Abstract
Methicillin-resistant Staphylococcus aureus (MRSA) is a deadly pathogen that initially was limited to hospital and healthcare facilities but has gradually became a growing problem in healthy children and adults. Pterostilbene belongs to the phenylpropanoid phytoalexin which is involved in plant response to various pathogen and herbivores attack. The aim of this study is to evaluate the anti-MRSA action of pterostilbene in combination with selected antibiotics; vancomycin, linezolid and oxacillin against ATCC 43300 and ATCC 33591. The minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and fractional inhibitory concentration (FIC) index values were determined. Microbroth dilution technique and microdilution checkerboard (MDC) assay were employed. The MIC and MBC of pterostilbene against ATCC 33591 was 31.25 and 62.50 µg mL-1, respectively. While for ATCC 43300, the MBC value was also twice (62.50 µg mL-1) its MIC value of 31.25 µg mL-1. This indicated that pterostilbene was bacteriostatic against both MRSA strains. Our MIC/MBC study also showed that linezolid exhibited bacteriostatic action but, oxacillin and vancomycin were bactericidal. MDC study showed that pterostilbene-oxacillin combination exhibited lowest FIC value (0.56) against both MRSA strains which indicated partial synergistic interaction. On the other hand, pterostilbene was additive (FIC 1.00) in combination with vancomycin whereas pterostilbene-linezolid combination displayed indifference effect with FIC of 1.25 against both MRSA strains. Pterostilbene in combination with oxacillin partially enhanced anti-MRSA activity with twofold reduction in MIC of oxacillin by acting at different site at the bacterial cell wall from that of oxacillin but more specific to the site of action of vancomycin.
| Original language | English |
|---|---|
| Pages (from-to) | 1-10 |
| Number of pages | 10 |
| Journal | Revista Brasileira de Gestao e Desenvolvimento Regional |
| Volume | 12 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2016 |
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Keywords
- Bacteriostatic
- FIC
- MIC
- MRSA
- Pterostilbene
- Synergism
ASJC Scopus subject areas
- Sociology and Political Science
- Geography, Planning and Development
- Urban Studies
- Environmental Science (miscellaneous)
Cite this
Pterostilbene enhanced anti-methicillin resistant staphylococcus aureus (MRSA) activity of oxacillin. / Ishak, Siti Fairuz; Ghazali, Ahmad Rohi; Zin, Noraziah Mohamad; Basri, Dayang Fredalina.
In: Revista Brasileira de Gestao e Desenvolvimento Regional, Vol. 12, No. 1, 2016, p. 1-10.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Pterostilbene enhanced anti-methicillin resistant staphylococcus aureus (MRSA) activity of oxacillin
AU - Ishak, Siti Fairuz
AU - Ghazali, Ahmad Rohi
AU - Zin, Noraziah Mohamad
AU - Basri, Dayang Fredalina
PY - 2016
Y1 - 2016
N2 - Methicillin-resistant Staphylococcus aureus (MRSA) is a deadly pathogen that initially was limited to hospital and healthcare facilities but has gradually became a growing problem in healthy children and adults. Pterostilbene belongs to the phenylpropanoid phytoalexin which is involved in plant response to various pathogen and herbivores attack. The aim of this study is to evaluate the anti-MRSA action of pterostilbene in combination with selected antibiotics; vancomycin, linezolid and oxacillin against ATCC 43300 and ATCC 33591. The minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and fractional inhibitory concentration (FIC) index values were determined. Microbroth dilution technique and microdilution checkerboard (MDC) assay were employed. The MIC and MBC of pterostilbene against ATCC 33591 was 31.25 and 62.50 µg mL-1, respectively. While for ATCC 43300, the MBC value was also twice (62.50 µg mL-1) its MIC value of 31.25 µg mL-1. This indicated that pterostilbene was bacteriostatic against both MRSA strains. Our MIC/MBC study also showed that linezolid exhibited bacteriostatic action but, oxacillin and vancomycin were bactericidal. MDC study showed that pterostilbene-oxacillin combination exhibited lowest FIC value (0.56) against both MRSA strains which indicated partial synergistic interaction. On the other hand, pterostilbene was additive (FIC 1.00) in combination with vancomycin whereas pterostilbene-linezolid combination displayed indifference effect with FIC of 1.25 against both MRSA strains. Pterostilbene in combination with oxacillin partially enhanced anti-MRSA activity with twofold reduction in MIC of oxacillin by acting at different site at the bacterial cell wall from that of oxacillin but more specific to the site of action of vancomycin.
AB - Methicillin-resistant Staphylococcus aureus (MRSA) is a deadly pathogen that initially was limited to hospital and healthcare facilities but has gradually became a growing problem in healthy children and adults. Pterostilbene belongs to the phenylpropanoid phytoalexin which is involved in plant response to various pathogen and herbivores attack. The aim of this study is to evaluate the anti-MRSA action of pterostilbene in combination with selected antibiotics; vancomycin, linezolid and oxacillin against ATCC 43300 and ATCC 33591. The minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and fractional inhibitory concentration (FIC) index values were determined. Microbroth dilution technique and microdilution checkerboard (MDC) assay were employed. The MIC and MBC of pterostilbene against ATCC 33591 was 31.25 and 62.50 µg mL-1, respectively. While for ATCC 43300, the MBC value was also twice (62.50 µg mL-1) its MIC value of 31.25 µg mL-1. This indicated that pterostilbene was bacteriostatic against both MRSA strains. Our MIC/MBC study also showed that linezolid exhibited bacteriostatic action but, oxacillin and vancomycin were bactericidal. MDC study showed that pterostilbene-oxacillin combination exhibited lowest FIC value (0.56) against both MRSA strains which indicated partial synergistic interaction. On the other hand, pterostilbene was additive (FIC 1.00) in combination with vancomycin whereas pterostilbene-linezolid combination displayed indifference effect with FIC of 1.25 against both MRSA strains. Pterostilbene in combination with oxacillin partially enhanced anti-MRSA activity with twofold reduction in MIC of oxacillin by acting at different site at the bacterial cell wall from that of oxacillin but more specific to the site of action of vancomycin.
KW - Bacteriostatic
KW - FIC
KW - MIC
KW - MRSA
KW - Pterostilbene
KW - Synergism
UR - http://www.scopus.com/inward/record.url?scp=84963567687&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84963567687&partnerID=8YFLogxK
U2 - 10.3844/ajidsp.2016.1.10
DO - 10.3844/ajidsp.2016.1.10
M3 - Article
VL - 12
SP - 1
EP - 10
JO - Revista Brasileira de Gestao e Desenvolvimento Regional
T2 - Revista Brasileira de Gestao e Desenvolvimento Regional
JF - Revista Brasileira de Gestao e Desenvolvimento Regional
SN - 1809-239X
IS - 1
ER -