Purification and properties of a new dehalogenase enzyme from Pseudomonas sp. B6P grow in 3-chloropropionate (3CP)

T. H T Abdul Hamid, A. A A Hamid, A. H. Zulkifly, S. Hamdan, Shahrul Hisham Zainal Ariffin, F. Huyop

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Halogenated compounds are widely used in agriculture and industries and have been associated with environmental pollution. Degradation of 3-chloropropionate (3CP) by microorganism has been established and this enzyme could only remove halogen atom at the β- position of 3-carbon alkanoic acids. Pseudomonas sp. B6P was originally isolated from paddy field which was able to degrade 3CP therefore, suggesting it may have some desirable properties. The enzyme was purified from cell-free extracts having a monomer of 56,000 Da. It was found to be stable between pH 5 to 8 and its optimal pH was 8. Its activity was not affected by metal ions such as Mn2+, Fe3+ and Mg2+, but was inhibited by Hg2+ and Ag2+. The enzyme is specific for 3CP, and the Km value (0.20 mM ± 0.05).

Original languageEnglish
Pages (from-to)610-614
Number of pages5
JournalAfrican Journal of Biotechnology
Volume10
Issue number4
Publication statusPublished - 24 Jan 2011

Fingerprint

Pseudomonas
Enzymes
enzymes
halogens
Environmental Pollution
Halogens
Cell Extracts
Agriculture
metal ions
paddies
Industry
Carbon
pollution
Metals
Ions
agriculture
industry
microorganisms
Acids
degradation

Keywords

  • 3-chloropropionic acid
  • Biodegradation
  • Bioremediation
  • Dehalogenase
  • Haloalkanoic acid
  • Pseudomonas sp.B6P

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Genetics
  • Molecular Biology
  • Agronomy and Crop Science

Cite this

Purification and properties of a new dehalogenase enzyme from Pseudomonas sp. B6P grow in 3-chloropropionate (3CP). / Abdul Hamid, T. H T; Hamid, A. A A; Zulkifly, A. H.; Hamdan, S.; Zainal Ariffin, Shahrul Hisham; Huyop, F.

In: African Journal of Biotechnology, Vol. 10, No. 4, 24.01.2011, p. 610-614.

Research output: Contribution to journalArticle

Abdul Hamid, T. H T ; Hamid, A. A A ; Zulkifly, A. H. ; Hamdan, S. ; Zainal Ariffin, Shahrul Hisham ; Huyop, F. / Purification and properties of a new dehalogenase enzyme from Pseudomonas sp. B6P grow in 3-chloropropionate (3CP). In: African Journal of Biotechnology. 2011 ; Vol. 10, No. 4. pp. 610-614.
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AU - Zainal Ariffin, Shahrul Hisham

AU - Huyop, F.

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