Proteome profiling in the hippocampus, medial prefrontal cortex, and striatum of aging rat

Hamizah Shahirah Hamezah, Lina Wati Durani, Daijiro Yanagisawa, Nor Faeizah Ibrahim, Wan Mohd Aizat Wan Kamaruddin, Jean Pierre Bellier, Suzana Makpol, Wan Zurinah Wan Ngah, Mohd Hanafi Ahmad Damanhuri, Ikuo Tooyama

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Decrease in multiple functions occurs in the brain with aging, all of which can contribute to age-related cognitive and locomotor impairments. Brain atrophy specifically in hippocampus, medial prefrontal cortex (mPFC), and striatum, can contribute to this age-associated decline in function. Our recent metabolomics analysis showed age-related changes in these brain regions. To further understand the aging processes, analysis using a proteomics approach was carried out. This study was conducted to identify proteome profiles in the hippocampus, mPFC, and striatum of 14-, 18-, 23-, and 27-month-old rats. Proteomics analysis using ultrahigh performance liquid chromatography coupled with Q Exactive HF Orbitrap mass spectrometry identified 1074 proteins in the hippocampus, 871 proteins in the mPFC, and 241 proteins in the striatum. Of these proteins, 97 in the hippocampus, 25 in mPFC, and 5 in striatum were differentially expressed with age. The altered proteins were classified into three ontologies (cellular component, molecular function, and biological process) containing 44, 38, and 35 functional groups in the hippocampus, mPFC, and striatum, respectively. Most of these altered proteins participate in oxidative phosphorylation (e.g. cytochrome c oxidase and ATP synthase), glutathione metabolism (e.g. peroxiredoxins), or calcium signaling pathway (e.g. protein S100B and calmodulin). The most prominent changes were observed in the oldest animals. These results suggest that alterations in oxidative phosphorylation, glutathione metabolism, and calcium signaling pathway are involved in cognitive and locomotor impairments in aging.

Original languageEnglish
Pages (from-to)53-64
Number of pages12
JournalExperimental Gerontology
Volume111
DOIs
Publication statusPublished - 1 Oct 2018

Fingerprint

Proteome
Prefrontal Cortex
Rats
Hippocampus
Aging of materials
Proteins
Brain
Calcium Signaling
Oxidative Phosphorylation
Metabolism
Proteomics
Glutathione Synthase
Calcium
Peroxiredoxins
Biological Phenomena
Metabolomics
Liquid chromatography
Electron Transport Complex IV
Calmodulin
Liquid Chromatography

Keywords

  • Aging
  • Brain
  • Proteomics

ASJC Scopus subject areas

  • Biochemistry
  • Ageing
  • Molecular Biology
  • Genetics
  • Endocrinology
  • Cell Biology

Cite this

Proteome profiling in the hippocampus, medial prefrontal cortex, and striatum of aging rat. / Hamezah, Hamizah Shahirah; Durani, Lina Wati; Yanagisawa, Daijiro; Ibrahim, Nor Faeizah; Wan Kamaruddin, Wan Mohd Aizat; Bellier, Jean Pierre; Makpol, Suzana; Ngah, Wan Zurinah Wan; Ahmad Damanhuri, Mohd Hanafi; Tooyama, Ikuo.

In: Experimental Gerontology, Vol. 111, 01.10.2018, p. 53-64.

Research output: Contribution to journalArticle

Hamezah, Hamizah Shahirah ; Durani, Lina Wati ; Yanagisawa, Daijiro ; Ibrahim, Nor Faeizah ; Wan Kamaruddin, Wan Mohd Aizat ; Bellier, Jean Pierre ; Makpol, Suzana ; Ngah, Wan Zurinah Wan ; Ahmad Damanhuri, Mohd Hanafi ; Tooyama, Ikuo. / Proteome profiling in the hippocampus, medial prefrontal cortex, and striatum of aging rat. In: Experimental Gerontology. 2018 ; Vol. 111. pp. 53-64.
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AU - Bellier, Jean Pierre

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