Regeneration of adrenal cortical tissue after adrenal autotransplantation

Nabishah Mohamad, B. A K Khalid, P. B. Morat, A. Zanariyah

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

This study tested the possibility of adrenal autotransplantation in rats. Since the cortex and the medulla of the adrenal gland were from different origin embriologically, either whole adrenal glands (ADR), or capsule and cortex (CAP) or medulla (MED) were autotransplanted in the subcutaneous tissue. The functions of regerated adrenal nodules were tested by measuring plasma corticosterone levels every fortnight. At the end of 9 weeks the rats were exposed to hypovolemic shock followed by naloxone injection to reverse the shock response. Results showed that rats transplanted with either cortex or whole adrenal started secreting corticosterone at 5 weeks post-transplantation (107.73 ± 21.98 ng/ml, 126.04 ± 48.41 ng/ml, respectively). Corticosterone levels increased to the value which were not significantly different from control by 9 weeks post-transplantation. However, rats transplanted with adrenal medulla showed very low corticosterone levels. Nine weeks post-transplantation, the mean blood pressure (MBP) of the CAP group was 135 ± 13 mmHg and was not significantly different from sham-operated controls, whereas MBP of MED group was significantly lower than sham-operated animals (99 ± 11 mmHg versus 141 ± 9 mmHg). The MBP of the ADR group was also lower compared to sham-operated controls (112 ± 17 mmHg P < 0.05). The MBP of the adrenal group was not statistically significant compared to the CAP group. After 1% body weight haemorrhage, the MBP decreased significantly in ADR (45 ± 5 mmHg, P < 0.05) and MED group (36 ± 9 mmHg, P < 0.001) compared to sham-operated rats (78 ± 11 mmHg) but not in the CAP (56 ± 9 mmHg). It was concluded that autotransplanted whole adrenal or adrenocortical tissues survived subcutaneously and produced sufficient corticosterone to alleviate haemorrhagic shock. Adrenal medullary tissue failed to regenerate subcutaneously and the presence of adrenal medullary tissue may suppressed the growth of transplanted adrenal gland. Nine weeks post-transplantation, the mean blood pressure (MBP) of the CAP group was 135 ± 13 mmHg and was not significantly different from sham-operated controls, whereas MBP of MED group was significantly lower than sham-operated animals (99 ± 11 mmHg versus 141 ± 9 mmHg). The MBP of the ADR group was also lower compared to sham-operated controls (112 ± 17 mmHg P < 0.05). The MBP of the adrenal group was not statistically significant compared to the CAP group. After 1% body weight haemorrhage, the MBP decreased significantly in ADR (45 ± 5 mmHg, P < 0.05) and MED group (36 ± 9 mmHg, P < 0.001) compared to sham-operated rats (78 ± 11 mmHg) but not in the CAP (56 ± 9 mmHg). It was concluded that autotransplanted whole adrenal or adrenocortical tissues survived subcutaneously and produced sufficient corticosterone to alleviate haemorrhagic shock. Adrenal medullary tissue failed to regenerate subcutaneously and the presence of adrenal medullary tissue may suppressed the growth of transplanted adrenal gland.

Original languageEnglish
Pages (from-to)419-424
Number of pages6
JournalExperimental and Clinical Endocrinology and Diabetes
Volume106
Issue number5
Publication statusPublished - 1998

Fingerprint

Autologous Transplantation
Regeneration
Adrenal Glands
Blood Pressure
Corticosterone
Transplantation
Hemorrhagic Shock
Shock
Body Weight
Hemorrhage
Adrenal Medulla
Subcutaneous Tissue
Naloxone
Growth
Capsules
Injections

Keywords

  • Adrenal gland
  • Corticosterone
  • Transplant

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Regeneration of adrenal cortical tissue after adrenal autotransplantation. / Mohamad, Nabishah; Khalid, B. A K; Morat, P. B.; Zanariyah, A.

In: Experimental and Clinical Endocrinology and Diabetes, Vol. 106, No. 5, 1998, p. 419-424.

Research output: Contribution to journalArticle

Mohamad, Nabishah ; Khalid, B. A K ; Morat, P. B. ; Zanariyah, A. / Regeneration of adrenal cortical tissue after adrenal autotransplantation. In: Experimental and Clinical Endocrinology and Diabetes. 1998 ; Vol. 106, No. 5. pp. 419-424.
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N2 - This study tested the possibility of adrenal autotransplantation in rats. Since the cortex and the medulla of the adrenal gland were from different origin embriologically, either whole adrenal glands (ADR), or capsule and cortex (CAP) or medulla (MED) were autotransplanted in the subcutaneous tissue. The functions of regerated adrenal nodules were tested by measuring plasma corticosterone levels every fortnight. At the end of 9 weeks the rats were exposed to hypovolemic shock followed by naloxone injection to reverse the shock response. Results showed that rats transplanted with either cortex or whole adrenal started secreting corticosterone at 5 weeks post-transplantation (107.73 ± 21.98 ng/ml, 126.04 ± 48.41 ng/ml, respectively). Corticosterone levels increased to the value which were not significantly different from control by 9 weeks post-transplantation. However, rats transplanted with adrenal medulla showed very low corticosterone levels. Nine weeks post-transplantation, the mean blood pressure (MBP) of the CAP group was 135 ± 13 mmHg and was not significantly different from sham-operated controls, whereas MBP of MED group was significantly lower than sham-operated animals (99 ± 11 mmHg versus 141 ± 9 mmHg). The MBP of the ADR group was also lower compared to sham-operated controls (112 ± 17 mmHg P < 0.05). The MBP of the adrenal group was not statistically significant compared to the CAP group. After 1% body weight haemorrhage, the MBP decreased significantly in ADR (45 ± 5 mmHg, P < 0.05) and MED group (36 ± 9 mmHg, P < 0.001) compared to sham-operated rats (78 ± 11 mmHg) but not in the CAP (56 ± 9 mmHg). It was concluded that autotransplanted whole adrenal or adrenocortical tissues survived subcutaneously and produced sufficient corticosterone to alleviate haemorrhagic shock. Adrenal medullary tissue failed to regenerate subcutaneously and the presence of adrenal medullary tissue may suppressed the growth of transplanted adrenal gland. Nine weeks post-transplantation, the mean blood pressure (MBP) of the CAP group was 135 ± 13 mmHg and was not significantly different from sham-operated controls, whereas MBP of MED group was significantly lower than sham-operated animals (99 ± 11 mmHg versus 141 ± 9 mmHg). The MBP of the ADR group was also lower compared to sham-operated controls (112 ± 17 mmHg P < 0.05). The MBP of the adrenal group was not statistically significant compared to the CAP group. After 1% body weight haemorrhage, the MBP decreased significantly in ADR (45 ± 5 mmHg, P < 0.05) and MED group (36 ± 9 mmHg, P < 0.001) compared to sham-operated rats (78 ± 11 mmHg) but not in the CAP (56 ± 9 mmHg). It was concluded that autotransplanted whole adrenal or adrenocortical tissues survived subcutaneously and produced sufficient corticosterone to alleviate haemorrhagic shock. Adrenal medullary tissue failed to regenerate subcutaneously and the presence of adrenal medullary tissue may suppressed the growth of transplanted adrenal gland.

AB - This study tested the possibility of adrenal autotransplantation in rats. Since the cortex and the medulla of the adrenal gland were from different origin embriologically, either whole adrenal glands (ADR), or capsule and cortex (CAP) or medulla (MED) were autotransplanted in the subcutaneous tissue. The functions of regerated adrenal nodules were tested by measuring plasma corticosterone levels every fortnight. At the end of 9 weeks the rats were exposed to hypovolemic shock followed by naloxone injection to reverse the shock response. Results showed that rats transplanted with either cortex or whole adrenal started secreting corticosterone at 5 weeks post-transplantation (107.73 ± 21.98 ng/ml, 126.04 ± 48.41 ng/ml, respectively). Corticosterone levels increased to the value which were not significantly different from control by 9 weeks post-transplantation. However, rats transplanted with adrenal medulla showed very low corticosterone levels. Nine weeks post-transplantation, the mean blood pressure (MBP) of the CAP group was 135 ± 13 mmHg and was not significantly different from sham-operated controls, whereas MBP of MED group was significantly lower than sham-operated animals (99 ± 11 mmHg versus 141 ± 9 mmHg). The MBP of the ADR group was also lower compared to sham-operated controls (112 ± 17 mmHg P < 0.05). The MBP of the adrenal group was not statistically significant compared to the CAP group. After 1% body weight haemorrhage, the MBP decreased significantly in ADR (45 ± 5 mmHg, P < 0.05) and MED group (36 ± 9 mmHg, P < 0.001) compared to sham-operated rats (78 ± 11 mmHg) but not in the CAP (56 ± 9 mmHg). It was concluded that autotransplanted whole adrenal or adrenocortical tissues survived subcutaneously and produced sufficient corticosterone to alleviate haemorrhagic shock. Adrenal medullary tissue failed to regenerate subcutaneously and the presence of adrenal medullary tissue may suppressed the growth of transplanted adrenal gland. Nine weeks post-transplantation, the mean blood pressure (MBP) of the CAP group was 135 ± 13 mmHg and was not significantly different from sham-operated controls, whereas MBP of MED group was significantly lower than sham-operated animals (99 ± 11 mmHg versus 141 ± 9 mmHg). The MBP of the ADR group was also lower compared to sham-operated controls (112 ± 17 mmHg P < 0.05). The MBP of the adrenal group was not statistically significant compared to the CAP group. After 1% body weight haemorrhage, the MBP decreased significantly in ADR (45 ± 5 mmHg, P < 0.05) and MED group (36 ± 9 mmHg, P < 0.001) compared to sham-operated rats (78 ± 11 mmHg) but not in the CAP (56 ± 9 mmHg). It was concluded that autotransplanted whole adrenal or adrenocortical tissues survived subcutaneously and produced sufficient corticosterone to alleviate haemorrhagic shock. Adrenal medullary tissue failed to regenerate subcutaneously and the presence of adrenal medullary tissue may suppressed the growth of transplanted adrenal gland.

KW - Adrenal gland

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