Protective effects of Zingiber zerumbet ethyl acetate extract on hydrogen peroxide-induced damage of red blood cells

Mohd Fuad Rahmat Sam, Asmah Hamid, Ahmad Rohi Ghazali, Santhana Raj Louis, Siti Balkis Budin

Research output: Contribution to journalArticle

Abstract

Zingiber zerumbet is widely used as therapeutic agent in traditional medicine and is reported to exert antioxidant activities. This study evaluated in vitro antioxidant potential of Z. zerumbet rhizome ethyl acetate extract on hydrogen peroxide (H 2 O 2 )-induced red blood cells (RBCs) damage; by measuring percentage of haemolysis, oxidative damage and morphological changes in treated RBCs. On a preliminary dose-response analysis, it was observed that incubation of RBCs with extract at doses of 50, 25, 12.5 and 6.25 µg/mL all had no significant effect on RBCs similar to NaCl control and BHT (positive control), therefore, these concentrations were used in the subsequent study. The RBCs were pre-incubated with extract in the chosen concentrations, prior to treatment with H 2 O 2 . The results showed that only 6.25 µg/mL+H 2 O 2 group showed significantly (p<0.05) lower percentage of haemolysis and oxidative damage; indicated by low level of malondialdehyde (MDA) and protein carbonyls (PC) compared to the H 2 O 2 alone group. Electron microscopic examination further showed that pre-treatment with 6.25 µg/mL extract reduced the H 2 O 2 –induced morphological changes of RBCs. Phytochemical analysis of extract using Gas Chromatography Mass Spectrometry (GCMS) identified Zerumbone as the highest constituent compound (51.57%) in this extract. In conclusion, this study indicated that 6.25 µg/mL ethyl acetate extract Z. zerumbet rhizome could efficiently protect RBCs against oxidative damage induced by H 2 O 2 and this effects could possibly due the high constituent of Zerumbone.

Original languageEnglish
Pages (from-to)781-790
Number of pages10
JournalSains Malaysiana
Volume48
Issue number4
DOIs
Publication statusPublished - 1 Jan 2019

Fingerprint

Hydrogen Peroxide
Blood
Cells
Antioxidants
Butylated Hydroxytoluene
Phytochemicals
ethyl acetate
Malondialdehyde
Gas chromatography
Medicine
Mass spectrometry
Microscopic examination
Electrons
Proteins

Keywords

  • Haemolysis
  • Lipid peroxidation
  • Red blood cells
  • Zingiber zerumbet

ASJC Scopus subject areas

  • General

Cite this

Protective effects of Zingiber zerumbet ethyl acetate extract on hydrogen peroxide-induced damage of red blood cells. / Sam, Mohd Fuad Rahmat; Hamid, Asmah; Ghazali, Ahmad Rohi; Louis, Santhana Raj; Budin, Siti Balkis.

In: Sains Malaysiana, Vol. 48, No. 4, 01.01.2019, p. 781-790.

Research output: Contribution to journalArticle

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