Reflectance based sensor for carrageenan utilizing methylene blue embedded acrylic microspheres

Yew Pei Ling, Yook Heng Lee

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Studies were conducted to understand the complexation behavior of carrageenans and methylene blue (MB) under immobilized conditions to build a novel carrageenan sensor. The sensor was an optical device based on reflectance changes due to the change of MB color (blue to purple) on exposure to carrageenan. MB was immobilized on poly(n-butylacrylate-co-N- acryloxysuccinimide) [poly(nBA-NAS)] microspheres and were coated onto a plastic support to fabricate the sensor. The sensor response was found to be related to the formation of the MB-carrageenan complexes and was more selective to Iota-carrageenan when compared with other types of carrageenans, e.g. Lambda- and Kappa-carrageenans. Besides, the sensor also showed no response to other gelling agents such as alginate and agar. Thus, the sensor exhibited a linear response range to Iota-carrageenan from 80 to 5000 ppm with a sensitivity of 377.5 ± 1.37 ΔIntensity/decade (R2 = 0.980). Lower detection limit of the sensor was 80 ppm, whilst repeatability and reproducibility were 0.36% and 0.31% relative standard deviation, respectively. Moreover, the sensor was useful in analyzing carrageenan in real samples with 83-117% recovery values.

Original languageEnglish
Pages (from-to)247-252
Number of pages6
JournalSensors and Actuators, B: Chemical
Volume192
DOIs
Publication statusPublished - 2014

Fingerprint

Carrageenan
Methylene Blue
methylene blue
Microspheres
Acrylics
reflectance
sensors
Sensors
Alginate
Optical devices
Complexation
standard deviation
plastics
recovery
Agar
Plastics
Color
color
Recovery
sensitivity

Keywords

  • Carrageenan
  • Gelling agents
  • Methylene blue
  • N-acryloxysuccinimide
  • n-Butyl acrylate
  • Reflectance measurement

ASJC Scopus subject areas

  • Instrumentation
  • Materials Chemistry
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Electrical and Electronic Engineering

Cite this

Reflectance based sensor for carrageenan utilizing methylene blue embedded acrylic microspheres. / Ling, Yew Pei; Lee, Yook Heng.

In: Sensors and Actuators, B: Chemical, Vol. 192, 2014, p. 247-252.

Research output: Contribution to journalArticle

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