Quercus infectoria gall extract enhanced the proliferation and activity of human fetal osteoblast cell line (hFOB 1.19)

Hermizi Hapidin, Dalila Rozelan, Hasmah Abdullah, Wan Nurhidayah Wan Hanaffi, Ima Nirwana Soelaiman

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Background: The present study investigated the effects of Quercus infectoria (QI) gall extract on the proliferation, alkaline phosphatase (ALP), osteocalcin, and the morphology of a human fetal osteoblast cell line (hFOB 1.19). Methods: The cells were cultured in Dulbecco’s modified eagle medium F12 supplemented with a 10% fetal bovine serum, a 1% penicillin/streptomycin and were treated with QI at various concentrations (0.1 to 99.0 μg/mL) for 72 hours. The levels of ALP and osteocalcin were measured at day 1, 3, 7, 10, and 14 and were compared among the negative control, pamidronate and QI groups. Results: The median effective concentration (EC<inf>50</inf>) of hFOB 1.19 treated with QI was 10.30 μg/mL. This concentration was more effective compared to the control drug, pamidronate (EC<inf>50</inf> at 16.09 μg/mL). The ALP and osteocalcin levels of hFOB 1.19 treated with QI from day 7 and onwards were significantly increased in a time and concentration-dependent manner. Interestingly, from day 7 until day 14, the ALP and osteocalcin levels were highest in the cells treated with QI compared to the other two groups. The morphology of cells treated with QI was uniformly elongated, higher in number and over-confluent. Conclusion: After treatment with QI, cell proliferation enhanced and ALP and osteocalcin levels increased.

Original languageEnglish
Pages (from-to)12-22
Number of pages11
JournalMalaysian Journal of Medical Sciences
Volume22
Issue number1
Publication statusPublished - 2015

Fingerprint

Quercus
Fetal Movement
Osteoblasts
Human Activities
Osteocalcin
Cell Line
pamidronate
Alkaline Phosphatase
Eagles
Drug and Narcotic Control
Streptomycin
Penicillins
Cultured Cells
Cell Proliferation

Keywords

  • Alkaline phosphatase (ALP)
  • HFOB 1.19 cell line
  • MTT assay
  • Osteocalcin
  • Quercus infectoria

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Quercus infectoria gall extract enhanced the proliferation and activity of human fetal osteoblast cell line (hFOB 1.19). / Hapidin, Hermizi; Rozelan, Dalila; Abdullah, Hasmah; Hanaffi, Wan Nurhidayah Wan; Soelaiman, Ima Nirwana.

In: Malaysian Journal of Medical Sciences, Vol. 22, No. 1, 2015, p. 12-22.

Research output: Contribution to journalArticle

Hapidin, Hermizi ; Rozelan, Dalila ; Abdullah, Hasmah ; Hanaffi, Wan Nurhidayah Wan ; Soelaiman, Ima Nirwana. / Quercus infectoria gall extract enhanced the proliferation and activity of human fetal osteoblast cell line (hFOB 1.19). In: Malaysian Journal of Medical Sciences. 2015 ; Vol. 22, No. 1. pp. 12-22.
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