Purification and polymerisation of microbial D-lactic acid from DDGS hydrolysates fermentation

Nurul Aqilah Mohd Zaini, Afroditi Chatzifragkou, Viacheslav Tverezovskiy, Dimitris Charalampopoulos

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A multi-step process was developed for microbial D-lactic acid purification, followed by poly-D-lactic acid (PDLA) synthesis via azeotropic polycondensation process. Several anion exchange resins were screened for their binding capacity using model lactic acid solutions. Amberlite® IRA67 (weak base anion exchange resin) showed the highest lactic acid adsorption, with maximum adsorption capacity, qmax, of 136.11 mg lactic acid / g of resin, and was further selected to purify D-lactic acid from DDGS hydrolysates through a three-step process; (1) treatment with 7% w/v activated carbon, (2) acidification of fermentation broth (Amberlite® IRA120) and (3) adsorption of lactic acid by anion exchange (Amberlite® IRA67). At the end of the purification process, 80.4% (w/w) D-lactic acid was recovered with 91.8% (w/w) purity, indicating the effectiveness of the developed downstream process. Furthermore, a clear yellowish solid polymer with a molecular weight of 3010 Da was obtained, suitable for applications in biomedical and agricultural sectors.

Original languageEnglish
Article number107265
JournalBiochemical Engineering Journal
Volume150
DOIs
Publication statusPublished - 15 Oct 2019

Fingerprint

Lactic acid
Polymerization
Fermentation
Purification
Lactic Acid
Anion Exchange Resins
Adsorption
Ion exchange
Negative ions
Resins
Acidification
Polycondensation
Activated carbon
Anions
Polymers
Carbon
Molecular Weight
Molecular weight
amberlite

Keywords

  • Activated carbon
  • DDGS
  • Ion exchange resin
  • Poly-D-lactic acid
  • polycondensation
  • Purification

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Environmental Engineering
  • Biomedical Engineering

Cite this

Purification and polymerisation of microbial D-lactic acid from DDGS hydrolysates fermentation. / Mohd Zaini, Nurul Aqilah; Chatzifragkou, Afroditi; Tverezovskiy, Viacheslav; Charalampopoulos, Dimitris.

In: Biochemical Engineering Journal, Vol. 150, 107265, 15.10.2019.

Research output: Contribution to journalArticle

Mohd Zaini, Nurul Aqilah ; Chatzifragkou, Afroditi ; Tverezovskiy, Viacheslav ; Charalampopoulos, Dimitris. / Purification and polymerisation of microbial D-lactic acid from DDGS hydrolysates fermentation. In: Biochemical Engineering Journal. 2019 ; Vol. 150.
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