Reheating of soy oil is detrimental to bone metabolism in oestrogen deficient rats

Ahmad Nazrun Shuid, L. J. Yee, H. C. Loh, S. F. Yew, Norazlina Mohamed, M. T. Abdul Gapor, Kamsiah Jaarin, Ima Nirwana Soelaiman

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Introduction: The short-term and long-term effects of heated soy oil on bone metabolism in ovariectomised Sprague-Dawley rats were studied. Methods: Three-month-old female rats, were divided into five groups: normal control (NC); ovariectomised control (OVXC); ovariectomised and fed rat chow with added fresh soybean oil (SOF) or once-heated soy oil (SO1) or five-times-heated soy oil (SO5). Short-term parameters measured after one month were serum interleukin-6 (IL-6) and osteocalcin. Long-term parameters measured after six months were the structural bone histomorphometrical parameters. Vitamin E content in the soy oil subjected to the different heating treatments were also measured. Results: Rats in the SO5 group had higher levels of IL-6 after one month compared to the other four groups. Osteocalcin levels in the SO1 and SO5 groups remained high after treatment, while those in the NC and SOF groups declined. After six months, bone mass declined in the SO5 group. Vitamin E assay in the oils showed that levels of alpha-tocopherol decreased after heating the oil once and five times, while levels of gamma-and delta-tocopherols only declined after heating five times. Conclusion: Repeated heating of soy oil destroyed the tocopherols causing raised serum IL-6 and osteocalcin levels, leading to increased bone resorption and osteoporosis in the long term.

Original languageEnglish
Pages (from-to)200-206
Number of pages7
JournalSingapore Medical Journal
Volume48
Issue number3
Publication statusPublished - Mar 2007

Fingerprint

Estrogens
Oils
Bone and Bones
Heating
Osteocalcin
Interleukin-6
Vitamin E
gamma-Tocopherol
Control Groups
Soybean Oil
Tocopherols
alpha-Tocopherol
Bone Resorption
Serum
Osteoporosis
Sprague Dawley Rats

Keywords

  • Bone metabolism
  • Heated soy oil
  • Oestrogen deficiency
  • Soy oil
  • Vitamin E

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Reheating of soy oil is detrimental to bone metabolism in oestrogen deficient rats. / Shuid, Ahmad Nazrun; Yee, L. J.; Loh, H. C.; Yew, S. F.; Mohamed, Norazlina; Abdul Gapor, M. T.; Jaarin, Kamsiah; Soelaiman, Ima Nirwana.

In: Singapore Medical Journal, Vol. 48, No. 3, 03.2007, p. 200-206.

Research output: Contribution to journalArticle

Shuid, AN, Yee, LJ, Loh, HC, Yew, SF, Mohamed, N, Abdul Gapor, MT, Jaarin, K & Soelaiman, IN 2007, 'Reheating of soy oil is detrimental to bone metabolism in oestrogen deficient rats', Singapore Medical Journal, vol. 48, no. 3, pp. 200-206.
Shuid, Ahmad Nazrun ; Yee, L. J. ; Loh, H. C. ; Yew, S. F. ; Mohamed, Norazlina ; Abdul Gapor, M. T. ; Jaarin, Kamsiah ; Soelaiman, Ima Nirwana. / Reheating of soy oil is detrimental to bone metabolism in oestrogen deficient rats. In: Singapore Medical Journal. 2007 ; Vol. 48, No. 3. pp. 200-206.
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N2 - Introduction: The short-term and long-term effects of heated soy oil on bone metabolism in ovariectomised Sprague-Dawley rats were studied. Methods: Three-month-old female rats, were divided into five groups: normal control (NC); ovariectomised control (OVXC); ovariectomised and fed rat chow with added fresh soybean oil (SOF) or once-heated soy oil (SO1) or five-times-heated soy oil (SO5). Short-term parameters measured after one month were serum interleukin-6 (IL-6) and osteocalcin. Long-term parameters measured after six months were the structural bone histomorphometrical parameters. Vitamin E content in the soy oil subjected to the different heating treatments were also measured. Results: Rats in the SO5 group had higher levels of IL-6 after one month compared to the other four groups. Osteocalcin levels in the SO1 and SO5 groups remained high after treatment, while those in the NC and SOF groups declined. After six months, bone mass declined in the SO5 group. Vitamin E assay in the oils showed that levels of alpha-tocopherol decreased after heating the oil once and five times, while levels of gamma-and delta-tocopherols only declined after heating five times. Conclusion: Repeated heating of soy oil destroyed the tocopherols causing raised serum IL-6 and osteocalcin levels, leading to increased bone resorption and osteoporosis in the long term.

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