Proteomic analysis reveals that treatment with tocotrienols reverses the effect of H 2O 2 exposure on peroxiredoxin expression in human lymphocytes from young and old individuals

Hasnizawati Mohamed Dahlan, Saiful Anuar Karsani, Mariati Abdul Rahman, Noor Aini Abdul Hamid, A. Gapor Mat Top, Wan Zurinah Wan Ngah

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Vitamin E has been suggested to modulate age-associated changes by altering the redox balance resulting in altered gene and/or protein expression. Here we have utilized proteomics to determine whether such regulation in protein expression occurs in human lymphocytes from two different age groups stressed with H 2O 2 and then treated with vitamin E in the form of tocotrienol-rich fraction (TRF). In this study, lymphocytes obtained from young (30-49 years old) and old (>50 years old) volunteers were first challenged with 1 mM H 2O 2. They were then treated by exposure to 50, 100 and 200 μg/ml TRF. Two-dimensional gel electrophoresis followed by MALDI-TOF/TOF (matrix-assisted laser desorption/ionization time-of-flight/time-of-flight) tandem mass spectrometry was then performed on whole-cell protein extracts to identify proteins that have changed in expression. A total of 24 proteins were found to be affected by H 2O 2 and/or TRF treatment. These included proteins that were related to metabolism, antioxidants, structural proteins, protein degradation and signal transduction. Of particular interest was the regulation of a number of proteins involved in stress response-peroxiredoxin-2, peroxiredoxin-3 and peroxiredoxin-6-all of which were shown to be down-regulated with H 2O 2 exposure. The effect was reversed following TRF treatment. The expression of peroxiredoxin-2 and peroxiredoxin-6 was confirmed by quantitative reverse transcriptase polymerase chain reaction. These results suggested that TRF directly influenced the expression dynamics of the peroxiredoxin-2, thus improving the cells ability to resist damage caused by oxidative stress.

Original languageEnglish
Pages (from-to)741-751
Number of pages11
JournalJournal of Nutritional Biochemistry
Volume23
Issue number7
DOIs
Publication statusPublished - Jul 2012

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Tocotrienols
Peroxiredoxins
Lymphocytes
Proteomics
Peroxiredoxin VI
Proteins
Therapeutics
Vitamin E
Peroxiredoxin III
Signal transduction
Electrophoresis, Gel, Two-Dimensional
Oxidative stress
Tandem Mass Spectrometry
Cell Extracts
Reverse Transcriptase Polymerase Chain Reaction
Polymerase chain reaction
RNA-Directed DNA Polymerase
Proteolysis
Oxidation-Reduction
Electrophoresis

Keywords

  • Age-related changes
  • Lymphocyte
  • Oxidative stress
  • Tocotrienol-rich fraction
  • Two-dimensional electrophoresis

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Molecular Biology
  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics

Cite this

Proteomic analysis reveals that treatment with tocotrienols reverses the effect of H 2O 2 exposure on peroxiredoxin expression in human lymphocytes from young and old individuals. / Dahlan, Hasnizawati Mohamed; Karsani, Saiful Anuar; Abdul Rahman, Mariati; Hamid, Noor Aini Abdul; Top, A. Gapor Mat; Ngah, Wan Zurinah Wan.

In: Journal of Nutritional Biochemistry, Vol. 23, No. 7, 07.2012, p. 741-751.

Research output: Contribution to journalArticle

Dahlan, Hasnizawati Mohamed ; Karsani, Saiful Anuar ; Abdul Rahman, Mariati ; Hamid, Noor Aini Abdul ; Top, A. Gapor Mat ; Ngah, Wan Zurinah Wan. / Proteomic analysis reveals that treatment with tocotrienols reverses the effect of H 2O 2 exposure on peroxiredoxin expression in human lymphocytes from young and old individuals. In: Journal of Nutritional Biochemistry. 2012 ; Vol. 23, No. 7. pp. 741-751.
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