Pure tocotrienol concentrate protected rat gastric mucosa from acute stress-induced injury by a non-antioxidant mechanism

Mohd Nor Syidiq Rodzian, Ibrahim Abdel Aziz Ibrahim, Nur Azlina Mohd Fahami, Nafeeza Mohd Ismail

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Stress has been implicated as a risk factor of various major health problems, such as stress-induced gastric mucosal injury. This study was performed to investigate the action of a pure preparation of tocotrienol (T3) concentrate, made up of 90% δ -tocotrienol and 10% γ -tocotrienol, on gastric injury of rats induced by water-immersion restraint stress (WIRS). Fourteen male Sprague-Dawley rats (200-250 g) were divided into two equal groups: a control group and a treated group. The treatment group received T3 concentrate at 60 mg/kg body weight daily for 28 days. The body weights of rats were recorded daily before the treatment was given. At the end of the treatment period, all rats were subjected to WIRS for 3.5 hours, following which the rats were euthanized. The stomachs were isolated and opened along the greater curvature for the examination of lesions and measurements of gastric malondialdehyde (MDA) and prostaglandin E2 (PGE2) contents. The mean gastric mucosal lesion index in the treated rats was significantly lower than that in the control rats. This suggests that the T3 concentrate has the ability to confer protection to the gastric mucosa against gastric injury induced by acute stress. No significant difference was observed for changes in body weight before and after the treatment. The gastric PGE2 content in both groups was comparable. However, the gastric MDA content was significantly higher in the treated group compared to the control group, indicating that the T3 supplementation was not able to reduce the lipid peroxidation process. This study concludes that the T3 concentrate has the ability to protect the gastric mucosa from stress-induced injury by a non-antioxidant mechanism.

Original languageEnglish
Pages (from-to)52-58
Number of pages7
JournalPolish Journal of Pathology
Volume64
Issue number1
DOIs
Publication statusPublished - 2013

Fingerprint

Tocotrienols
Gastric Mucosa
Stomach
Wounds and Injuries
Immersion
Malondialdehyde
Dinoprostone
Body Weight
Control Groups
Gastrointestinal Contents
Body Weight Changes
Water
Lipid Peroxidation
Sprague Dawley Rats
Health

Keywords

  • Gastric injury
  • Malondialdehyde
  • Prostaglandin E
  • Tocotrienol
  • Water-immersion restraint stress

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Pure tocotrienol concentrate protected rat gastric mucosa from acute stress-induced injury by a non-antioxidant mechanism. / Rodzian, Mohd Nor Syidiq; Aziz Ibrahim, Ibrahim Abdel; Mohd Fahami, Nur Azlina; Ismail, Nafeeza Mohd.

In: Polish Journal of Pathology, Vol. 64, No. 1, 2013, p. 52-58.

Research output: Contribution to journalArticle

Rodzian, Mohd Nor Syidiq ; Aziz Ibrahim, Ibrahim Abdel ; Mohd Fahami, Nur Azlina ; Ismail, Nafeeza Mohd. / Pure tocotrienol concentrate protected rat gastric mucosa from acute stress-induced injury by a non-antioxidant mechanism. In: Polish Journal of Pathology. 2013 ; Vol. 64, No. 1. pp. 52-58.
@article{c82b16ff2dc244488d857b68fea6f620,
title = "Pure tocotrienol concentrate protected rat gastric mucosa from acute stress-induced injury by a non-antioxidant mechanism",
abstract = "Stress has been implicated as a risk factor of various major health problems, such as stress-induced gastric mucosal injury. This study was performed to investigate the action of a pure preparation of tocotrienol (T3) concentrate, made up of 90{\%} δ -tocotrienol and 10{\%} γ -tocotrienol, on gastric injury of rats induced by water-immersion restraint stress (WIRS). Fourteen male Sprague-Dawley rats (200-250 g) were divided into two equal groups: a control group and a treated group. The treatment group received T3 concentrate at 60 mg/kg body weight daily for 28 days. The body weights of rats were recorded daily before the treatment was given. At the end of the treatment period, all rats were subjected to WIRS for 3.5 hours, following which the rats were euthanized. The stomachs were isolated and opened along the greater curvature for the examination of lesions and measurements of gastric malondialdehyde (MDA) and prostaglandin E2 (PGE2) contents. The mean gastric mucosal lesion index in the treated rats was significantly lower than that in the control rats. This suggests that the T3 concentrate has the ability to confer protection to the gastric mucosa against gastric injury induced by acute stress. No significant difference was observed for changes in body weight before and after the treatment. The gastric PGE2 content in both groups was comparable. However, the gastric MDA content was significantly higher in the treated group compared to the control group, indicating that the T3 supplementation was not able to reduce the lipid peroxidation process. This study concludes that the T3 concentrate has the ability to protect the gastric mucosa from stress-induced injury by a non-antioxidant mechanism.",
keywords = "Gastric injury, Malondialdehyde, Prostaglandin E, Tocotrienol, Water-immersion restraint stress",
author = "Rodzian, {Mohd Nor Syidiq} and {Aziz Ibrahim}, {Ibrahim Abdel} and {Mohd Fahami}, {Nur Azlina} and Ismail, {Nafeeza Mohd}",
year = "2013",
doi = "10.5114/PJP.2013.34604",
language = "English",
volume = "64",
pages = "52--58",
journal = "Polish Journal of Pathology",
issn = "1233-9687",
publisher = "Versalius University Medical Publisher",
number = "1",

}

TY - JOUR

T1 - Pure tocotrienol concentrate protected rat gastric mucosa from acute stress-induced injury by a non-antioxidant mechanism

AU - Rodzian, Mohd Nor Syidiq

AU - Aziz Ibrahim, Ibrahim Abdel

AU - Mohd Fahami, Nur Azlina

AU - Ismail, Nafeeza Mohd

PY - 2013

Y1 - 2013

N2 - Stress has been implicated as a risk factor of various major health problems, such as stress-induced gastric mucosal injury. This study was performed to investigate the action of a pure preparation of tocotrienol (T3) concentrate, made up of 90% δ -tocotrienol and 10% γ -tocotrienol, on gastric injury of rats induced by water-immersion restraint stress (WIRS). Fourteen male Sprague-Dawley rats (200-250 g) were divided into two equal groups: a control group and a treated group. The treatment group received T3 concentrate at 60 mg/kg body weight daily for 28 days. The body weights of rats were recorded daily before the treatment was given. At the end of the treatment period, all rats were subjected to WIRS for 3.5 hours, following which the rats were euthanized. The stomachs were isolated and opened along the greater curvature for the examination of lesions and measurements of gastric malondialdehyde (MDA) and prostaglandin E2 (PGE2) contents. The mean gastric mucosal lesion index in the treated rats was significantly lower than that in the control rats. This suggests that the T3 concentrate has the ability to confer protection to the gastric mucosa against gastric injury induced by acute stress. No significant difference was observed for changes in body weight before and after the treatment. The gastric PGE2 content in both groups was comparable. However, the gastric MDA content was significantly higher in the treated group compared to the control group, indicating that the T3 supplementation was not able to reduce the lipid peroxidation process. This study concludes that the T3 concentrate has the ability to protect the gastric mucosa from stress-induced injury by a non-antioxidant mechanism.

AB - Stress has been implicated as a risk factor of various major health problems, such as stress-induced gastric mucosal injury. This study was performed to investigate the action of a pure preparation of tocotrienol (T3) concentrate, made up of 90% δ -tocotrienol and 10% γ -tocotrienol, on gastric injury of rats induced by water-immersion restraint stress (WIRS). Fourteen male Sprague-Dawley rats (200-250 g) were divided into two equal groups: a control group and a treated group. The treatment group received T3 concentrate at 60 mg/kg body weight daily for 28 days. The body weights of rats were recorded daily before the treatment was given. At the end of the treatment period, all rats were subjected to WIRS for 3.5 hours, following which the rats were euthanized. The stomachs were isolated and opened along the greater curvature for the examination of lesions and measurements of gastric malondialdehyde (MDA) and prostaglandin E2 (PGE2) contents. The mean gastric mucosal lesion index in the treated rats was significantly lower than that in the control rats. This suggests that the T3 concentrate has the ability to confer protection to the gastric mucosa against gastric injury induced by acute stress. No significant difference was observed for changes in body weight before and after the treatment. The gastric PGE2 content in both groups was comparable. However, the gastric MDA content was significantly higher in the treated group compared to the control group, indicating that the T3 supplementation was not able to reduce the lipid peroxidation process. This study concludes that the T3 concentrate has the ability to protect the gastric mucosa from stress-induced injury by a non-antioxidant mechanism.

KW - Gastric injury

KW - Malondialdehyde

KW - Prostaglandin E

KW - Tocotrienol

KW - Water-immersion restraint stress

UR - http://www.scopus.com/inward/record.url?scp=84876973317&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84876973317&partnerID=8YFLogxK

U2 - 10.5114/PJP.2013.34604

DO - 10.5114/PJP.2013.34604

M3 - Article

C2 - 23625601

AN - SCOPUS:84876973317

VL - 64

SP - 52

EP - 58

JO - Polish Journal of Pathology

JF - Polish Journal of Pathology

SN - 1233-9687

IS - 1

ER -