Proteomic analysis during capsicum ripening reveals differential expression of ACC oxidase isoform 4 and other candidates

Wan Mohd Aizat Wan Kamaruddin, Jason A. Able, James C R Stangoulis, Amanda J. Able

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Capsicum (Capsicum annuum L.) is categorised as a non-climacteric fruit that exhibits limited ethylene production during ripening and the molecular mechanisms associated with this process are poorly understood. A proteomic approach was used to identify the differentially expressed proteins during various ripening stages (Green (G), Breaker Red 1 (BR1) and Light Red (LR)) and the genes associated with their synthesis. From 2D gel electrophoresis (2DGE), seven protein spots were identified as selectively present either in G or BR1 and are involved in carbon metabolism, colour and fruit development, protein synthesis and chaperones or biosynthesis of amino acids and polyamines. One candidate of interest, 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase (ACO) is known to be involved in ethylene biosynthesis and was only present in BR1 and is related to the tomato ACO isoform 4 (LeACO4) and hence named CaACO4. CaACO4 RNA expression as well as total ACO protein expression in multiple stages of ripening (G, Breaker (B), BR1, Breaker Red 2 (BR2), LR and Deep Red (DR)) corresponded to the 2DGE protein spot abundance in breaker stages. Our findings highlight the involvement of the ethylene pathway in non-climacteric fruit ripening.

Original languageEnglish
Pages (from-to)1115-1128
Number of pages14
JournalFunctional Plant Biology
Volume40
Issue number11
DOIs
Publication statusPublished - 2013
Externally publishedYes

Fingerprint

Capsicum
1-aminocyclopropane-1-carboxylic acid
aminocyclopropanecarboxylate oxidase
proteomics
ripening
ethylene production
gel electrophoresis
protein synthesis
fruits
proteins
Capsicum annuum
polyamines
fruiting
ethylene
tomatoes
biosynthesis
RNA
amino acids
metabolism
synthesis

Keywords

  • 2D gel electrophoresis
  • Capsicum annuum
  • fruit ripening
  • non-climacteric ripening
  • pepper
  • proteomics

ASJC Scopus subject areas

  • Plant Science
  • Agronomy and Crop Science

Cite this

Proteomic analysis during capsicum ripening reveals differential expression of ACC oxidase isoform 4 and other candidates. / Wan Kamaruddin, Wan Mohd Aizat; Able, Jason A.; Stangoulis, James C R; Able, Amanda J.

In: Functional Plant Biology, Vol. 40, No. 11, 2013, p. 1115-1128.

Research output: Contribution to journalArticle

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