Qualitative and quantitative phytochemical analysis and antioxidant properties of leaves and stems of clinacanthus nutans (Burm. f.) lindau from two herbal farms of Negeri Sembilan, Malaysia

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Abstract

The objective of the study is to analyse the phytochemical content by quantitative and qualitative methods and to investigate the antioxidant properties on Clinacanthus nutans leaves and stems from different areas: Yik Poh Ling (YPL) un-shaded sample (exposed to direct sunlight) and You Dun Chao (YDC) un-shaded and shaded (with black shade netting) samples. Extracts were prepared by dissolving fine powder of C. nutans in 1:10 solvent using ultrasonic extraction for an hour. Preliminary screening and quantitative estimation of phytochemical (alkaloids, flavonoids, cardiac glycosides, saponins, steroids, terpenoids, phenols and tannins) analysis were conducted. Then, determination of antioxidant properties (total phenolic content, TPC, ferric reducing/ antioxidant power, FRAP and radical-scavenging activities, DPPH) were conducted. All the samples contained alkaloids, flavonoids, cardiac glycosides, saponins, steroids, terpenoids, phenols and tannins. Un-shaded leaves of C. nutans from YDC exhibited significantly higher result in antioxidant properties (966.00mg GAE/g of dried sample in TPC, 20.44mg TE/g of dried sample in FRAP and 11.14mg TE/g of dried sample in DPPH) than the other samples (p < 0.05). As a conclusion, C. nutans has a good potential to be an alternative antioxidant source.

Original languageEnglish
JournalASM Science Journal
Volume12
DOIs
Publication statusPublished - 1 Jan 2019

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Malaysia
phytopharmaceuticals
antioxidants
farms
stems
leaves
cardiac glycosides
steroid saponins
sampling
terpenoids
phenols
tannins
alkaloids
flavonoids
netting
qualitative analysis
Clinacanthus nutans
quantitative analysis
ultrasonics
powders

Keywords

  • Antioxidant
  • Clinacanthus nutans
  • Phytochemical
  • Sabah Snake Grass
  • Shaded and un-shaded

ASJC Scopus subject areas

  • General

Cite this

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title = "Qualitative and quantitative phytochemical analysis and antioxidant properties of leaves and stems of clinacanthus nutans (Burm. f.) lindau from two herbal farms of Negeri Sembilan, Malaysia",
abstract = "The objective of the study is to analyse the phytochemical content by quantitative and qualitative methods and to investigate the antioxidant properties on Clinacanthus nutans leaves and stems from different areas: Yik Poh Ling (YPL) un-shaded sample (exposed to direct sunlight) and You Dun Chao (YDC) un-shaded and shaded (with black shade netting) samples. Extracts were prepared by dissolving fine powder of C. nutans in 1:10 solvent using ultrasonic extraction for an hour. Preliminary screening and quantitative estimation of phytochemical (alkaloids, flavonoids, cardiac glycosides, saponins, steroids, terpenoids, phenols and tannins) analysis were conducted. Then, determination of antioxidant properties (total phenolic content, TPC, ferric reducing/ antioxidant power, FRAP and radical-scavenging activities, DPPH) were conducted. All the samples contained alkaloids, flavonoids, cardiac glycosides, saponins, steroids, terpenoids, phenols and tannins. Un-shaded leaves of C. nutans from YDC exhibited significantly higher result in antioxidant properties (966.00mg GAE/g of dried sample in TPC, 20.44mg TE/g of dried sample in FRAP and 11.14mg TE/g of dried sample in DPPH) than the other samples (p < 0.05). As a conclusion, C. nutans has a good potential to be an alternative antioxidant source.",
keywords = "Antioxidant, Clinacanthus nutans, Phytochemical, Sabah Snake Grass, Shaded and un-shaded",
author = "Kong, {H. S.} and Musa, {K. H.} and {Mohd Kasim}, Zalifah and {Abdullah Sani}, Norrakiah",
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AU - Musa, K. H.

AU - Mohd Kasim, Zalifah

AU - Abdullah Sani, Norrakiah

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N2 - The objective of the study is to analyse the phytochemical content by quantitative and qualitative methods and to investigate the antioxidant properties on Clinacanthus nutans leaves and stems from different areas: Yik Poh Ling (YPL) un-shaded sample (exposed to direct sunlight) and You Dun Chao (YDC) un-shaded and shaded (with black shade netting) samples. Extracts were prepared by dissolving fine powder of C. nutans in 1:10 solvent using ultrasonic extraction for an hour. Preliminary screening and quantitative estimation of phytochemical (alkaloids, flavonoids, cardiac glycosides, saponins, steroids, terpenoids, phenols and tannins) analysis were conducted. Then, determination of antioxidant properties (total phenolic content, TPC, ferric reducing/ antioxidant power, FRAP and radical-scavenging activities, DPPH) were conducted. All the samples contained alkaloids, flavonoids, cardiac glycosides, saponins, steroids, terpenoids, phenols and tannins. Un-shaded leaves of C. nutans from YDC exhibited significantly higher result in antioxidant properties (966.00mg GAE/g of dried sample in TPC, 20.44mg TE/g of dried sample in FRAP and 11.14mg TE/g of dried sample in DPPH) than the other samples (p < 0.05). As a conclusion, C. nutans has a good potential to be an alternative antioxidant source.

AB - The objective of the study is to analyse the phytochemical content by quantitative and qualitative methods and to investigate the antioxidant properties on Clinacanthus nutans leaves and stems from different areas: Yik Poh Ling (YPL) un-shaded sample (exposed to direct sunlight) and You Dun Chao (YDC) un-shaded and shaded (with black shade netting) samples. Extracts were prepared by dissolving fine powder of C. nutans in 1:10 solvent using ultrasonic extraction for an hour. Preliminary screening and quantitative estimation of phytochemical (alkaloids, flavonoids, cardiac glycosides, saponins, steroids, terpenoids, phenols and tannins) analysis were conducted. Then, determination of antioxidant properties (total phenolic content, TPC, ferric reducing/ antioxidant power, FRAP and radical-scavenging activities, DPPH) were conducted. All the samples contained alkaloids, flavonoids, cardiac glycosides, saponins, steroids, terpenoids, phenols and tannins. Un-shaded leaves of C. nutans from YDC exhibited significantly higher result in antioxidant properties (966.00mg GAE/g of dried sample in TPC, 20.44mg TE/g of dried sample in FRAP and 11.14mg TE/g of dried sample in DPPH) than the other samples (p < 0.05). As a conclusion, C. nutans has a good potential to be an alternative antioxidant source.

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