Reduced DNA topoisomerase II activity in ataxia-telangiectasia cells

S. Pal Singh, R. Mohamed, C. Salmond, M. F. Lavin

    Research output: Contribution to journalArticle

    21 Citations (Scopus)

    Abstract

    Considerable evidence supports a defect at the level of chromatin structure or recognition of that structure in cells from patients with the human genetic disorder ataxia-telangiectasia. Accordingly, we have investigated the activities of enzymes that alter the topology of DNA in Epstein Barr Virus-transformed lymphoblastoid cells from patients with this syndrome. Reduced activity of DNA topoisomerase II, determined by unknotting of P4 phage DNA, was observed in partially purified extracts from 5 ataxia-telangiectasia cell lines. The level of enzyme activity was reduced substantially in 4 of these cell lines and to a lesser extent in the other cell line compared to controls. DNA topoisomerase I, assayed by relaxation of supercoiled DNA, was found to be present at comparable levels in both cell types. Reduced activity of topoisomerase II in ataxia-telangiectasia is compatible with the molecular, cellular and clinical changes described in this syndrome.

    Original languageEnglish
    Pages (from-to)3919-3929
    Number of pages11
    JournalNucleic Acids Research
    Volume16
    Issue number9
    DOIs
    Publication statusPublished - 11 May 1988

    Fingerprint

    Ataxia Telangiectasia
    Type II DNA Topoisomerase
    DNA
    Cells
    Cell Line
    Cell
    Superhelical DNA
    Type I DNA Topoisomerase
    Inborn Genetic Diseases
    Bacteriophages
    Medical Genetics
    Enzyme activity
    Enzymes
    Human Herpesvirus 4
    Viruses
    Chromatin
    Line
    Topology
    Defects
    Virus

    ASJC Scopus subject areas

    • Statistics, Probability and Uncertainty
    • Applied Mathematics
    • Health, Toxicology and Mutagenesis
    • Toxicology
    • Genetics(clinical)
    • Genetics

    Cite this

    Singh, S. P., Mohamed, R., Salmond, C., & Lavin, M. F. (1988). Reduced DNA topoisomerase II activity in ataxia-telangiectasia cells. Nucleic Acids Research, 16(9), 3919-3929. https://doi.org/10.1093/nar/16.9.3919

    Reduced DNA topoisomerase II activity in ataxia-telangiectasia cells. / Singh, S. Pal; Mohamed, R.; Salmond, C.; Lavin, M. F.

    In: Nucleic Acids Research, Vol. 16, No. 9, 11.05.1988, p. 3919-3929.

    Research output: Contribution to journalArticle

    Singh, SP, Mohamed, R, Salmond, C & Lavin, MF 1988, 'Reduced DNA topoisomerase II activity in ataxia-telangiectasia cells', Nucleic Acids Research, vol. 16, no. 9, pp. 3919-3929. https://doi.org/10.1093/nar/16.9.3919
    Singh, S. Pal ; Mohamed, R. ; Salmond, C. ; Lavin, M. F. / Reduced DNA topoisomerase II activity in ataxia-telangiectasia cells. In: Nucleic Acids Research. 1988 ; Vol. 16, No. 9. pp. 3919-3929.
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