Reconstruction of living bilayer human skin equivalent utilizing human fibrin as a scaffold

Mazlyzam Abdul Latif, B. S. Aminuddin, N. H. Fuzina, M. M. Norhayati, O. Fauziah, M. R. Isa, L. Saim, Ruszymah Idrus

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

Our aim of this study was to develop a new methodology for constructing a bilayer human skin equivalent to create a more clinical compliance skin graft composite for the treatment of various skin defects. We utilized human plasma derived fibrin as the scaffold for the development of a living bilayer human skin equivalent: fibrin-fibroblast and fibrin-keratinocyte (B-FF/FK SE). Skin cells from six consented patients were culture-expanded to passage 1. For B-FF/FK SE formation, human fibroblasts were embedded in human fibrin matrix and subsequently another layer of human keratinocytes in human fibrin matrix was stacked on top. The B-FF/FK SE was then transplanted to athymic mice model for 4 weeks to evaluate its regeneration and clinical performance. The in vivo B-FF/FK SE has similar properties as native human skin by histological analysis and expression of basal Keratin 14 gene in the epidermal layer and Collagen type I gene in the dermal layer. Electron microscopy analysis of in vivo B-FF/FK SE showed well-formed and continuous epidermal-dermal junction. We have successfully developed a technique to engineer living bilayer human skin equivalent using human fibrin matrix. The utilization of culture-expanded human skin cells and fibrin matrix from human blood will allow a fully autologous human skin equivalent construction.

Original languageEnglish
Pages (from-to)355-363
Number of pages9
JournalBurns
Volume33
Issue number3
DOIs
Publication statusPublished - May 2007

Fingerprint

Fibrin
Skin
Keratinocytes
Fibroblasts
Keratin-14
Collagen Type I
Population Groups
Nude Mice
Genes
Compliance
Regeneration
Electron Microscopy
Transplants

Keywords

  • Autologous skin equivalent
  • Fibrin
  • Fibroblasts
  • Keratinocytes
  • Skin
  • Tissue engineered skin

ASJC Scopus subject areas

  • Emergency Medicine
  • Surgery

Cite this

Reconstruction of living bilayer human skin equivalent utilizing human fibrin as a scaffold. / Abdul Latif, Mazlyzam; Aminuddin, B. S.; Fuzina, N. H.; Norhayati, M. M.; Fauziah, O.; Isa, M. R.; Saim, L.; Idrus, Ruszymah.

In: Burns, Vol. 33, No. 3, 05.2007, p. 355-363.

Research output: Contribution to journalArticle

Abdul Latif, M, Aminuddin, BS, Fuzina, NH, Norhayati, MM, Fauziah, O, Isa, MR, Saim, L & Idrus, R 2007, 'Reconstruction of living bilayer human skin equivalent utilizing human fibrin as a scaffold', Burns, vol. 33, no. 3, pp. 355-363. https://doi.org/10.1016/j.burns.2006.08.022
Abdul Latif, Mazlyzam ; Aminuddin, B. S. ; Fuzina, N. H. ; Norhayati, M. M. ; Fauziah, O. ; Isa, M. R. ; Saim, L. ; Idrus, Ruszymah. / Reconstruction of living bilayer human skin equivalent utilizing human fibrin as a scaffold. In: Burns. 2007 ; Vol. 33, No. 3. pp. 355-363.
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