Radical scavenging activity of triterpene steroids from stem of Polygonum pulchrum Bl

Sahidin, Nohong, Asrul Sani, Marianti Anggrenimanggau, Asep Sukohar, Harto Widodo, Syarul Nataqain Baharum

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Results: Four triterpene steroids; namely, (1) 6β-hydroxystigmasta-4,22-dien-3-one, (2) stigmasterol,(3) stigmasta-4,22-dien-3-one, and (4) ergosterol peroxide, were isolated and identified from stems of P. pulchrum Bl. The antioxidant activities of all compounds were indicated by IC50 value of the compounds. The values of IC50(μM) of6β-hydroxystigmasta-4,22-dien-3-one, stigmasterol, Stigmasta-4,22-dien-3-on,ergosterol peroxide, and vitamine C (standard) toward DPPH were obtained at233.4 ± 0.28; 372.3 ± 0.33; 144.80 ± 0.24; 1083.1 ± 0.38; and 68.9 ± 0.12, respectively.

Conclusions: We found that Stigmasta-4,22-dien-3-onewas the most active compound toward DPPH.

Objective: P. pulchrum grows abundantly in Kendari (Sulawesi Tenggara province, Indonesia).However, there is no report neither chemical contents nor biological activitiesof the plant. This project studiesthe isolation, structure elucidations, and radical scavenging activity evaluation of triterpene steroids from stems of P. pulchrum.

Methods: The isolation of the compounds was carried out by using chromatography method, i.e., vacuum liquid chromatography (VLC) and radial chromatography (RC) with silica gel as an adsorbent and various solvents as eluent. The compound structures were evaluated by spectroscopic data (FTIR and NMR data) and then the results were compared with the existing data from references. The antioxidant activity of these compounds was evaluated towards DPPH (1,1-diphenyl 2-picryl-hydrazyl).

Original languageEnglish
Pages (from-to)350-354
Number of pages5
JournalInternational Journal of Pharmacy and Pharmaceutical Sciences
Volume6
Issue number8
Publication statusPublished - 2014

Fingerprint

Polygonum
Triterpenes
Stigmasterol
Indonesia
Steroids
Inhibitory Concentration 50
Chromatography
Antioxidants
Silica Gel
Fourier Transform Infrared Spectroscopy
Vacuum
Liquid Chromatography
diphenyl
ergosterol-5,8-peroxide

Keywords

  • 22-dien-3-one
  • 22-dien-3-one
  • 6β-hydroxystigmasta-4
  • Ergosterol peroxide and DPPH
  • P. pulchrum Bl
  • Stigmasta-4
  • Stigmasterol

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Pharmacology

Cite this

Radical scavenging activity of triterpene steroids from stem of Polygonum pulchrum Bl. / Sahidin; Nohong; Sani, Asrul; Anggrenimanggau, Marianti; Sukohar, Asep; Widodo, Harto; Baharum, Syarul Nataqain.

In: International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 6, No. 8, 2014, p. 350-354.

Research output: Contribution to journalArticle

Sahidin, Nohong, Sani, A, Anggrenimanggau, M, Sukohar, A, Widodo, H & Baharum, SN 2014, 'Radical scavenging activity of triterpene steroids from stem of Polygonum pulchrum Bl', International Journal of Pharmacy and Pharmaceutical Sciences, vol. 6, no. 8, pp. 350-354.
Sahidin ; Nohong ; Sani, Asrul ; Anggrenimanggau, Marianti ; Sukohar, Asep ; Widodo, Harto ; Baharum, Syarul Nataqain. / Radical scavenging activity of triterpene steroids from stem of Polygonum pulchrum Bl. In: International Journal of Pharmacy and Pharmaceutical Sciences. 2014 ; Vol. 6, No. 8. pp. 350-354.
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T1 - Radical scavenging activity of triterpene steroids from stem of Polygonum pulchrum Bl

AU - Sahidin,

AU - Nohong,

AU - Sani, Asrul

AU - Anggrenimanggau, Marianti

AU - Sukohar, Asep

AU - Widodo, Harto

AU - Baharum, Syarul Nataqain

PY - 2014

Y1 - 2014

N2 - Results: Four triterpene steroids; namely, (1) 6β-hydroxystigmasta-4,22-dien-3-one, (2) stigmasterol,(3) stigmasta-4,22-dien-3-one, and (4) ergosterol peroxide, were isolated and identified from stems of P. pulchrum Bl. The antioxidant activities of all compounds were indicated by IC50 value of the compounds. The values of IC50(μM) of6β-hydroxystigmasta-4,22-dien-3-one, stigmasterol, Stigmasta-4,22-dien-3-on,ergosterol peroxide, and vitamine C (standard) toward DPPH were obtained at233.4 ± 0.28; 372.3 ± 0.33; 144.80 ± 0.24; 1083.1 ± 0.38; and 68.9 ± 0.12, respectively.Conclusions: We found that Stigmasta-4,22-dien-3-onewas the most active compound toward DPPH.Objective: P. pulchrum grows abundantly in Kendari (Sulawesi Tenggara province, Indonesia).However, there is no report neither chemical contents nor biological activitiesof the plant. This project studiesthe isolation, structure elucidations, and radical scavenging activity evaluation of triterpene steroids from stems of P. pulchrum.Methods: The isolation of the compounds was carried out by using chromatography method, i.e., vacuum liquid chromatography (VLC) and radial chromatography (RC) with silica gel as an adsorbent and various solvents as eluent. The compound structures were evaluated by spectroscopic data (FTIR and NMR data) and then the results were compared with the existing data from references. The antioxidant activity of these compounds was evaluated towards DPPH (1,1-diphenyl 2-picryl-hydrazyl).

AB - Results: Four triterpene steroids; namely, (1) 6β-hydroxystigmasta-4,22-dien-3-one, (2) stigmasterol,(3) stigmasta-4,22-dien-3-one, and (4) ergosterol peroxide, were isolated and identified from stems of P. pulchrum Bl. The antioxidant activities of all compounds were indicated by IC50 value of the compounds. The values of IC50(μM) of6β-hydroxystigmasta-4,22-dien-3-one, stigmasterol, Stigmasta-4,22-dien-3-on,ergosterol peroxide, and vitamine C (standard) toward DPPH were obtained at233.4 ± 0.28; 372.3 ± 0.33; 144.80 ± 0.24; 1083.1 ± 0.38; and 68.9 ± 0.12, respectively.Conclusions: We found that Stigmasta-4,22-dien-3-onewas the most active compound toward DPPH.Objective: P. pulchrum grows abundantly in Kendari (Sulawesi Tenggara province, Indonesia).However, there is no report neither chemical contents nor biological activitiesof the plant. This project studiesthe isolation, structure elucidations, and radical scavenging activity evaluation of triterpene steroids from stems of P. pulchrum.Methods: The isolation of the compounds was carried out by using chromatography method, i.e., vacuum liquid chromatography (VLC) and radial chromatography (RC) with silica gel as an adsorbent and various solvents as eluent. The compound structures were evaluated by spectroscopic data (FTIR and NMR data) and then the results were compared with the existing data from references. The antioxidant activity of these compounds was evaluated towards DPPH (1,1-diphenyl 2-picryl-hydrazyl).

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KW - 6β-hydroxystigmasta-4

KW - Ergosterol peroxide and DPPH

KW - P. pulchrum Bl

KW - Stigmasta-4

KW - Stigmasterol

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