Purification and identification of ACE inhibitory peptides from Haruan (Channa striatus) myofibrillar protein hydrolysate using HPLC-ESI-TOF MS/MS

Masomeh Ghassem, Keizo Arihara, Abd. Salam Babji, Mamot Said, Saadiah Ibrahim

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Haruan myofibrillar protein was hydrolysed with proteinase K and thermolysin to isolate Angiotensin converting enzyme (ACE) inhibitory peptides. The thermolysin hydrolysate of myofibrillar protein with the highest ACE inhibition activity (IC50 = 0.033 mg/ml) was fractionated by ultrafiltration and size exclusion chromatography to three fractions. Fraction F2 with higher ACE inhibitory activity was separated into five fractions (A-E) using reversed-phased high performance liquid chromatography (RP-HPLC). Fraction C showed 81% inhibition activity and was subjected to HPLC coupled to electrospray ionisation-time-of-flight mass spectrometry (ESI-TOF MS/MS). Two peptide sequences for the most abundant fragments were identified as VPAAPPK (IC50 = 0.45 μM) at 791.155 m/z and NGTWFEPP (IC50 = 0.63 μM) at 1085.841 m/z. The presence of two proline residues at the C-terminal sequence is responsible for the high ACE inhibitory activity of these peptides. The results suggest that Haruan meat protein hydrolysate is a potent ACE inhibitor and may be used to decrease blood pressure.

Original languageEnglish
Pages (from-to)1770-1777
Number of pages8
JournalFood Chemistry
Volume129
Issue number4
DOIs
Publication statusPublished - 15 Dec 2011

Fingerprint

Channa
Protein Hydrolysates
myofibrillar proteins
protein hydrolysates
peptidyl-dipeptidase A
Peptidyl-Dipeptidase A
Purification
high performance liquid chromatography
High Pressure Liquid Chromatography
peptides
Thermolysin
Inhibitory Concentration 50
inhibitory concentration 50
Peptides
Enzyme activity
Enzyme inhibition
meat protein
Endopeptidase K
Electrospray ionization
Size exclusion chromatography

Keywords

  • ACE-inhibitory activity
  • Electrospray ionisation
  • Mass spectrometry
  • Quadropole-time of flight
  • Thermolysin hydrolysate

ASJC Scopus subject areas

  • Food Science
  • Analytical Chemistry

Cite this

Purification and identification of ACE inhibitory peptides from Haruan (Channa striatus) myofibrillar protein hydrolysate using HPLC-ESI-TOF MS/MS. / Ghassem, Masomeh; Arihara, Keizo; Babji, Abd. Salam; Said, Mamot; Ibrahim, Saadiah.

In: Food Chemistry, Vol. 129, No. 4, 15.12.2011, p. 1770-1777.

Research output: Contribution to journalArticle

Ghassem, Masomeh ; Arihara, Keizo ; Babji, Abd. Salam ; Said, Mamot ; Ibrahim, Saadiah. / Purification and identification of ACE inhibitory peptides from Haruan (Channa striatus) myofibrillar protein hydrolysate using HPLC-ESI-TOF MS/MS. In: Food Chemistry. 2011 ; Vol. 129, No. 4. pp. 1770-1777.
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