Protective effect of chlorella vulgaris on dna damage, oxidative stress, and lung morphological changes in cigarette smoke-exposed rats

K. S. Zamri, M. K.N. Norripin, F. I. Darus, D. G. Ekambaram, N. D. Abdul Raof, N. H. Roslan, Fairus Ahmad, F. Mohammed, Suzana Makpol, Yasmin Anum Mohd Yusof

Research output: Contribution to journalArticle

Abstract

Objective: The aim of this study was to determine the protective effect of Chlorella vulgaris (ChV), antioxidant-rich unicellular green alga, and in cigarette smoke-exposed rats. Methods: Male Sprague Dawley rats were divided into 4 groups: Control Group (C), ChV group (300 mg/kg body weight), cigarette smoke-exposed (S) group, and S group treated with ChV (S+ChV). Blood samples were drawn from the orbital sinus on days 0, 15, and 30 for the determination of DNA damage by Comet assay and plasma malondialdehyde (MDA) using high-performance liquid chromatography. Rats were killed on day 30, and lung tissue samples were taken for the evaluation of airspace enlargement and number of inflammatory cells. Results: Increased DNA damage (1004.8 au + 329.2, day 15; 1102.7 + 197.8, day 30) and high MDA levels (10.66 + 0.27, day 15; 10.29 + 0.9 day 30) were found in cigarette smoke-exposed rats on days 15 and 30 but were reduced significantly (p<0.05) when treated with ChV (DNA: 482.6 + 223.3, day15; 423.5 + 74.6, day 30 and MDA: 6.1 + 0.6, day15; 6.6 + 2.5, day 30) for both days. Hematoxylin and eosin staining showed that cigarette smoke-exposed rats had high frequency of airspace enlargement and number of inflammatory cells which were reduced when treated with ChV. Conclusion: ChV has a protective role in cigarette smoke-exposed rats by reducing oxidative DNA damage, MDA levels, lung cells inflammation, and airspace enlargement.

Original languageEnglish
Pages (from-to)145-149
Number of pages5
JournalAsian Journal of Pharmaceutical and Clinical Research
Volume11
Issue number10
DOIs
Publication statusPublished - 1 Oct 2018

Fingerprint

Chlorella vulgaris
Smoke
Tobacco Products
Oxidative Stress
Lung
Malondialdehyde
DNA Damage
Cell Count
Chlorophyta
Comet Assay
Hematoxylin
Eosine Yellowish-(YS)
Sprague Dawley Rats
Pneumonia
Antioxidants
High Pressure Liquid Chromatography
Body Weight
Staining and Labeling
Control Groups
DNA

Keywords

  • Airspace enlargement
  • Chlorella vulgaris
  • DNA damage
  • Inflammatory cells
  • Malondialdehyde

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science
  • Pharmacology (medical)

Cite this

Protective effect of chlorella vulgaris on dna damage, oxidative stress, and lung morphological changes in cigarette smoke-exposed rats. / Zamri, K. S.; Norripin, M. K.N.; Darus, F. I.; Ekambaram, D. G.; Abdul Raof, N. D.; Roslan, N. H.; Ahmad, Fairus; Mohammed, F.; Makpol, Suzana; Mohd Yusof, Yasmin Anum.

In: Asian Journal of Pharmaceutical and Clinical Research, Vol. 11, No. 10, 01.10.2018, p. 145-149.

Research output: Contribution to journalArticle

Zamri, K. S. ; Norripin, M. K.N. ; Darus, F. I. ; Ekambaram, D. G. ; Abdul Raof, N. D. ; Roslan, N. H. ; Ahmad, Fairus ; Mohammed, F. ; Makpol, Suzana ; Mohd Yusof, Yasmin Anum. / Protective effect of chlorella vulgaris on dna damage, oxidative stress, and lung morphological changes in cigarette smoke-exposed rats. In: Asian Journal of Pharmaceutical and Clinical Research. 2018 ; Vol. 11, No. 10. pp. 145-149.
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AU - Norripin, M. K.N.

AU - Darus, F. I.

AU - Ekambaram, D. G.

AU - Abdul Raof, N. D.

AU - Roslan, N. H.

AU - Ahmad, Fairus

AU - Mohammed, F.

AU - Makpol, Suzana

AU - Mohd Yusof, Yasmin Anum

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KW - Chlorella vulgaris

KW - DNA damage

KW - Inflammatory cells

KW - Malondialdehyde

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