Quantitative evaluation of cell death response in vitro and in vivo using conventional-frequency ultrasound

Ali Sadeghi-Naini, Stephanie Zhou, Mehrdad J. Gangeh, Zahra Jahedmotlagh, Omar Falou, Shawn Ranieri, Muhammad Azrif, Anoja Giles, Gregory J. Czarnota

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Previous studies using high-frequency ultrasound have suggested that radiofrequency (RF) spectral analysis can be used to quantify changes in cell morphology to detect cell death response to therapy non-invasively. The study here investigated this at conventional-frequencies, frequently used in clinical settings. Spectral analysis was performed using ultrasound RF data collected with a clinical ultrasound platform. Acute myeloid leukemia (AML-5) cells were exposed to cisplatinum for 0-72 hours in vitro and prepared for ultrasound data collection. Preclinical in vivo experiments were also performed on AML-5 tumour-bearing mice receiving chemotherapy. The mid-band fit (MBF) spectral parameter demonstrated an increase of 4.4 ± 1.5 dBr for in vitro samples assessed 48 hours after treatment, a statistically significant change (p <0.05) compared to control. Further, in vitro concentration-based analysis of a mixture of apoptotic and untreated cells indicated a mean change of 10.9 ± 2.4 dBr in MBF between 0% and 40% apoptotic cell mixtures. Similar effects were reproduced in vivo with an increase of 4.6 ± 0.3 dBr in MBF compared to control, for tumours with considerable apoptotic areas within histological samples. The alterations in the size of cells and nuclei corresponded well with changes measured in the quantitative ultrasound (QUS) parameters.

Original languageEnglish
Pages (from-to)716-726
Number of pages11
JournalOncoscience
Volume2
Issue number8
DOIs
Publication statusPublished - 2015
Externally publishedYes

Fingerprint

Cell Death
Cell Nucleus Size
Acute Myeloid Leukemia
Neoplasms
Drug Therapy
In Vitro Techniques
Therapeutics

Keywords

  • Apoptosis
  • Cancer therapy
  • Personalized medicine
  • Quantitative ultrasound
  • Treatment response monitoring

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Sadeghi-Naini, A., Zhou, S., Gangeh, M. J., Jahedmotlagh, Z., Falou, O., Ranieri, S., ... Czarnota, G. J. (2015). Quantitative evaluation of cell death response in vitro and in vivo using conventional-frequency ultrasound. Oncoscience, 2(8), 716-726. https://doi.org/10.18632/oncoscience.235

Quantitative evaluation of cell death response in vitro and in vivo using conventional-frequency ultrasound. / Sadeghi-Naini, Ali; Zhou, Stephanie; Gangeh, Mehrdad J.; Jahedmotlagh, Zahra; Falou, Omar; Ranieri, Shawn; Azrif, Muhammad; Giles, Anoja; Czarnota, Gregory J.

In: Oncoscience, Vol. 2, No. 8, 2015, p. 716-726.

Research output: Contribution to journalArticle

Sadeghi-Naini, A, Zhou, S, Gangeh, MJ, Jahedmotlagh, Z, Falou, O, Ranieri, S, Azrif, M, Giles, A & Czarnota, GJ 2015, 'Quantitative evaluation of cell death response in vitro and in vivo using conventional-frequency ultrasound', Oncoscience, vol. 2, no. 8, pp. 716-726. https://doi.org/10.18632/oncoscience.235
Sadeghi-Naini, Ali ; Zhou, Stephanie ; Gangeh, Mehrdad J. ; Jahedmotlagh, Zahra ; Falou, Omar ; Ranieri, Shawn ; Azrif, Muhammad ; Giles, Anoja ; Czarnota, Gregory J. / Quantitative evaluation of cell death response in vitro and in vivo using conventional-frequency ultrasound. In: Oncoscience. 2015 ; Vol. 2, No. 8. pp. 716-726.
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