Protective role of Hibiscus sabdariffa calyx extract against streptozotocin induced sperm damage in diabetic rats

Muhd Hanis Md Idris, Siti Balkis Budin, Mohamad Osman, Jamaludin Mohamed

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Diabetes mellitus contributes to male sexual dysfunction and infertility by modulating oxidative damage. To date, a number of studies have demonstrated antioxidant properties of Hibiscus sabdariffa Linn. This study was designed to investigate the effects of H. sabdariffa UKMR-2 variety on sperm functioning of streptozotocin-induced diabetic rats. Male Sprague- Dawley rats were allotted into four groups, namely control group (C), H. sabdariffa extract (HSE) group, diabetes group (D) and diabetes plus HSE group (D+HSE). HSE (100 mg/ kg/body weight) was administered orally for 28 consecutive days. After 28-days of supplementation, the rats were sacrificed to obtain epididymal sperm. Administration of HSE significantly lowered the level of fasting blood glucose and increased plasma insulin level in D+HSE group as compared to D group (p<0.05). Sperm quality in the D+HSE group was improved with significantly higher sperm concentrations (p<0.05) and sperm motility (p<0.001) as well as lower percentage of sperm abnormality (p<0.05) as compared to the diabetic group. Plasma follicle-stimulating hormone (FSH) level was significantly elevated (p<0.05) in D+HSE group than in D group while no significant alteration in plasma testosterone and luteinizing hormone (LH) level were seen between groups. In conclusion, this study suggested that H. sabdariffa UKMR-2 variety has a potential protective role against diabetes-induced sperm damage.

Original languageEnglish
Pages (from-to)659-669
Number of pages11
JournalEXCLI Journal
Volume11
Publication statusPublished - 2012

Fingerprint

Hibiscus
Hibiscus sabdariffa
streptozotocin
Streptozocin
calyx
Spermatozoa
spermatozoa
rats
extracts
diabetes
Sperm Motility
follicle-stimulating hormone
sperm concentration
Follicle Stimulating Hormone
luteinizing hormone
diabetes mellitus
Luteinizing Hormone
sperm motility
Infertility
blood glucose

Keywords

  • Diabetes
  • Hibiscus sabdariffa UKMR-2
  • Oxidative stress
  • Sperm damage

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology
  • Drug Discovery
  • Animal Science and Zoology

Cite this

Protective role of Hibiscus sabdariffa calyx extract against streptozotocin induced sperm damage in diabetic rats. / Md Idris, Muhd Hanis; Budin, Siti Balkis; Osman, Mohamad; Mohamed, Jamaludin.

In: EXCLI Journal, Vol. 11, 2012, p. 659-669.

Research output: Contribution to journalArticle

@article{78220bcf67224003ab292145cda1578d,
title = "Protective role of Hibiscus sabdariffa calyx extract against streptozotocin induced sperm damage in diabetic rats",
abstract = "Diabetes mellitus contributes to male sexual dysfunction and infertility by modulating oxidative damage. To date, a number of studies have demonstrated antioxidant properties of Hibiscus sabdariffa Linn. This study was designed to investigate the effects of H. sabdariffa UKMR-2 variety on sperm functioning of streptozotocin-induced diabetic rats. Male Sprague- Dawley rats were allotted into four groups, namely control group (C), H. sabdariffa extract (HSE) group, diabetes group (D) and diabetes plus HSE group (D+HSE). HSE (100 mg/ kg/body weight) was administered orally for 28 consecutive days. After 28-days of supplementation, the rats were sacrificed to obtain epididymal sperm. Administration of HSE significantly lowered the level of fasting blood glucose and increased plasma insulin level in D+HSE group as compared to D group (p<0.05). Sperm quality in the D+HSE group was improved with significantly higher sperm concentrations (p<0.05) and sperm motility (p<0.001) as well as lower percentage of sperm abnormality (p<0.05) as compared to the diabetic group. Plasma follicle-stimulating hormone (FSH) level was significantly elevated (p<0.05) in D+HSE group than in D group while no significant alteration in plasma testosterone and luteinizing hormone (LH) level were seen between groups. In conclusion, this study suggested that H. sabdariffa UKMR-2 variety has a potential protective role against diabetes-induced sperm damage.",
keywords = "Diabetes, Hibiscus sabdariffa UKMR-2, Oxidative stress, Sperm damage",
author = "{Md Idris}, {Muhd Hanis} and Budin, {Siti Balkis} and Mohamad Osman and Jamaludin Mohamed",
year = "2012",
language = "English",
volume = "11",
pages = "659--669",
journal = "EXCLI Journal",
issn = "1611-2156",
publisher = "University of Mainz",

}

TY - JOUR

T1 - Protective role of Hibiscus sabdariffa calyx extract against streptozotocin induced sperm damage in diabetic rats

AU - Md Idris, Muhd Hanis

AU - Budin, Siti Balkis

AU - Osman, Mohamad

AU - Mohamed, Jamaludin

PY - 2012

Y1 - 2012

N2 - Diabetes mellitus contributes to male sexual dysfunction and infertility by modulating oxidative damage. To date, a number of studies have demonstrated antioxidant properties of Hibiscus sabdariffa Linn. This study was designed to investigate the effects of H. sabdariffa UKMR-2 variety on sperm functioning of streptozotocin-induced diabetic rats. Male Sprague- Dawley rats were allotted into four groups, namely control group (C), H. sabdariffa extract (HSE) group, diabetes group (D) and diabetes plus HSE group (D+HSE). HSE (100 mg/ kg/body weight) was administered orally for 28 consecutive days. After 28-days of supplementation, the rats were sacrificed to obtain epididymal sperm. Administration of HSE significantly lowered the level of fasting blood glucose and increased plasma insulin level in D+HSE group as compared to D group (p<0.05). Sperm quality in the D+HSE group was improved with significantly higher sperm concentrations (p<0.05) and sperm motility (p<0.001) as well as lower percentage of sperm abnormality (p<0.05) as compared to the diabetic group. Plasma follicle-stimulating hormone (FSH) level was significantly elevated (p<0.05) in D+HSE group than in D group while no significant alteration in plasma testosterone and luteinizing hormone (LH) level were seen between groups. In conclusion, this study suggested that H. sabdariffa UKMR-2 variety has a potential protective role against diabetes-induced sperm damage.

AB - Diabetes mellitus contributes to male sexual dysfunction and infertility by modulating oxidative damage. To date, a number of studies have demonstrated antioxidant properties of Hibiscus sabdariffa Linn. This study was designed to investigate the effects of H. sabdariffa UKMR-2 variety on sperm functioning of streptozotocin-induced diabetic rats. Male Sprague- Dawley rats were allotted into four groups, namely control group (C), H. sabdariffa extract (HSE) group, diabetes group (D) and diabetes plus HSE group (D+HSE). HSE (100 mg/ kg/body weight) was administered orally for 28 consecutive days. After 28-days of supplementation, the rats were sacrificed to obtain epididymal sperm. Administration of HSE significantly lowered the level of fasting blood glucose and increased plasma insulin level in D+HSE group as compared to D group (p<0.05). Sperm quality in the D+HSE group was improved with significantly higher sperm concentrations (p<0.05) and sperm motility (p<0.001) as well as lower percentage of sperm abnormality (p<0.05) as compared to the diabetic group. Plasma follicle-stimulating hormone (FSH) level was significantly elevated (p<0.05) in D+HSE group than in D group while no significant alteration in plasma testosterone and luteinizing hormone (LH) level were seen between groups. In conclusion, this study suggested that H. sabdariffa UKMR-2 variety has a potential protective role against diabetes-induced sperm damage.

KW - Diabetes

KW - Hibiscus sabdariffa UKMR-2

KW - Oxidative stress

KW - Sperm damage

UR - http://www.scopus.com/inward/record.url?scp=84867609118&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84867609118&partnerID=8YFLogxK

M3 - Article

VL - 11

SP - 659

EP - 669

JO - EXCLI Journal

JF - EXCLI Journal

SN - 1611-2156

ER -