Quantitative gas chromatographic method for the analysis of cis and trans fatty acid in margarines

Jumat Salimon, Talal Ahmed, Nadia Salih

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Fats and oils in human diets are the main sources of essential fatty acids for the body. However, there is a mounting concern about the intake of foods containing trans fatty acids (TFAs) due to their deleterious effects on human. Thus, the accurate detection of fatty acids (FAs) and TFAs is needed to control and correct nutrition labeling in dietary fat samples. Accordingly, a method for the identification and quantification of FAs and TFAs in food fats by gas chromatography (GC) based on the extraction of lipids and derivatization using base catalyzed followed by trimethylsilyl-diazomethane (TMS-DM) was developed. The proposed method was evaluated to standard mixture of oleic acid (OA) (C18:1 cis 9) and Elaidic acid (EA) (C18:1 trans 9) and its application to three samples of commercial margarines was demonstrated. Based on the results obtained, recovery values (R) from all the samples were close to 100%. Repeatability (RSD) values ranged between 0.78 and 2.47%, while Reproducibility (RSD) values ranged between 1.14 and 3.65%. Consequently, the proposed method is sensitive, accurate and suitable for FAs and TFAs analysis of food fats and oils and can be applied to nutritional, medicine and food studies.

Original languageEnglish
Pages (from-to)1937-1942
Number of pages6
JournalSains Malaysiana
Volume43
Issue number12
Publication statusPublished - 1 Dec 2014

Fingerprint

Trans Fatty Acids
Gases
Fatty acids
Nutrition
Oils and fats
Diazomethane
Essential Fatty Acids
Dietary Fats
Oleic Acid
Mountings
Gas chromatography
Labeling
Medicine
Fats
Lipids
Recovery

Keywords

  • Derivatization
  • Food fats
  • Quantitative analyses
  • Trans fatty acids

ASJC Scopus subject areas

  • General

Cite this

Quantitative gas chromatographic method for the analysis of cis and trans fatty acid in margarines. / Salimon, Jumat; Ahmed, Talal; Salih, Nadia.

In: Sains Malaysiana, Vol. 43, No. 12, 01.12.2014, p. 1937-1942.

Research output: Contribution to journalArticle

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