Quantitative RT PCR approach to evaluate the neurogenic and gliagenic gene expression of cultured human amniotic epithelial cells.

Geok Chin Tan, S. F. Simat, H. Abdul Rahman, A. E. Tan, Chua Kien Hui

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The aim of the study is to determine the neuronal and glial gene expression of cultured human amniotic epithelial cells (HAECs) in serial passages. HAECs obtained from human term placentae were cultured in F12:DMEM (1:1) + 10% FBS +10ng/ml EGF in serial passages. Quantitative RT-PCR was used to assess the gene expression analysis. The results showed that the cultured HAECs expressed the neural stem cell genes (Nestin, NSE and Vimentin), mature neuronal genes (TH, MAP-2, beta-III-tubulin and NFM) and glial genes (CNPase, MBP and Olig). These neural stem cell genes increased with serial passages while the genes expression for mature neuronal and glial cells were downregulated. These results suggested that HAECs may promote or involve in neurogenesis and gliagenesis.

Original languageEnglish
Pages (from-to)51-52
Number of pages2
JournalThe Medical journal of Malaysia
Volume63 Suppl A
Publication statusPublished - Jul 2008

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Serial Passage
Epithelial Cells
Gene Expression
Polymerase Chain Reaction
Neuroglia
Neural Stem Cells
Genes
2',3'-Cyclic-Nucleotide Phosphodiesterases
Nestin
Neurogenesis
Vimentin
Tubulin
Epidermal Growth Factor
Placenta
Down-Regulation

ASJC Scopus subject areas

  • Medicine(all)

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Quantitative RT PCR approach to evaluate the neurogenic and gliagenic gene expression of cultured human amniotic epithelial cells. / Tan, Geok Chin; Simat, S. F.; Abdul Rahman, H.; Tan, A. E.; Kien Hui, Chua.

In: The Medical journal of Malaysia, Vol. 63 Suppl A, 07.2008, p. 51-52.

Research output: Contribution to journalArticle

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