Abstract
Decrease in multiple functions occurs in the brain with aging, all of which can contribute to age-related cognitive and locomotor impairments. Brain atrophy specifically in hippocampus, medial prefrontal cortex (mPFC), and striatum, can contribute to this age-associated decline in function. Our recent metabolomics analysis showed age-related changes in these brain regions. To further understand the aging processes, analysis using a proteomics approach was carried out. This study was conducted to identify proteome profiles in the hippocampus, mPFC, and striatum of 14-, 18-, 23-, and 27-month-old rats. Proteomics analysis using ultrahigh performance liquid chromatography coupled with Q Exactive HF Orbitrap mass spectrometry identified 1074 proteins in the hippocampus, 871 proteins in the mPFC, and 241 proteins in the striatum. Of these proteins, 97 in the hippocampus, 25 in mPFC, and 5 in striatum were differentially expressed with age. The altered proteins were classified into three ontologies (cellular component, molecular function, and biological process) containing 44, 38, and 35 functional groups in the hippocampus, mPFC, and striatum, respectively. Most of these altered proteins participate in oxidative phosphorylation (e.g. cytochrome c oxidase and ATP synthase), glutathione metabolism (e.g. peroxiredoxins), or calcium signaling pathway (e.g. protein S100B and calmodulin). The most prominent changes were observed in the oldest animals. These results suggest that alterations in oxidative phosphorylation, glutathione metabolism, and calcium signaling pathway are involved in cognitive and locomotor impairments in aging.
| Original language | English |
|---|---|
| Pages (from-to) | 53-64 |
| Number of pages | 12 |
| Journal | Experimental Gerontology |
| Volume | 111 |
| DOIs | |
| Publication status | Published - 1 Oct 2018 |
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Keywords
- Aging
- Brain
- Proteomics
ASJC Scopus subject areas
- Biochemistry
- Ageing
- Molecular Biology
- Genetics
- Endocrinology
- Cell Biology
Cite this
Proteome profiling in the hippocampus, medial prefrontal cortex, and striatum of aging rat. / Hamezah, Hamizah Shahirah; Durani, Lina Wati; Yanagisawa, Daijiro; Ibrahim, Nor Faeizah; Wan Kamaruddin, Wan Mohd Aizat; Bellier, Jean Pierre; Makpol, Suzana; Ngah, Wan Zurinah Wan; Ahmad Damanhuri, Mohd Hanafi; Tooyama, Ikuo.
In: Experimental Gerontology, Vol. 111, 01.10.2018, p. 53-64.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Proteome profiling in the hippocampus, medial prefrontal cortex, and striatum of aging rat
AU - Hamezah, Hamizah Shahirah
AU - Durani, Lina Wati
AU - Yanagisawa, Daijiro
AU - Ibrahim, Nor Faeizah
AU - Wan Kamaruddin, Wan Mohd Aizat
AU - Bellier, Jean Pierre
AU - Makpol, Suzana
AU - Ngah, Wan Zurinah Wan
AU - Ahmad Damanhuri, Mohd Hanafi
AU - Tooyama, Ikuo
PY - 2018/10/1
Y1 - 2018/10/1
N2 - Decrease in multiple functions occurs in the brain with aging, all of which can contribute to age-related cognitive and locomotor impairments. Brain atrophy specifically in hippocampus, medial prefrontal cortex (mPFC), and striatum, can contribute to this age-associated decline in function. Our recent metabolomics analysis showed age-related changes in these brain regions. To further understand the aging processes, analysis using a proteomics approach was carried out. This study was conducted to identify proteome profiles in the hippocampus, mPFC, and striatum of 14-, 18-, 23-, and 27-month-old rats. Proteomics analysis using ultrahigh performance liquid chromatography coupled with Q Exactive HF Orbitrap mass spectrometry identified 1074 proteins in the hippocampus, 871 proteins in the mPFC, and 241 proteins in the striatum. Of these proteins, 97 in the hippocampus, 25 in mPFC, and 5 in striatum were differentially expressed with age. The altered proteins were classified into three ontologies (cellular component, molecular function, and biological process) containing 44, 38, and 35 functional groups in the hippocampus, mPFC, and striatum, respectively. Most of these altered proteins participate in oxidative phosphorylation (e.g. cytochrome c oxidase and ATP synthase), glutathione metabolism (e.g. peroxiredoxins), or calcium signaling pathway (e.g. protein S100B and calmodulin). The most prominent changes were observed in the oldest animals. These results suggest that alterations in oxidative phosphorylation, glutathione metabolism, and calcium signaling pathway are involved in cognitive and locomotor impairments in aging.
AB - Decrease in multiple functions occurs in the brain with aging, all of which can contribute to age-related cognitive and locomotor impairments. Brain atrophy specifically in hippocampus, medial prefrontal cortex (mPFC), and striatum, can contribute to this age-associated decline in function. Our recent metabolomics analysis showed age-related changes in these brain regions. To further understand the aging processes, analysis using a proteomics approach was carried out. This study was conducted to identify proteome profiles in the hippocampus, mPFC, and striatum of 14-, 18-, 23-, and 27-month-old rats. Proteomics analysis using ultrahigh performance liquid chromatography coupled with Q Exactive HF Orbitrap mass spectrometry identified 1074 proteins in the hippocampus, 871 proteins in the mPFC, and 241 proteins in the striatum. Of these proteins, 97 in the hippocampus, 25 in mPFC, and 5 in striatum were differentially expressed with age. The altered proteins were classified into three ontologies (cellular component, molecular function, and biological process) containing 44, 38, and 35 functional groups in the hippocampus, mPFC, and striatum, respectively. Most of these altered proteins participate in oxidative phosphorylation (e.g. cytochrome c oxidase and ATP synthase), glutathione metabolism (e.g. peroxiredoxins), or calcium signaling pathway (e.g. protein S100B and calmodulin). The most prominent changes were observed in the oldest animals. These results suggest that alterations in oxidative phosphorylation, glutathione metabolism, and calcium signaling pathway are involved in cognitive and locomotor impairments in aging.
KW - Aging
KW - Brain
KW - Proteomics
UR - http://www.scopus.com/inward/record.url?scp=85049925145&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85049925145&partnerID=8YFLogxK
U2 - 10.1016/j.exger.2018.07.002
DO - 10.1016/j.exger.2018.07.002
M3 - Article
C2 - 29981398
AN - SCOPUS:85049925145
VL - 111
SP - 53
EP - 64
JO - Experimental Gerontology
JF - Experimental Gerontology
SN - 0531-5565
ER -