Real-time quantifi cation for BCR-ABL transcripts in chronic myeloid leukaemia patients in UKMMC, Malaysia

F. L. Wong, Noor Hamidah Hussin, A. A. Hawa, A. N. Nurul, Leong Chooi Fun, S Fadilah S. Abdul Wahid, O. Ainoon

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Molecular pathogenesis of chronic myeloid leukemia (CML) is well established and molecular monitoring for patients with CML has become an important practice in the management of patients on imatinib therapy. In the present study, we report the use of RQ-PCR method for detection of BCR-ABL fusion gene for our CML cases. We performed a two-step RQ-PCR on bone marrow aspirates or peripheral blood of 37 CML patients. Quantitative expression of BCR-ABL fusion gene was carried out relative to the expression of a housekeeping gene as endogenous control to compensate for uneven cell numbers, RNA quality, or variations in reverse transcription effi ciencies. Twenty-four of these patients were pre-treated with hydroxyurea or alpha interferon prior to the imatinib therapy. Their BCR-ABL fusion gene levels were monitored for 18 months. All samples processed were evaluable. The PCR amplifi cation effi ciency of the ABL gene is 90.5% (0.2158) and the BCR-ABL gene, 93.4% (0.1573).

Original languageEnglish
Pages (from-to)107-112
Number of pages6
JournalMalaysian Journal of Pathology
Volume33
Issue number2
Publication statusPublished - Dec 2011

Fingerprint

Malaysia
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
Cations
Gene Fusion
Polymerase Chain Reaction
Hydroxyurea
Practice Management
Essential Genes
Physiologic Monitoring
Interferon-alpha
Genes
Reverse Transcription
Cell Count
Bone Marrow
RNA
Therapeutics
Imatinib Mesylate

Keywords

  • BCR-ABL
  • CML
  • RQ-PCR

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Cell Biology
  • Histology

Cite this

Real-time quantifi cation for BCR-ABL transcripts in chronic myeloid leukaemia patients in UKMMC, Malaysia. / Wong, F. L.; Hussin, Noor Hamidah; Hawa, A. A.; Nurul, A. N.; Chooi Fun, Leong; S. Abdul Wahid, S Fadilah; Ainoon, O.

In: Malaysian Journal of Pathology, Vol. 33, No. 2, 12.2011, p. 107-112.

Research output: Contribution to journalArticle

Wong, F. L. ; Hussin, Noor Hamidah ; Hawa, A. A. ; Nurul, A. N. ; Chooi Fun, Leong ; S. Abdul Wahid, S Fadilah ; Ainoon, O. / Real-time quantifi cation for BCR-ABL transcripts in chronic myeloid leukaemia patients in UKMMC, Malaysia. In: Malaysian Journal of Pathology. 2011 ; Vol. 33, No. 2. pp. 107-112.
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