Protective effects of Tualang honey on bone structure in experimental postmenopausal rats

Siti Sarah Mohamad Zaid, Siti Amrah Sulaiman, Nor Hayati Othman, Ima Nirwana Soelaiman, Ahmad Nazrun Shuid, Norazlina Mohamed, Norliza Muhammad

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Objective: The objective of this study was to evaluate the effects of Tualang honey on trabecular structure and compare these effects with those of calcium supplementation in ovariectomized rats. Methods: Forty female, Sprague-Dawley rats were randomly divided into five groups (n =8): four controls and one test arm. The control arm comprised a baseline control, sham-operated control, ovariectomized control, and ovariectomized calcium-treated rats (receiving 1% calcium in drinking water ad libitum). The test arm was composed of ovariectomized, Tualang honey-treated rats (received 0.2 g/kg body weight of Tualang honey). Both the sham-operated control and ovariectomized control groups received vehicle treatment (deionized water), and the baseline control group was sacrificed without treatment. Results: All rats were orally gavaged daily for six weeks after day one post-surgery. The bone structural analysis of rats in the test arm group showed a significant increase in the bone volume per tissue volume (BV/TV), trabecular thickness (Tb.Th) and trabecular number (Tb.N) and a significant decrease in inter-trabecular space (Tb.Sp) compared with the ovariectomized control group. The trabecular thickness (Tb.Th) in the test arm group was significantly higher compared with the ovariectomized-calcium treated group, and the inter-trabecular space (Tb.Sp) in the test arm group was significantly narrower compared with the ovariectomized-calcium treated group. Conclusion: In conclusion, ovariectomized rats that received Tualang honey showed more improvements in trabecular bone structure than the rats that received calcium.

Original languageEnglish
Pages (from-to)779-784
Number of pages6
JournalClinics
Volume67
Issue number7
DOIs
Publication statusPublished - Jul 2012

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Honey
Bone and Bones
Calcium
Control Groups
Water Purification
Drinking Water
Sprague Dawley Rats
Body Weight

Keywords

  • Osteoporosis
  • Ovariectomy
  • Structural
  • Trabecular
  • Tualang honey

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Protective effects of Tualang honey on bone structure in experimental postmenopausal rats. / Mohamad Zaid, Siti Sarah; Sulaiman, Siti Amrah; Othman, Nor Hayati; Soelaiman, Ima Nirwana; Shuid, Ahmad Nazrun; Mohamed, Norazlina; Muhammad, Norliza.

In: Clinics, Vol. 67, No. 7, 07.2012, p. 779-784.

Research output: Contribution to journalArticle

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abstract = "Objective: The objective of this study was to evaluate the effects of Tualang honey on trabecular structure and compare these effects with those of calcium supplementation in ovariectomized rats. Methods: Forty female, Sprague-Dawley rats were randomly divided into five groups (n =8): four controls and one test arm. The control arm comprised a baseline control, sham-operated control, ovariectomized control, and ovariectomized calcium-treated rats (receiving 1{\%} calcium in drinking water ad libitum). The test arm was composed of ovariectomized, Tualang honey-treated rats (received 0.2 g/kg body weight of Tualang honey). Both the sham-operated control and ovariectomized control groups received vehicle treatment (deionized water), and the baseline control group was sacrificed without treatment. Results: All rats were orally gavaged daily for six weeks after day one post-surgery. The bone structural analysis of rats in the test arm group showed a significant increase in the bone volume per tissue volume (BV/TV), trabecular thickness (Tb.Th) and trabecular number (Tb.N) and a significant decrease in inter-trabecular space (Tb.Sp) compared with the ovariectomized control group. The trabecular thickness (Tb.Th) in the test arm group was significantly higher compared with the ovariectomized-calcium treated group, and the inter-trabecular space (Tb.Sp) in the test arm group was significantly narrower compared with the ovariectomized-calcium treated group. Conclusion: In conclusion, ovariectomized rats that received Tualang honey showed more improvements in trabecular bone structure than the rats that received calcium.",
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AU - Shuid, Ahmad Nazrun

AU - Mohamed, Norazlina

AU - Muhammad, Norliza

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