RACK-1 overexpression protects against goniothalamin-induced cell death

S. H. Inayat-Hussain, L. T. Wong, Chan Kok Meng, Nor Fadilah Rajab, L. B. Din, R. Harun, A. Kizilors, N. Saxena, M. Mourtada-Maarabouni, F. Farzaneh, G. T. Williams

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Goniothalamin, a styryllactone, has been shown to induce cytotoxicity via apoptosis in several tumor cell lines. In this study, we have examined the potential role of several genes, which were stably transfected into T-cell lines and which regulate apoptosis in different ways, on goniothalamin-induced cell death. Overexpression of full-length receptor for activated protein C-kinase 1 (RACK-1) and pc3n3, which up-regulates endogenous RACK-1, in both Jurkat and W7.2 T cells resulted in inhibition of goniothalamin-induced cell death as assessed by MTT and clonogenic assays. However, overexpression of rFau (antisense sequence to Finkel-Biskis-Reilly murine sarcoma virus-associated ubiquitously expressed gene) in W7.2 cells did not confer resistance to goniothalamin-induced cell death. Etoposide, a clinically used cytotoxic agent, was equipotent in causing cytotoxicity in all the stable transfectants. Assessment of DNA damage by Comet assay revealed goniothalamin-induced DNA strand breaks as early as 1 h in vector control but this effect was inhibited in RACK-1 and pc3n3 stably transfected W7.2 cells. This data demonstrate that RACK-1 plays a crucial role in regulating cell death signalling pathways induced by goniothalamin.

Original languageEnglish
Pages (from-to)118-122
Number of pages5
JournalToxicology Letters
Volume191
Issue number2-3
DOIs
Publication statusPublished - 15 Dec 2009

Fingerprint

Cell death
Cell Death
T-cells
Cytotoxicity
Assays
Genes
Murine Sarcoma Viruses
Apoptosis
T-Lymphocytes
DNA Breaks
Comet Assay
DNA
Cytotoxins
Etoposide
Tumor Cell Line
Viruses
DNA Damage
goniothalamin
S cerevisiae ASC1 protein
Tumors

Keywords

  • Cell death
  • DNA damage
  • Goniothalamin
  • RACK-1

ASJC Scopus subject areas

  • Toxicology

Cite this

Inayat-Hussain, S. H., Wong, L. T., Kok Meng, C., Rajab, N. F., Din, L. B., Harun, R., ... Williams, G. T. (2009). RACK-1 overexpression protects against goniothalamin-induced cell death. Toxicology Letters, 191(2-3), 118-122. https://doi.org/10.1016/j.toxlet.2009.08.012

RACK-1 overexpression protects against goniothalamin-induced cell death. / Inayat-Hussain, S. H.; Wong, L. T.; Kok Meng, Chan; Rajab, Nor Fadilah; Din, L. B.; Harun, R.; Kizilors, A.; Saxena, N.; Mourtada-Maarabouni, M.; Farzaneh, F.; Williams, G. T.

In: Toxicology Letters, Vol. 191, No. 2-3, 15.12.2009, p. 118-122.

Research output: Contribution to journalArticle

Inayat-Hussain, SH, Wong, LT, Kok Meng, C, Rajab, NF, Din, LB, Harun, R, Kizilors, A, Saxena, N, Mourtada-Maarabouni, M, Farzaneh, F & Williams, GT 2009, 'RACK-1 overexpression protects against goniothalamin-induced cell death', Toxicology Letters, vol. 191, no. 2-3, pp. 118-122. https://doi.org/10.1016/j.toxlet.2009.08.012
Inayat-Hussain, S. H. ; Wong, L. T. ; Kok Meng, Chan ; Rajab, Nor Fadilah ; Din, L. B. ; Harun, R. ; Kizilors, A. ; Saxena, N. ; Mourtada-Maarabouni, M. ; Farzaneh, F. ; Williams, G. T. / RACK-1 overexpression protects against goniothalamin-induced cell death. In: Toxicology Letters. 2009 ; Vol. 191, No. 2-3. pp. 118-122.
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