Rapid Detection of Sepsis using CESDA

the Caenorhabditis elegans Sepsis Detection Assay

Research output: Contribution to journalArticle

Abstract

INTRODUCTION: The nematode Caenorhabditis elegans was used as a biological sensor to detect the urine of sepsis patients (CESDA assay). METHODS: C. elegans was aliquoted onto the center of assay plates and allowed to migrate towards sepsis (T) or control (C) urine samples spotted on the same plate. The number of worms found in either (T) or (C) was scored at 10-minute intervals over a 60-minute period. RESULTS: The worms were able to identify the urine (<48 hours) of sepsis patients rapidly within 20 minutes (AUROC=0.67, p=0.012) and infection within 40 minutes (AUROC=0.80, p=0.016). CONCLUSIONS: CESDA could be further explored for sepsis diagnosis.

Original languageEnglish
Pages (from-to)e20180300
JournalRevista da Sociedade Brasileira de Medicina Tropical
Volume52
DOIs
Publication statusPublished - 14 Mar 2019

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Caenorhabditis elegans
Sepsis
Urine
Infection

ASJC Scopus subject areas

  • Parasitology
  • Microbiology (medical)
  • Infectious Diseases

Cite this

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title = "Rapid Detection of Sepsis using CESDA: the Caenorhabditis elegans Sepsis Detection Assay",
abstract = "INTRODUCTION: The nematode Caenorhabditis elegans was used as a biological sensor to detect the urine of sepsis patients (CESDA assay). METHODS: C. elegans was aliquoted onto the center of assay plates and allowed to migrate towards sepsis (T) or control (C) urine samples spotted on the same plate. The number of worms found in either (T) or (C) was scored at 10-minute intervals over a 60-minute period. RESULTS: The worms were able to identify the urine (<48 hours) of sepsis patients rapidly within 20 minutes (AUROC=0.67, p=0.012) and infection within 40 minutes (AUROC=0.80, p=0.016). CONCLUSIONS: CESDA could be further explored for sepsis diagnosis.",
author = "Tee, {Ling Fei} and Tan, {Toh Leong} and Neoh, {Hui Min} and {A. Jamal}, {A. Rahman}",
year = "2019",
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doi = "10.1590/0037-8682-0300-2018",
language = "English",
volume = "52",
pages = "e20180300",
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T1 - Rapid Detection of Sepsis using CESDA

T2 - the Caenorhabditis elegans Sepsis Detection Assay

AU - Tee, Ling Fei

AU - Tan, Toh Leong

AU - Neoh, Hui Min

AU - A. Jamal, A. Rahman

PY - 2019/3/14

Y1 - 2019/3/14

N2 - INTRODUCTION: The nematode Caenorhabditis elegans was used as a biological sensor to detect the urine of sepsis patients (CESDA assay). METHODS: C. elegans was aliquoted onto the center of assay plates and allowed to migrate towards sepsis (T) or control (C) urine samples spotted on the same plate. The number of worms found in either (T) or (C) was scored at 10-minute intervals over a 60-minute period. RESULTS: The worms were able to identify the urine (<48 hours) of sepsis patients rapidly within 20 minutes (AUROC=0.67, p=0.012) and infection within 40 minutes (AUROC=0.80, p=0.016). CONCLUSIONS: CESDA could be further explored for sepsis diagnosis.

AB - INTRODUCTION: The nematode Caenorhabditis elegans was used as a biological sensor to detect the urine of sepsis patients (CESDA assay). METHODS: C. elegans was aliquoted onto the center of assay plates and allowed to migrate towards sepsis (T) or control (C) urine samples spotted on the same plate. The number of worms found in either (T) or (C) was scored at 10-minute intervals over a 60-minute period. RESULTS: The worms were able to identify the urine (<48 hours) of sepsis patients rapidly within 20 minutes (AUROC=0.67, p=0.012) and infection within 40 minutes (AUROC=0.80, p=0.016). CONCLUSIONS: CESDA could be further explored for sepsis diagnosis.

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