Protective effects of Labisia pumila var. alata on biochemical and histopathological alterations of cardiac muscle cells in isoproterenol-induced myocardial infarction rats

Roza Dianita, Ibrahim Jantan, Athirah Z. Amran, Juriyati Jalil

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The study was designed to evaluate the cardioprotective effects of the standardized aqueous and 80% ethanol extracts of Labisia pumila var. alata (LPva) in isoproterenol (ISO)-induced myocardial infarction (MI) in rats. The extracts were administered to Wistar rats orally for 28 days with three doses (100, 200 and 400 mg/kg of body weight) prior to ISO (85 mg/kg)-induced MI in two doses on day 29 and 30. The sera and hearts were collected for biochemical and histopathological analysis after the rats were sacrificed 48 h after the first induction. The main components of the extracts, gallic acid, alkylresorcinols and flavonoids were identified and quantitatively analyzed in the extracts by using a validated reversed phase HPLC method. The extracts showed significant protective effects as pretreated rats showed a significant dose-dependent decrease (p < 0.05) in cardiac enzyme activities, i.e., cardiac troponin I (cTnI), creatine kinase MB isoenzyme (CK-MB), lactate dehydrogenase (LDH), alanine transaminase (ALT) and aspartate transaminase (AST), when compared with ISO-control rats. There were significant rises (p < 0.05) in the activity of oxidase enzymes, i.e., glutathione peroxide (GPx), catalase (CAT) and superoxide dismutase (SOD) of the pretreated rats, when compared with ISO-control group. Histopathological examination showed an improvement in membrane cell integrity in pre-treated rats compared to untreated rats. The major components of LPva extracts can be used as their biomarkers and contributed to the cardioprotective effects against ISO-induced MI rats.

Original languageEnglish
Pages (from-to)4746-4763
Number of pages18
JournalMolecules
Volume20
Issue number3
DOIs
Publication statusPublished - 1 Mar 2015

Fingerprint

myocardial infarction
muscle cells
Isoproterenol
Cardiac Myocytes
rats
Muscle
Rats
Myocardial Infarction
Cells
dosage
Rat control
creatine
MB Form Creatine Kinase
Gallic Acid
Troponin I
aspartates
catalase
body weight
Peroxides
enzyme activity

Keywords

  • Alkylresorsinols
  • Cardioprotection
  • Isoproterenol
  • Labisia pumila var. alata
  • Myocardial infarction
  • Standardized extracts

ASJC Scopus subject areas

  • Organic Chemistry

Cite this

Protective effects of Labisia pumila var. alata on biochemical and histopathological alterations of cardiac muscle cells in isoproterenol-induced myocardial infarction rats. / Dianita, Roza; Jantan, Ibrahim; Amran, Athirah Z.; Jalil, Juriyati.

In: Molecules, Vol. 20, No. 3, 01.03.2015, p. 4746-4763.

Research output: Contribution to journalArticle

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