Proteomic identification of host and parasite biomarkers in saliva from patients with uncomplicated Plasmodium falciparum malaria

Honglei Huang, Muhammad Mukram Mohamed Mackeen, Matthew Cook, Eniyou Oriero, Emily Locke, Marie L. Thézénas, Benedikt M. Kessler, Davis Nwakanma, Climent Casals-Pascual

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Background: Malaria cases attributed to Plasmodium falciparum account for approximately 600,000 deaths yearly, mainly in African children. The gold standard method to diagnose malaria requires the visualization of the parasite in blood. The role of non-invasive diagnostic methods to diagnose malaria remains unclear. Methods. A protocol was optimized to deplete highly abundant proteins from saliva to improve the dynamic range of the proteins identified and assess their suitability as candidate biomarkers of malaria infection. A starch-based amylase depletion strategy was used in combination with four different lectins to deplete glycoproteins (Concanavalin A and Aleuria aurantia for N-linked glycoproteins; jacalin and peanut agglutinin for O-linked glycoproteins). A proteomic analysis of depleted saliva samples was performed in 17 children with fever and a positive-malaria sliDe and compared with that of 17 malaria-negative children with fever. Results: The proteomic signature of malaria-positive patients revealed a strong up-regulation of erythrocyte-derived and inflammatory proteins. Three P. falciparum proteins, PFL0480w, PF08-0054 and PFI0875w, were identified in malaria patients and not in controls. Aleuria aurantia and jacalin showed the best results for parasite protein identification. Conclusions: This study shows that saliva is a suitable clinical specimen for biomarker discovery. Parasite proteins and several potential biomarkers were identified in patients with malaria but not in patients with other causes of fever. The diagnostic performance of these markers should be addressed prospectively.

Original languageEnglish
Article number178
JournalMalaria Journal
Volume11
DOIs
Publication statusPublished - 2012
Externally publishedYes

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Falciparum Malaria
Saliva
Proteomics
Malaria
Parasites
Biomarkers
Glycoproteins
Proteins
Fever
Plasmodium falciparum
Peanut Agglutinin
Amylases
Concanavalin A
Lectins
Starch
Up-Regulation
Erythrocytes

ASJC Scopus subject areas

  • Infectious Diseases
  • Parasitology

Cite this

Proteomic identification of host and parasite biomarkers in saliva from patients with uncomplicated Plasmodium falciparum malaria. / Huang, Honglei; Mohamed Mackeen, Muhammad Mukram; Cook, Matthew; Oriero, Eniyou; Locke, Emily; Thézénas, Marie L.; Kessler, Benedikt M.; Nwakanma, Davis; Casals-Pascual, Climent.

In: Malaria Journal, Vol. 11, 178, 2012.

Research output: Contribution to journalArticle

Huang, Honglei ; Mohamed Mackeen, Muhammad Mukram ; Cook, Matthew ; Oriero, Eniyou ; Locke, Emily ; Thézénas, Marie L. ; Kessler, Benedikt M. ; Nwakanma, Davis ; Casals-Pascual, Climent. / Proteomic identification of host and parasite biomarkers in saliva from patients with uncomplicated Plasmodium falciparum malaria. In: Malaria Journal. 2012 ; Vol. 11.
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