Repair of segmental load-bearing bone defect by autologous mesenchymal stem cells and plasma-derived fibrin impregnated ceramic block results in early recovery of limb function

Min Hwei Ng, Suryasmi Duski, Kok Keong Tan, Mohd Reusmaazran Yusof, Kiat Cheong Low, Isa Mohamed Rose, Zahiah Mohamed, Aminuddin Bin Saim, Ruszymah Idrus

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Calcium phosphate-based bone substitutes have not been used to repair load-bearing bone defects due to their weak mechanical property. In this study, we reevaluated the functional outcomes of combining ceramic block with osteogenic-induced mesenchymal stem cells and platelet-rich plasma (TEB) to repair critical-sized segmental tibial defect. Comparisons were made with fresh marrow-impregnated ceramic block (MIC) and partially demineralized allogeneic bone block (ALLO). Six New Zealand White female rabbits were used in each study group and three rabbits with no implants were used as negative controls. By Day 90, 4/6 rabbits in TEB group and 2/6 in ALLO and MIC groups resumed normal gait pattern. Union was achieved significantly faster in TEB group with a radiological score of 4.50 ± 0.78 versus ALLO (1.06 ± 0.32), MIC (1.28 ± 0.24), and negative controls (0). Histologically, TEB group scored the highest percentage of new bone (82% ± 5.1%) compared to ALLO (5% ± 2.5%) and MIC (26% ± 5.2%). Biomechanically, TEB-treated tibiae achieved the highest compressive strength (43.50 ± 12.72 MPa) compared to those treated with ALLO (15.15 ± 3.57 MPa) and MIC (23.28 ± 6.14 MPa). In conclusion, TEB can repair critical-sized segmental load-bearing bone defects and restore limb function.

Original languageEnglish
Article number345910
JournalBioMed Research International
Volume2014
DOIs
Publication statusPublished - 2014

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Bearings (structural)
Recovery of Function
Weight-Bearing
Ceramics
Stem cells
Fibrin
Mesenchymal Stromal Cells
Bone
Repair
Extremities
Plasmas
Bone and Bones
Recovery
Bone Marrow
Defects
Rabbits
Bone Substitutes
Platelets
Compressive strength
Compressive Strength

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Repair of segmental load-bearing bone defect by autologous mesenchymal stem cells and plasma-derived fibrin impregnated ceramic block results in early recovery of limb function. / Ng, Min Hwei; Duski, Suryasmi; Tan, Kok Keong; Yusof, Mohd Reusmaazran; Low, Kiat Cheong; Mohamed Rose, Isa; Mohamed, Zahiah; Bin Saim, Aminuddin; Idrus, Ruszymah.

In: BioMed Research International, Vol. 2014, 345910, 2014.

Research output: Contribution to journalArticle

Ng, Min Hwei ; Duski, Suryasmi ; Tan, Kok Keong ; Yusof, Mohd Reusmaazran ; Low, Kiat Cheong ; Mohamed Rose, Isa ; Mohamed, Zahiah ; Bin Saim, Aminuddin ; Idrus, Ruszymah. / Repair of segmental load-bearing bone defect by autologous mesenchymal stem cells and plasma-derived fibrin impregnated ceramic block results in early recovery of limb function. In: BioMed Research International. 2014 ; Vol. 2014.
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