Analisis kuantitatif bagi sebatian fenolik dalam dua varieti roselle (Hibiscus sabdariffa) menggunakan kromatografi cecair berprestasi tinggi

Translated title of the contribution: Quantitative analysis of phenolics content in two roselle varieties (Hibiscus sabdariffa) by high performance liquid chromatography

Siti Aishah Mohd Ali, Satirah Zainalabidin, Jalifah Latip

Research output: Contribution to journalArticle

Abstract

Phenolics content of two roselle varieties, H. sabdariffa var. UKMR-2 and H. sabdariffa var. UMKL-1 was determined based on high performance liquid chromatography method using a photodiode array detector (HPLC-PDA) with gradient elution. Roselle calyces were extracted with water via sonication (50°C, 30 minutes). The chromatographic separation was carried out on Purospher STAR RP-18e LichroCART column (250 mm × 4.6 mm × 5 µm) and detection was carried out at three different wavelengths (265, 320 and 520 nm). The developed HPLC method provided high percentage of recovery (95-102%), good linearity at R2 > 0.99, low limit of detection (LOD) and low limit of quantitation (LOQ). Two predominant anthocyanins were detected by HPLC-PDA in both H. sabdariffa varieties and were identified as delphinidin-3-O-sambubioside and cyanidin-3-O-sambubioside. In addition, chlorogenic acid, caffeic acid and ascorbic acid were also detected. High content of phenolics and ascorbic acid were detected in UKMR-2 as compared to UMKL-1, suggested this variety have high potential for commercial cultivation.

Original languageMalay
Pages (from-to)715-724
Number of pages10
JournalMalaysian Journal of Analytical Sciences
Volume23
Issue number4
DOIs
Publication statusPublished - 1 Aug 2019

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delphinidin
Personal digital assistants
High performance liquid chromatography
Ascorbic Acid
Chlorogenic Acid
Sonication
Anthocyanins
Photodiodes
Chemical analysis
Detectors
Recovery
Wavelength
Water
caffeic acid
phenolic acid
cyanidin-3-O-sambubioside

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

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title = "Analisis kuantitatif bagi sebatian fenolik dalam dua varieti roselle (Hibiscus sabdariffa) menggunakan kromatografi cecair berprestasi tinggi",
abstract = "Phenolics content of two roselle varieties, H. sabdariffa var. UKMR-2 and H. sabdariffa var. UMKL-1 was determined based on high performance liquid chromatography method using a photodiode array detector (HPLC-PDA) with gradient elution. Roselle calyces were extracted with water via sonication (50°C, 30 minutes). The chromatographic separation was carried out on Purospher STAR RP-18e LichroCART column (250 mm × 4.6 mm × 5 µm) and detection was carried out at three different wavelengths (265, 320 and 520 nm). The developed HPLC method provided high percentage of recovery (95-102{\%}), good linearity at R2 > 0.99, low limit of detection (LOD) and low limit of quantitation (LOQ). Two predominant anthocyanins were detected by HPLC-PDA in both H. sabdariffa varieties and were identified as delphinidin-3-O-sambubioside and cyanidin-3-O-sambubioside. In addition, chlorogenic acid, caffeic acid and ascorbic acid were also detected. High content of phenolics and ascorbic acid were detected in UKMR-2 as compared to UMKL-1, suggested this variety have high potential for commercial cultivation.",
keywords = "Anthocyanins, Hibiscus sabdariffa, HPLC-PDA",
author = "Ali, {Siti Aishah Mohd} and Satirah Zainalabidin and Jalifah Latip",
year = "2019",
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AU - Ali, Siti Aishah Mohd

AU - Zainalabidin, Satirah

AU - Latip, Jalifah

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AB - Phenolics content of two roselle varieties, H. sabdariffa var. UKMR-2 and H. sabdariffa var. UMKL-1 was determined based on high performance liquid chromatography method using a photodiode array detector (HPLC-PDA) with gradient elution. Roselle calyces were extracted with water via sonication (50°C, 30 minutes). The chromatographic separation was carried out on Purospher STAR RP-18e LichroCART column (250 mm × 4.6 mm × 5 µm) and detection was carried out at three different wavelengths (265, 320 and 520 nm). The developed HPLC method provided high percentage of recovery (95-102%), good linearity at R2 > 0.99, low limit of detection (LOD) and low limit of quantitation (LOQ). Two predominant anthocyanins were detected by HPLC-PDA in both H. sabdariffa varieties and were identified as delphinidin-3-O-sambubioside and cyanidin-3-O-sambubioside. In addition, chlorogenic acid, caffeic acid and ascorbic acid were also detected. High content of phenolics and ascorbic acid were detected in UKMR-2 as compared to UMKL-1, suggested this variety have high potential for commercial cultivation.

KW - Anthocyanins

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