Residual effects of TMOF-Bti formulations against 1 st instar Aedes aegypti Linnaeus larvae outside laboratory

A. N. Saiful, M. S. Lau, S. Sulaiman, Hidayatul Fathi Othman

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Objective: To evaluate the effectiveness and residual effects of trypsin modulating oostatic factor-Bacillus thuringiensis israeliensis (TMOF-Bti) formulations against Aedes aegypti (Ae. aegypti) (L.) larvae at UKM Campus Kuala Lumpur. Methods: Twenty first instar Ae. aegypti larvae were added in each bucket containing 4 L of water supplied with crushed dried leaf powder as their source of food. Combination of TMOF-Bti in rice husk formulation with the following weights viz 10, 25, 50 and 100 mg, respectively in duplicate was distributed in the buckets; while TMOF-Bti in wettable powder formulation each weighing viz 2, 5, 10 and 20 mg, respectively in duplicate was also placed in the buckets. The control buckets run in duplicate with 4 L of water and 20 first instar Ae. aegypti larvae. All buckets were covered with mosquito netting. Larval mortality was recorded after 24 hours and weekly for five weeks. A new batch of 20 1 st instar larvae Ae. aegypti was introduced into each bucket weekly without additional TMOF-Bti rice husk formulation or wettable powder. The experiment was repeated for four times. Results: The result of the study showed that all formulations were very effective on the first two weeks by giving 100%larval mortality for all concentrations applied. The TMOF (2%) + Bti (2%) had a good residual effect until the end of 3 rd week, TMOF (4%) + Bti (4%) until 4 th week, wettable powder TMOF (20%) + Bti (20%) until the third week. Conclusions: From the results it can be concluded that the TMOF-Bti formulations can be utilized in dengue vector control.

Original languageEnglish
Pages (from-to)315-319
Number of pages5
JournalAsian Pacific Journal of Tropical Biomedicine
Volume2
Issue number4
DOIs
Publication statusPublished - Apr 2012

Fingerprint

Bacillus thuringiensis
Aedes
Bacilli
Trypsin
Larva
Powders
Mosquito Nets
Dengue
Mortality
Water
Weighing
Weights and Measures
Food
Peptide oostatic hormone
Experiments

Keywords

  • 1 instar
  • Aedes aegypti
  • Dengue
  • Dengue vector control
  • Larvae
  • Larval mortality
  • Mosquito
  • Outside laboratory
  • Residual effect
  • TMOF-Bti formulations

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology (miscellaneous)

Cite this

Residual effects of TMOF-Bti formulations against 1 st instar Aedes aegypti Linnaeus larvae outside laboratory. / Saiful, A. N.; Lau, M. S.; Sulaiman, S.; Othman, Hidayatul Fathi.

In: Asian Pacific Journal of Tropical Biomedicine, Vol. 2, No. 4, 04.2012, p. 315-319.

Research output: Contribution to journalArticle

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abstract = "Objective: To evaluate the effectiveness and residual effects of trypsin modulating oostatic factor-Bacillus thuringiensis israeliensis (TMOF-Bti) formulations against Aedes aegypti (Ae. aegypti) (L.) larvae at UKM Campus Kuala Lumpur. Methods: Twenty first instar Ae. aegypti larvae were added in each bucket containing 4 L of water supplied with crushed dried leaf powder as their source of food. Combination of TMOF-Bti in rice husk formulation with the following weights viz 10, 25, 50 and 100 mg, respectively in duplicate was distributed in the buckets; while TMOF-Bti in wettable powder formulation each weighing viz 2, 5, 10 and 20 mg, respectively in duplicate was also placed in the buckets. The control buckets run in duplicate with 4 L of water and 20 first instar Ae. aegypti larvae. All buckets were covered with mosquito netting. Larval mortality was recorded after 24 hours and weekly for five weeks. A new batch of 20 1 st instar larvae Ae. aegypti was introduced into each bucket weekly without additional TMOF-Bti rice husk formulation or wettable powder. The experiment was repeated for four times. Results: The result of the study showed that all formulations were very effective on the first two weeks by giving 100{\%}larval mortality for all concentrations applied. The TMOF (2{\%}) + Bti (2{\%}) had a good residual effect until the end of 3 rd week, TMOF (4{\%}) + Bti (4{\%}) until 4 th week, wettable powder TMOF (20{\%}) + Bti (20{\%}) until the third week. Conclusions: From the results it can be concluded that the TMOF-Bti formulations can be utilized in dengue vector control.",
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AU - Othman, Hidayatul Fathi

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