Reduction of DNA damage in older healthy adults by Tri E® Tocotrienol supplementation

Siok Fong Chin, Noor Aini Abdul Hamid, Azian Abdul Latiff, Zaiton Zakaria, Musalmah Mazlan, Yasmin Anum Mohd Yusof, Aminuddin Abdul Karim, Johari Ibahim, Zalina Hamid, Wan Zurinah Wan Ngah

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Objective: The free radical theory of aging (FRTA) suggests that free radicals are the leading cause of deteriorating physiologic function during senescence. Free radicals attack cellular structures or molecules such as DNA resulting in various modifications to the DNA structures. Accumulation of unrepaired DNA contributes to a variety of disorders associated with the aging process. Methods: A randomized, double-blinded placebo-controlled study was undertaken to evaluate the effect of Tri E® Tocotrienol on DNA damage. Sixty four subjects 37-78 y old completed the study. A daily dose of 160 mg of Tri E® Tocotrienol was given for 6 months. Blood samples were analyzed for DNA damage using comet assay, frequency of sister chromatid exchange (SCE), and chromosome 4 aberrations. Results: Results showed a significant reduction in DNA damage as measured by comet assay after 3 mo (P < 0.01) and remained low at 6 mo (P < 0.01). The frequency of SCE was also reduced after 6 mo of supplementation (P < 0.05), albeit more markedly in the >50 y-old group (P < 0.01) whereas urinary 8-hydroxy-2′-deoxyguanosine (8-OHdG) levels were significantly reduced (P < 0.05). A strong positive correlation was observed between SCE with age, whereas weak positive correlations were observed in DNA damage and 8-OHdG, which were reduced with supplementation. However, no translocation or a stable insertion was observed in chromosome 4. Conclusion: Tri E® Tocotrienol supplementation may be beneficial by reducing DNA damage as indicated by a reduction in DNA damage, SCE frequency, and urinary 8-OHdG.

Original languageEnglish
Pages (from-to)1-10
Number of pages10
JournalNutrition
Volume24
Issue number1
DOIs
Publication statusPublished - Jan 2008

Fingerprint

Tocotrienols
DNA Damage
Sister Chromatid Exchange
Free Radicals
Chromosomes, Human, Pair 4
Comet Assay
DNA
Cellular Structures
Chromosome Aberrations
Placebos

Keywords

  • 8-Hydroxy-2′-deoxyguanosine
  • Chromosome 4
  • DNA damage
  • Sister chromatid exchange

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Surgery
  • Medicine (miscellaneous)

Cite this

Chin, S. F., Hamid, N. A. A., Latiff, A. A., Zakaria, Z., Mazlan, M., Mohd Yusof, Y. A., ... Ngah, W. Z. W. (2008). Reduction of DNA damage in older healthy adults by Tri E® Tocotrienol supplementation. Nutrition, 24(1), 1-10. https://doi.org/10.1016/j.nut.2007.08.006

Reduction of DNA damage in older healthy adults by Tri E® Tocotrienol supplementation. / Chin, Siok Fong; Hamid, Noor Aini Abdul; Latiff, Azian Abdul; Zakaria, Zaiton; Mazlan, Musalmah; Mohd Yusof, Yasmin Anum; Karim, Aminuddin Abdul; Ibahim, Johari; Hamid, Zalina; Ngah, Wan Zurinah Wan.

In: Nutrition, Vol. 24, No. 1, 01.2008, p. 1-10.

Research output: Contribution to journalArticle

Chin, SF, Hamid, NAA, Latiff, AA, Zakaria, Z, Mazlan, M, Mohd Yusof, YA, Karim, AA, Ibahim, J, Hamid, Z & Ngah, WZW 2008, 'Reduction of DNA damage in older healthy adults by Tri E® Tocotrienol supplementation', Nutrition, vol. 24, no. 1, pp. 1-10. https://doi.org/10.1016/j.nut.2007.08.006
Chin, Siok Fong ; Hamid, Noor Aini Abdul ; Latiff, Azian Abdul ; Zakaria, Zaiton ; Mazlan, Musalmah ; Mohd Yusof, Yasmin Anum ; Karim, Aminuddin Abdul ; Ibahim, Johari ; Hamid, Zalina ; Ngah, Wan Zurinah Wan. / Reduction of DNA damage in older healthy adults by Tri E® Tocotrienol supplementation. In: Nutrition. 2008 ; Vol. 24, No. 1. pp. 1-10.
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abstract = "Objective: The free radical theory of aging (FRTA) suggests that free radicals are the leading cause of deteriorating physiologic function during senescence. Free radicals attack cellular structures or molecules such as DNA resulting in various modifications to the DNA structures. Accumulation of unrepaired DNA contributes to a variety of disorders associated with the aging process. Methods: A randomized, double-blinded placebo-controlled study was undertaken to evaluate the effect of Tri E{\circledR} Tocotrienol on DNA damage. Sixty four subjects 37-78 y old completed the study. A daily dose of 160 mg of Tri E{\circledR} Tocotrienol was given for 6 months. Blood samples were analyzed for DNA damage using comet assay, frequency of sister chromatid exchange (SCE), and chromosome 4 aberrations. Results: Results showed a significant reduction in DNA damage as measured by comet assay after 3 mo (P < 0.01) and remained low at 6 mo (P < 0.01). The frequency of SCE was also reduced after 6 mo of supplementation (P < 0.05), albeit more markedly in the >50 y-old group (P < 0.01) whereas urinary 8-hydroxy-2′-deoxyguanosine (8-OHdG) levels were significantly reduced (P < 0.05). A strong positive correlation was observed between SCE with age, whereas weak positive correlations were observed in DNA damage and 8-OHdG, which were reduced with supplementation. However, no translocation or a stable insertion was observed in chromosome 4. Conclusion: Tri E{\circledR} Tocotrienol supplementation may be beneficial by reducing DNA damage as indicated by a reduction in DNA damage, SCE frequency, and urinary 8-OHdG.",
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AU - Mohd Yusof, Yasmin Anum

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N2 - Objective: The free radical theory of aging (FRTA) suggests that free radicals are the leading cause of deteriorating physiologic function during senescence. Free radicals attack cellular structures or molecules such as DNA resulting in various modifications to the DNA structures. Accumulation of unrepaired DNA contributes to a variety of disorders associated with the aging process. Methods: A randomized, double-blinded placebo-controlled study was undertaken to evaluate the effect of Tri E® Tocotrienol on DNA damage. Sixty four subjects 37-78 y old completed the study. A daily dose of 160 mg of Tri E® Tocotrienol was given for 6 months. Blood samples were analyzed for DNA damage using comet assay, frequency of sister chromatid exchange (SCE), and chromosome 4 aberrations. Results: Results showed a significant reduction in DNA damage as measured by comet assay after 3 mo (P < 0.01) and remained low at 6 mo (P < 0.01). The frequency of SCE was also reduced after 6 mo of supplementation (P < 0.05), albeit more markedly in the >50 y-old group (P < 0.01) whereas urinary 8-hydroxy-2′-deoxyguanosine (8-OHdG) levels were significantly reduced (P < 0.05). A strong positive correlation was observed between SCE with age, whereas weak positive correlations were observed in DNA damage and 8-OHdG, which were reduced with supplementation. However, no translocation or a stable insertion was observed in chromosome 4. Conclusion: Tri E® Tocotrienol supplementation may be beneficial by reducing DNA damage as indicated by a reduction in DNA damage, SCE frequency, and urinary 8-OHdG.

AB - Objective: The free radical theory of aging (FRTA) suggests that free radicals are the leading cause of deteriorating physiologic function during senescence. Free radicals attack cellular structures or molecules such as DNA resulting in various modifications to the DNA structures. Accumulation of unrepaired DNA contributes to a variety of disorders associated with the aging process. Methods: A randomized, double-blinded placebo-controlled study was undertaken to evaluate the effect of Tri E® Tocotrienol on DNA damage. Sixty four subjects 37-78 y old completed the study. A daily dose of 160 mg of Tri E® Tocotrienol was given for 6 months. Blood samples were analyzed for DNA damage using comet assay, frequency of sister chromatid exchange (SCE), and chromosome 4 aberrations. Results: Results showed a significant reduction in DNA damage as measured by comet assay after 3 mo (P < 0.01) and remained low at 6 mo (P < 0.01). The frequency of SCE was also reduced after 6 mo of supplementation (P < 0.05), albeit more markedly in the >50 y-old group (P < 0.01) whereas urinary 8-hydroxy-2′-deoxyguanosine (8-OHdG) levels were significantly reduced (P < 0.05). A strong positive correlation was observed between SCE with age, whereas weak positive correlations were observed in DNA damage and 8-OHdG, which were reduced with supplementation. However, no translocation or a stable insertion was observed in chromosome 4. Conclusion: Tri E® Tocotrienol supplementation may be beneficial by reducing DNA damage as indicated by a reduction in DNA damage, SCE frequency, and urinary 8-OHdG.

KW - 8-Hydroxy-2′-deoxyguanosine

KW - Chromosome 4

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KW - Sister chromatid exchange

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