Protective effects of gelam honey against oxidative damage in young and aged rats

Zulaikha Sahhugi, Siti Maisarah Hasenan, Zakiah Jubri

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Aging is characterized by progressive decline in physiological and body function due to increase in oxidative damage. Gelam honey has been accounted to have high phenolic and nonphenolic content to attenuate oxidative damage. This study was to determine the effect of local gelam honey on oxidative damage of aged rats. Twenty-four male Spraque-Dawley rats were divided into young (2 months) and aged (19 months) groups. Each group was further divided into control (fed with plain water) and supplemented with 2.5 mg/kg body weight of gelam honey for 8 months. DNA damage level was determined by comet assay and plasma malondialdehyde (MDA) by high performance liquid chromatography (HPLC). The activity of blood and cardiac antioxidant enzymes was determined by spectrophotometer. The DNA damage and MDA level were reduced in both gelam honey supplemented groups. Gelam honey increases erythrocytes CAT and cardiac SOD activities in young and cardiac CAT activity in young and aged groups. The DNA damage was increased in the aged group compared to young group, but reduced at the end of the study. The decline of oxidative damage in rats supplemented with gelam honey might be through the modulation of antioxidant enzyme activities.

Original languageEnglish
Article number673628
JournalOxidative Medicine and Cellular Longevity
Volume2014
DOIs
Publication statusPublished - 2014

Fingerprint

Honey
Rats
Malondialdehyde
DNA
Antioxidants
DNA Damage
Spectrophotometers
High performance liquid chromatography
Enzyme activity
Assays
Blood
Aging of materials
Modulation
Plasmas
Comet Assay
Water
Enzymes
Erythrocytes
High Pressure Liquid Chromatography
Body Weight

ASJC Scopus subject areas

  • Cell Biology
  • Ageing
  • Biochemistry

Cite this

Protective effects of gelam honey against oxidative damage in young and aged rats. / Sahhugi, Zulaikha; Hasenan, Siti Maisarah; Jubri, Zakiah.

In: Oxidative Medicine and Cellular Longevity, Vol. 2014, 673628, 2014.

Research output: Contribution to journalArticle

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