Reconstruction of Curcuma aeruginosa secondary metabolite biosynthetic pathway using Omics data

Nurul Syafika Mohamad-Fauzi, Rabiatul Adawiah Zainal-Abidin, Zeti Azura Mohamed Hussein, Mohd Waznul Adly Zaidan, Sanimah Simoh, Alizah Zainal

Research output: Contribution to journalArticle

Abstract

Curcuma aeruginosa or temu hitam is herbaceous plant with high therapeutic values in its rhizome that is widely used in traditional medicine. However, molecular studies on the secondary metabolite biosynthetic pathway of C. aeruginosa is still limited. Hence, the aim of this study was to explore and reconstruct the secondary metabolite biosynthetic pathway of C. aeruginosa rhizome by integrating the metabolite profiling and transcriptomic data. A total of 81 metabolites were identified in the rhizome of C. aeruginosa; amongst others are curzerene and β-Cubebene which are potent antioxidants. A total of 28,225 unigene were obtained from the transcriptomic sequencing of C. aeruginosa rhizome and analysed to identify potential genes associated with the biosynthesis of its metabolites. Of these, 43 unigenes were identified and mapped onto five sub-pathways; i.e. carotenoid biosynthetic pathway, diterpenoid biosynthetic pathway, monoterpenoid biosynthetic pathway, terpenoid and steroid biosynthetic pathway, and sesquiterpenoid and triterpenoid biosynthetic pathway. This study demonstrated a systematic bioinformatic approach to reconstruct a metabolic pathway in the rhizome of C. aeruginosa using bioinformatic approach.

Original languageEnglish
Pages (from-to)2985-2992
Number of pages8
JournalSains Malaysiana
Volume47
Issue number12
DOIs
Publication statusPublished - 1 Dec 2018

Fingerprint

Curcuma
Biosynthetic Pathways
Rhizome
Computational Biology
Monoterpenes
Diterpenes
Terpenes
Traditional Medicine
Carotenoids
Metabolic Networks and Pathways
Antioxidants
Steroids
Genes

Keywords

  • Data integration
  • Metabolic pathway
  • Metabolomics
  • Pathway reconstruction
  • Transcriptomic

ASJC Scopus subject areas

  • General

Cite this

Reconstruction of Curcuma aeruginosa secondary metabolite biosynthetic pathway using Omics data. / Mohamad-Fauzi, Nurul Syafika; Zainal-Abidin, Rabiatul Adawiah; Mohamed Hussein, Zeti Azura; Zaidan, Mohd Waznul Adly; Simoh, Sanimah; Zainal, Alizah.

In: Sains Malaysiana, Vol. 47, No. 12, 01.12.2018, p. 2985-2992.

Research output: Contribution to journalArticle

Mohamad-Fauzi, NS, Zainal-Abidin, RA, Mohamed Hussein, ZA, Zaidan, MWA, Simoh, S & Zainal, A 2018, 'Reconstruction of Curcuma aeruginosa secondary metabolite biosynthetic pathway using Omics data', Sains Malaysiana, vol. 47, no. 12, pp. 2985-2992. https://doi.org/10.17576/jsm-2018-4712-07
Mohamad-Fauzi, Nurul Syafika ; Zainal-Abidin, Rabiatul Adawiah ; Mohamed Hussein, Zeti Azura ; Zaidan, Mohd Waznul Adly ; Simoh, Sanimah ; Zainal, Alizah. / Reconstruction of Curcuma aeruginosa secondary metabolite biosynthetic pathway using Omics data. In: Sains Malaysiana. 2018 ; Vol. 47, No. 12. pp. 2985-2992.
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