Reaction optimization of Aspergillus niger α-L-Arabinofuranosidase for improved arabinose production from kenaf stem

Siti Norbaidurah Ayob, Abdul Munir Abd. Murad, Farah Diba Abu Bakar, Rosli Md Illias

Research output: Contribution to journalArticle

Abstract

There are abundant of lignocellulosic biomass readily available with varying compositions. Kenaf (Hibiscus cannabinus) is one of this lignocellulosic biomass that has a high content of hemicellulose. This particular hemicellulose is composed of high arabinoxylan, which is a xylan backbone with arabinofuranosyl branches. In order to hydrolyze arabinoxylan, a branching enzyme is needed. Therefore, α-L-arabinofuranosidase from Aspergillus niger ATCC120120 (AnabfA) was used to hydrolyzed pre-treated kenaf and the reaction conditions were optimized using central composite design (CCD) to produce a significant amount of arabinose. There were 20 experiments conducted with 1.68 star points and 6 replicates at the centre points. The reaction conditions that were optimized are enzyme loading, substrate concentration and reaction time in which resulted with 88 U AnabfA activity, 0.9% (w/v) and 48 h, respectively. These optimized conditions managed to increase the yield of arabinose with 47.17 mg/g arabinose produced.

Original languageEnglish
Pages (from-to)137-145
Number of pages9
JournalJurnal Teknologi
Volume79
Issue number4
DOIs
Publication statusPublished - 1 May 2017

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Aspergillus
Biomass
Enzymes
Stars
Composite materials
Substrates
Chemical analysis
Experiments
Xylans

Keywords

  • Arabinofuranosidase
  • Arabinose
  • Arabinoxylan
  • Glycosyl hydrolase
  • Kenaf
  • Statictical analysis

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Reaction optimization of Aspergillus niger α-L-Arabinofuranosidase for improved arabinose production from kenaf stem. / Ayob, Siti Norbaidurah; Abd. Murad, Abdul Munir; Abu Bakar, Farah Diba; Md Illias, Rosli.

In: Jurnal Teknologi, Vol. 79, No. 4, 01.05.2017, p. 137-145.

Research output: Contribution to journalArticle

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