, CaCO<inf>3</inf>, MgSO<inf>4</inf>, and K<inf>2</inf>HPO<inf>4</inf> to maximize biobutanol production by a novel local isolate of Clostridium acetobutylicum YM1. A central composite design was applied as the experimental design, and analysis of variance (ANOVA) was used to analyze the experimental data. A quadratic polynomial equation was obtained for biobutanol production by multiple regression analysis. ANOVA analysis showed that the model was significant (p < 0.0001), and the yeast extract, CaCO<inf>3</inf>, and MgSO<inf>4</inf> concentrations had a significant effect on biobutanol production. However, K<inf>2</inf>HPO<inf>4</inf> did not have a significant effect on biobutanol production. The estimated optimum combinations for biobutanol production using C. acetobutylicum YM1 were 2 g/L yeast extract, 6 g/L CaCO<inf>3</inf>, 0.1 g/L MgSO<inf>4</inf>, and 1.1 g/L K<inf>2</inf>HPO<inf>4</inf>. Subsequently, the model was validated through use of the estimated optimum conditions, which confirmed the model validity and 13.67 g/L of biobutanol was produced.

Original languageEnglish
Pages (from-to)1236-1243
Number of pages8
JournalInternational Journal of Green Energy
Volume12
Issue number12
DOIs
Publication statusPublished - 2 Dec 2015

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Clostridium
Analysis of variance (ANOVA)
Yeast
Regression analysis
Design of experiments
Polynomials
Composite materials

Keywords

  • Biobutanol
  • Clostridium acetobutylicum YM1
  • Medium optimization
  • Renewable energy
  • Response surface methodology

ASJC Scopus subject areas

  • Renewable Energy, Sustainability and the Environment

Cite this

Response Surface Methodology for Biobutanol Optimization Using Locally Isolated Clostridium acetobutylicum YM1. / Nasser Al-Shorgani, Najeeb Kaid; Mohd Isa, Mohd Hafez; Wan Yusoff, Wan Mohtar; Kalil, Mohd. Sahaid; Abdul Hamid, Aidil.

In: International Journal of Green Energy, Vol. 12, No. 12, 02.12.2015, p. 1236-1243.

Research output: Contribution to journalArticle

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