Resistance mechanism of Leptochloa chinensis Nees to propanil

Ismail Sahid, Juliana Karso, Tse Seng Chuah

Research output: Contribution to journalArticle

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Abstract

The resistance mechanism of Leptochloa chinensis Nees to propanil was investigated, based on propanil metabolism, aryl acylamidase activity, and chlorophyll fluorescence at the 8 week growth stage of L. chinensis. The concentration of propanil in the leaf and culm extracts of the resistant (R) and susceptible (S) biotypes, as measured by gas chromatography (GC), was found to increase after propanil treatment. The concentration of propanil in the leaf and culm extracts of the S biotype at 72h was 1.55 and 0.49μgmL-1, respectively. However, a lower concentration of propanil was observed in the R biotype, as compared to that in the S biotype. The residue of 3,4-dichloroaniline, as measured by GC, was detected only in the leaf extracts of the R biotype. In contrast, no residue of 3,4-dichloroaniline was observed in the S biotype. The level of aryl acylamidase in the leaf tissue extracts of the R biotype was ∼140% higher than that in the S biotype. The fluorescence studies showed that propanil inhibited the quantum efficiency of the photosystem II in both the R and S biotypes after 2h of incubation time. However, when the leaf disks were transferred and incubated in deionized water for 48h, the quantum efficiency increased in the R biotype but decreased in the S biotype. These results suggest that propanil metabolism, enhanced by aryl acylamidase activity, is the most likely factor contributing towards the mechanism of propanil resistance in L. chinensis plants at the 8 week growth stage.

Original languageEnglish
Pages (from-to)57-63
Number of pages7
JournalWeed Biology and Management
Volume11
Issue number2
DOIs
Publication statusPublished - Jun 2011

Fingerprint

Leptochloa chinensis
propanil
resistance mechanisms
biotypes
amidase
leaves
extracts
gas chromatography
developmental stages
fluorescence
metabolism
leaf extracts
photosystem II

Keywords

  • Aryl acylamidase
  • Chlorophyll fluorescence
  • Leptochloa chinensis (L.) Nees
  • Metabolism
  • Propanil

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

Resistance mechanism of Leptochloa chinensis Nees to propanil. / Sahid, Ismail; Karso, Juliana; Chuah, Tse Seng.

In: Weed Biology and Management, Vol. 11, No. 2, 06.2011, p. 57-63.

Research output: Contribution to journalArticle

Sahid, Ismail ; Karso, Juliana ; Chuah, Tse Seng. / Resistance mechanism of Leptochloa chinensis Nees to propanil. In: Weed Biology and Management. 2011 ; Vol. 11, No. 2. pp. 57-63.
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