Rapid determination of kappa-carrageenan using a biosensor from immobilized Pseudomonas carrageenovora cells

Riyadh Abdulmalek Hassan, Yook Heng Lee, Asmat Ahmad, Ling Ling Tan @ Chong

Research output: Contribution to journalArticle

Abstract

A potentiometric whole cell biosensor based on immobilized marine bacterium, Pseudomonas carrageenovora producing κ-carrageenase and glycosulfatase enzymes for specific and direct determination of κ-carrageenan, is described. The bacterial cells were immobilized on the self-plasticized hydrogen ion (H + )-selective acrylic membrane electrode surface to form a catalytic layer. Hydrogen ionophore I was incorporated in the poly(n-butyl acrylate) [poly(nBA)] as a pH ionophore. Catalytic decomposition of κ-carrageenan by the bienzymatic cascade reaction produced neoagarobiose, an inorganic sulfate ion and a proton. The latter was detectable by H + ion transducer for indirect potentiometric quantification of κ-carrageenan concentration. The use of a disposable screen-printed Ag/AgCl electrode (SPE) provided no cleaning requirement and enabled κ-carrageenan detection to be carried out conveniently without cross contamination in a complex food sample. The SPE-based microbial biosensor response was found to be reproducible with high reproducibility and relative standard deviation (RSD) at 2.6% (n = 3). The whole cell biosensor demonstrated a broad dynamic linear response range to κ-carrageenan from 0.2–100 ppm in 20 mM phosphate buffer saline (PBS) at pH 7.5 with a detection limit at 0.05 ppm and a Nernstian sensitivity of 58.78±0.87 mV/decade (R 2 = 0.995). The biosensor showed excellent selectivity towards κ-carrageenan compared to other types of carrageenans tested e.g. ι-carrageenan and λ-car-rageenan. No pretreatment to the food sample was necessary when the developed whole cell biosensor was employed for direct assay of κ-carrageenan in dairy product.

Original languageEnglish
Article numbere0214580
JournalPLoS ONE
Volume14
Issue number4
DOIs
Publication statusPublished - 1 Apr 2019

Fingerprint

kappa carrageenan
biosensors
Carrageenan
carrageenan
Biosensing Techniques
Pseudomonas
Biosensors
cells
glycosulphatase
Ionophores
ionophores
electrodes
Protons
Electrodes
foods
Dairy products
Ions
ions
hydrogen ions
Food

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Rapid determination of kappa-carrageenan using a biosensor from immobilized Pseudomonas carrageenovora cells. / Hassan, Riyadh Abdulmalek; Lee, Yook Heng; Ahmad, Asmat; Tan @ Chong, Ling Ling.

In: PLoS ONE, Vol. 14, No. 4, e0214580, 01.04.2019.

Research output: Contribution to journalArticle

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